Growth of the salt-tolerant yeast in microtiter plates: effects of NaCl, pH and temperature on growth and fusel alcohol production from branched-chain amino acids

Jansen, Michael; Veurink, Janine H.; Euverink, Gert-Jan; Dijkhuizen, Lubbert

doi:10.1016/s1567-1356(02)00162-9

Cited by 14 publications

(14 citation statements)

References 14 publications

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“…Z. rouxii is an osmo-and halotolerant species used in some fermentation processes (Solieri and Giudici 2007). It is able to grow on high concentrations of salt and/or sugar ( Jansen et al 2003) and is often considered as a food-spoiling agent. The type strain (CBS732) is haploid, but some wild isolates of Z. rouxii are diploid (Solieri et al 2008).…”

Section: Rationale Of Species Selectionmentioning

confidence: 99%

Comparative genomics of protoploid Saccharomycetaceae

Souciet¹,

Dujon²,

Gaillardin³

et al. 2009

Genome Res.

193

196

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The Génolevures Consortium 1 Our knowledge of yeast genomes remains largely dominated by the extensive studies on Saccharomyces cerevisiae and the consequences of its ancestral duplication, leaving the evolution of the entire class of hemiascomycetes only partly explored. We concentrate here on five species of Saccharomycetaceae, a large subdivision of hemiascomycetes, that we call ''protoploid'' because they diverged from the S. cerevisiae lineage prior to its genome duplication. We determined the complete genome sequences of three of these species: Kluyveromyces (Lachancea) thermotolerans and Saccharomyces (Lachancea) kluyveri (two members of the newly described Lachancea clade), and Zygosaccharomyces rouxii. We included in our comparisons the previously available sequences of Kluyveromyces lactis and Ashbya (Eremothecium) gossypii. Despite their broad evolutionary range and significant individual variations in each lineage, the five protoploid Saccharomycetaceae share a core repertoire of approximately 3300 protein families and a high degree of conserved synteny. Synteny blocks were used to define gene orthology and to infer ancestors. Far from representing minimal genomes without redundancy, the five protoploid yeasts contain numerous copies of paralogous genes, either dispersed or in tandem arrays, that, altogether, constitute a third of each genome. Ancient, conserved paralogs as well as novel, lineage-specific paralogs were identified.

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Section: Rationale Of Species Selectionmentioning

confidence: 99%

Comparative genomics of protoploid Saccharomycetaceae

Souciet¹,

Dujon²,

Gaillardin³

et al. 2009

Genome Res.

193

196

View full text Add to dashboard Cite

show abstract

“…While it has a number of beneficial uses in fermentation processes (e.g. soy sauce, balsamic vinegar), it is also an osmotolerant yeast capable of growth in the presence of high concentrations of salts and sugar and is therefore one of the most serious spoilage microorganisms (Jansen et al, 2003;Pribylova et al, 2007). By allowing a significant use by Z. rouxii of the small amide compounds in food materials, the amidase gene identified in this study may contribute to this capacity to cause food spoilage.…”

Section: Discussionmentioning

confidence: 87%

The Fps1p aquaglyceroporin facilitates the use of small aliphatic amides as a nitrogen source by amidase-expressing yeasts

Shepherd

Piper

2010

FEMS Yeast Research

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Saccharomyces cerevisiae acquires a resistance to high, toxic levels of acetic acid by destabilizing Fps1p, the plasma membrane aquaglyceroporin through which this acid - in its undissociated state - enters the cell. In this study, Fps1p loss was shown to confer resistances to acetic acid, acrolein and allyl alcohol, not just in S. cerevisiae but also in the osmotolerant spoilage yeast Zygosaccharomyces rouxii. However, in Z. rouxii, the loss of Fps1p severely compromised the use of acetamide and several other small amides as sources of nitrogen, an indication that these amides enter the cells of this yeast by passive diffusion through the Fps1p pore. Saccharomyces cerevisiae cannot grow on acetamide, but was conferred with an ability to use this and other small amides as nitrogen sources by heterologous expression of a Z. rouxii ORF (ZrAMD1) with protein sequence identity to the amdS-encoded amidase of Aspergillus nidulans. This capacity of ZrAMD1-expressing S. cerevisiae to assimilate amide nitrogen was severely compromised by the loss of Fps1p. ZrAMD1 appears to encode the major amidase of Z. rouxii as a Zramd1Delta deletant mutant had, like the Zrfps1Delta deletant, lost the ability to assimilate small amides as sources of nitrogen.

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“…This device prevented contamination and allowed negligible evaporation (B/10 mL per well per day). Jansen et al (2003) also reported the use of a polypropylene mat covering a 1.2 mL nominal volume round deepwell microtiter plate, with a filling volume of 0.3 mL, working at 900 rpm with a throw of 1.5 mm, at 308C. Reeves et al (2004) covered microtiter wells with tissue-paper lined lids as to reduce evaporation and well-to-well splashing.…”

Section: Microtiter Platesmentioning

confidence: 99%

Microlitre/millilitre shaken bioreactors in fermentative and biotransformation processes – a review

Fernandes¹,

Cabral²

2006

Biocatalysis and Biotransformation

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The use of small-scale vessels (below 100 mL) as tools for the characterization of fermentative and biotransformation processes is an emergent approach, which may provide a wide array of data with significant time and cost savings. These result from the reduced reagent requirements and the possibility of simultaneously carrying out the evaluation of multiple operational parameters and assessing their individual or combined effect in the process. Furthermore, it is envisaged that such data will provide the basis for a rational scale-up of the systems evaluated. This requires an engineering approach, which has been integrated with the development of microscale technology. This review comprises recent findings related to the use and characterization of Erlenmeyer type flasks, test tubes and microtiter plates as bioreactors.

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Growth of the salt-tolerant yeast in microtiter plates: effects of NaCl, pH and temperature on growth and fusel alcohol production from branched-chain amino acids

Cited by 14 publications

References 14 publications

Comparative genomics of protoploid Saccharomycetaceae

Comparative genomics of protoploid Saccharomycetaceae

The Fps1p aquaglyceroporin facilitates the use of small aliphatic amides as a nitrogen source by amidase-expressing yeasts

Microlitre/millilitre shaken bioreactors in fermentative and biotransformation processes – a review

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