Growth pattern of experimental squamous cell carcinoma in rat submandibular glands—An immunohistochemical evaluation

Sumitomo, Shinichiro; Hashimura, Kouji; Mori, Masahiko

doi:10.1016/0964-1955(95)00080-1

Cited by 12 publications

(12 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In contrast, CK7 and CK8 mAbs showed different immunoreactivities. Similar results were published on rabbit (Farina and Zelda, 1992), human (Burns et al, 1988;Born et al, 1987;Geiger et al, 1987), and rat SSG (Sumitomo et al, 1996). It can be assumed that weak staining of mucous acinar cells is probably a cosequence of the mucin present.…”

Section: Discussionsupporting

confidence: 85%

“…These findings indicated that acinar cells (especial serous) possess CK 7,8 and 18 that are characteristic for simple epithelia. A weak positive reactivity of K8.12 mAb was found in luminal duct cells and has been identified by other investigators (Burns et al, 1988;Sumitomo et al,1996). Based on these results, it can be assumed that K8.13mAb could react with CK pair 8/18 and CK7 in acinar cells of rat SSG.…”

Section: Discussionsupporting

confidence: 78%

See 1 more Smart Citation

Immunohistochemical identification of cytokeratins in the rat submandibular salivary glands during ontogenesis

Dožić¹,

Todorović²,

Dozic³

et al. 2009

Acta vet. (Beogr.)

View full text Add to dashboard Cite

Cytokeratins (CK) are a group of proteins comprised of at least 20 different skeletal polypeptides specific for simple and complex epithelia of almost all tissues. Until recently only a few antibodies for cytoskeletal proteins were available and thus it was very difficult to visualize all the components of postnatal cytodifferentiation of salivary glands. Therefore, morphologic and immunophenotypic features of the CK polypeptides, have been analized within the rat submandibular salivary glands (SSG) during postnatal development. SSG were obtained from 1, 30 and 60 days old, male, AO rats. Streptavidin-biotin immunoperoxidase method was used for immunohistochemistry. Cryostat sections were fixed in acetone and incubated with monoclonal antibodies (mAbs) which specifically react with CK polypeptides (CK7, CK8, CK18, CK kidney, K8.12, K8.13) and rat epithelium (PT13D11). Immunohistochemical analysis of the rat SSG showed phenotypic heterogeneity of particular components of this gland during postnatal development. Regarding CK profiles it was shown that epithelial cells of the acini expressed CK18, whereas epithelial cells of the duct expressed K8.12 and K8.13 mAbs. Epithelial cells of the acini were not stained by K8.12 and PT13D11 mAbs. Intercalated ducts were strongly CK kidney+ and PT13D11+. Myoepithelial cells of the acini were stained by K8.13 mAbs, whereas these cells were not stained by mAbs specific for simple epithelia. This immunohistological study showed that the heterogeneity expression of CK polypeptides might also be useful to further understand the origin of epithelial SSG celss, as well as their development and function

show abstract

Section: Discussionsupporting

confidence: 85%

Section: Discussionsupporting

confidence: 78%

Immunohistochemical identification of cytokeratins in the rat submandibular salivary glands during ontogenesis

Dožić¹,

Todorović²,

Dozic³

et al. 2009

Acta vet. (Beogr.)

View full text Add to dashboard Cite

show abstract

“…Current concepts of salivary gland tumorigenesis require modi®cation because all cell types, including acinar cells, are at risk in the carcinogenic process (Ballagh et al, 1994). All segments of the ductual system, which undergo squamous metaplasia, may be involved in the origin of malignant tumours during experimental carcinogenesis (Sumitomo et al, 1996).…”

Section: Tumorigenesismentioning

confidence: 99%

“…Although all ductal segments are potential sites for developing experimental cancer, acinar cells do not appear to be involved in the metaplastic process (Sumitomo et al, 1996;Takai et al, 1986). The granular convoluted tubule cells are the targets in chemical submandibular gland carcinogenesis (Takai et al, 1986).…”

Section: Experimental Aspectsmentioning

confidence: 99%

Influence of environmental and nutritional factors on salivary gland tumorigenesis with a special reference to dietary lipids

Actis

Eynard

2000

Eur J Clin Nutr

View full text Add to dashboard Cite

“…Therefore, it is a widely used marker for evaluation of the proliferating cell fraction in human tumours (Bolton et al 1992;Yu et al 1992). However, only a few data were published about its expression during experimental carcinogenesis (Sumitomo et al 1996;Youssef et al 1997). However, only a few data were published about its expression during experimental carcinogenesis (Sumitomo et al 1996;Youssef et al 1997).…”

Section: Introductionmentioning

confidence: 99%

Characterisation of the progression of azaserine-induced rat pancreatic adenocarcinoma by proliferative cell nuclear antigen, basement membrane laminin and trypsinogen immunohistochemistry

Nagy

Pálfia

Réz

2003

Histochem Cell Biol

View full text Add to dashboard Cite

The progression of azaserine-induced rat pancreatic adenocarcinoma (AC) was characterised using quantitative and semiquantitative immunohistochemistry for proliferating cell nuclear antigen (PCNA), basement membrane laminin (BML) and trypsinogen (TG). Samples were taken 5-20 months after initiation. High PCNAlabelling indices (PCNA LIs) were measured 5 months after the induction of atypical acinar cell nodules (AACNs), which decreased later and stagnated until a further decline in the month 10 adenomas. Then a second premalignant proliferative wave was observed (month 13) within the adenoma stage. Later, in month 20 differentiated ACs PCNA LIs fell to the host tissue level but were found highest in the month 20 anaplastic ACs indicating a switch to malignant proliferation. Month 20 invasive ACs showed a number of separate proliferative foci. In early AACNs, BML decreased and remained low till the local maximum in the month 13 adenoma. Invasive ACs did not express BML. Month 5 AACN and differentiated AC were TG deficient but anaplastic AC regained its TG expression. However invasive AC was again TG negative. These results are discussed in combination with our previous data on progressional changes of autophagic capacity and microvessel densities.

show abstract

Growth pattern of experimental squamous cell carcinoma in rat submandibular glands—An immunohistochemical evaluation

Cited by 12 publications

References 27 publications

Immunohistochemical identification of cytokeratins in the rat submandibular salivary glands during ontogenesis

Immunohistochemical identification of cytokeratins in the rat submandibular salivary glands during ontogenesis

Influence of environmental and nutritional factors on salivary gland tumorigenesis with a special reference to dietary lipids

Characterisation of the progression of azaserine-induced rat pancreatic adenocarcinoma by proliferative cell nuclear antigen, basement membrane laminin and trypsinogen immunohistochemistry

Contact Info

Product

Resources

About