Growth of Stem Cell Concentrates in Diffusion Chambers (Dc)

Abstract

The growth in diffusion chamber (DC) of normal murine marrow and marrow separated by discontinuous albumin centrifugation was studied. The colony‐forming cells (CFC) assayed in soft agar, total cell counts and differentials were measured in the DC over a 19 day period after intraperitoneal implantation into CF1 mice. Growth of implants of normal marrow or fraction 3 (F3) in which CFC had been concentrated 1.7–3.9‐fold were compared at an initial cell concentration of 1 × 105. There was a good correlation between the number of CFC implanted with granulocyte production but not with macrophage production. When higher cell concentrations of normal unfractionated marrow were implanted growth was reduced as was recovery of CFC. In two experiments in which both CFU and CFC concentrations were measured there was a general correlation between the two.

Growth of Haematopoietic Cells of Mouse Fetal Liver in Diffusion Chambers

“…However, the nearly pure erythroblas tic population of the primary inoculum changed to vigorously growing granulocytic cells and macrophages during the 8-day assay period. It seems likely that end-prod uct inhibition is operative within the monocyte-macrophage and granulocyte cell li neages, respectively, when cells grow in diffusion chambers in vivo.It has been shown that intraperitoneal diffusion chambers provide a good assay system for the study of normal and malignant haematopoiesis [2,7,12,14,16]. The diffusion chamber technique seems to be particu larly well suited for the quantitative evaluation of growth of haemato poietic cells [1], Murine haematopoietic stem cells proliferate and differ entiate well in these closed in vivo cultures; granulocytes and macro phages usually dominate the cultures, whereas the occurrence of other cell lineages is more or less occasional [for a review, see B e n e s t a d and B r e i v ik , 3 ].…”