Growth of oil accumulating microalga Neochloris oleoabundans under alkaline–saline conditions

Santos, Alisson Moura; Janssen, Marcel; Lamers, Packo P.; Evers, Wendy A. C.; Wijffels, René H.

doi:10.1016/j.biortech.2011.10.084

Cited by 103 publications

(35 citation statements)

References 27 publications

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“…The relative content of polyunsaturated FAs (PUFA), especially linolenic acid (18:3), on the other hand decreased substantially. These results was consistent with the previous report in freshwater Chlamydomonas mexicana and S. obtusus XJ-15 under high salinity (Salama et al, 2013;Xia et al, 2013), and Neochloris oleoabundans under high alkaline (Santos et al, 2012). The changes in the FA profile in response to high salinity and high alkaline are inevitable to keep the membrane fluid and prevent its destruction.…”

Section: Saltssupporting

confidence: 83%

NaCl as an effective inducer for lipid accumulation in freshwater microalgae Desmodesmus abundans

Xia

Rong

Yang

et al. 2014

Bioresource Technology

105

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Section: Saltssupporting

confidence: 83%

NaCl as an effective inducer for lipid accumulation in freshwater microalgae Desmodesmus abundans

Xia

Rong

Yang

et al. 2014

Bioresource Technology

105

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“…The presented method is based on the method described by Lamers et al 23 and Santos et al 24 and has also been applied by various other authors 1,[25][26][27] . Also other methods are available that are based on the same principles and could provide similar results 9,28 .…”

mentioning

confidence: 99%

Analysis of Fatty Acid Content and Composition in Microalgae

Breuer

Evers

Vree

et al. 2013

JoVE

119

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A method to determine the content and composition of total fatty acids present in microalgae is described. Fatty acids are a major constituent of microalgal biomass. These fatty acids can be present in different acyl-lipid classes. Especially the fatty acids present in triacylglycerol (TAG) are of commercial interest, because they can be used for production of transportation fuels, bulk chemicals, nutraceuticals (ω-3 fatty acids), and food commodities. To develop commercial applications, reliable analytical methods for quantification of fatty acid content and composition are needed. Microalgae are single cells surrounded by a rigid cell wall. A fatty acid analysis method should provide sufficient cell disruption to liberate all acyl lipids and the extraction procedure used should be able to extract all acyl lipid classes.With the method presented here all fatty acids present in microalgae can be accurately and reproducibly identified and quantified using small amounts of sample (5 mg) independent of their chain length, degree of unsaturation, or the lipid class they are part of. This method does not provide information about the relative abundance of different lipid classes, but can be extended to separate lipid classes from each other.The method is based on a sequence of mechanical cell disruption, solvent based lipid extraction, transesterification of fatty acids to fatty acid methyl esters (FAMEs), and quantification and identification of FAMEs using gas chromatography (GC-FID). A TAG internal standard (tripentadecanoin) is added prior to the analytical procedure to correct for losses during extraction and incomplete transesterification. Video LinkThe video component of this article can be found at

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“…These results are consistent with those of earlier researches. The growth of N. oleoabundans shows the optimum growth rate and lipid productivity in an alkaline condition (pH 8.0-9.0) in bulk-scale cultivation by Santos et al [26,27]. However, our result indicates that the growth of N. oleoabundans was inhibited by alkalinity of pH 8.0 in microscale, because the cells were easily affected by external stimulation by large surface area, permeability and dimension of microreactor compared to bulk scale [28][29][30].…”

Section: Effect Of Initial Ph In Medium For Growthmentioning

confidence: 99%

A microscale approach for simple and rapid monitoring of cell growth and lipid accumulation in Neochloris oleoabundans

Kwak¹,

Kim²,

Sim³

2015

Bioprocess Biosyst Eng

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Due to the increasing environmental problems caused by the use of fossil fuels, microalgae have been spotlighted as renewable resources to produce biomass and biofuels. Therefore, the investigation of the optimum culture conditions of microalgae in a short time is one of the important factors for improving growth and lipid productivity. Herein, we developed a PDMS-based high-throughput screening system to rapidly and easily determine the optimum conditions for high-density culture and lipid accumulation of Neochloris oleoabundans. Using the microreactor, we were able to find the optimal culture conditions of N. oleoabundans within 5 days by rapid and parallel monitoring growth and lipid induction under diverse conditions of light intensity, pH, CO2 and nitrate concentration. We found that the maximum growth rate (µ max = 2.13 day(-1)) achieved in the microreactor was 1.58-fold higher than that in a flask (µ max = 1.34 day(-1)) at the light intensity of 40 µmol photons m(-2) s(-1), 5 % CO2 (v/v), pH 7.5 and 7 mM nitrate. In addition, we observed that the accumulation of lipid in the microreactor was 1.5-fold faster than in a flask under optimum culture condition. These results show that the microscale approach has the great potential for improving growth and lipid productivity by high-throughput screening of diverse optimum conditions.

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Growth of oil accumulating microalga Neochloris oleoabundans under alkaline–saline conditions

Cited by 103 publications

References 27 publications

NaCl as an effective inducer for lipid accumulation in freshwater microalgae Desmodesmus abundans

NaCl as an effective inducer for lipid accumulation in freshwater microalgae Desmodesmus abundans

Analysis of Fatty Acid Content and Composition in Microalgae

A microscale approach for simple and rapid monitoring of cell growth and lipid accumulation in Neochloris oleoabundans

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