Growth performance and intestinal replacement time of 13C in newly weaned piglets supplemented with nucleotides or glutamic acid

Santos, Luan Sousa dos; Miassi, G.M.; Tse, Marcos Lívio Panhoza; Gomes, Lívea Maria; Berto, Patricia Nardin; Denadai, Juliana Célia; Caldara, Fabiana Ribeiro; Dalto, Danyel Bueno; Berto, Dirlei Antônio

doi:10.1016/j.livsci.2019.07.008

Cited by 5 publications

(6 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The delay in replacing about 50% carbon atoms in these two tissues is probably due to the low intake of food by the piglets, right after weaning. Santos et al (2019) evaluated the mucosa turnover of post-weaned piglets, and found that the incorporation of the isotopic signal from the C 3 diet only begins after the end of the critical period assigned to weaning. This critical phase is characterized by reductions in food intake and growth rate, and an increase in the occurrence of diarrhea in piglets (Rhouma, Fairbrother, Beaudry, & Letellier, 2017).…”

Section: Resultsmentioning

confidence: 99%

Isotopic carbon turnover in pig hoof and rib

Blaitt

Tierzo

Denadai

et al. 2021

Acta Sci. Anim. Sci.

View full text Add to dashboard Cite

The objective of this study was to evaluate the behavior of carbon incorporation and turnover in hoof and ribs of pigs at different periods of development in the search for tissues that reflect longer the former diet. We used 132 commercial hybrids (barrows and females), weaned at an average age of 21 days, distributed in a completely randomized design with four treatments on different days of substitution of corn (C4 cycle plant grain) diets with broken rice (C3 cycle plant grain) at 21, 42, 63 and 110 days of age to change the carbon-13 isotope signal. By means of isotopic dilution curves, we observed that animals whose C4 diet was replaced with C3 diet at 21, 42, 63 and 110 days of age, for hoof and rib, reached a new level of isotope equilibrium. Bone samples are better choices to reflect the former diet, due to conservation of the isotopic signal for longer.

show abstract

Section: Resultsmentioning

confidence: 99%

Isotopic carbon turnover in pig hoof and rib

Blaitt

Tierzo

Denadai

et al. 2021

Acta Sci. Anim. Sci.

View full text Add to dashboard Cite

show abstract

“…These results indicate that dietary GLU promotes the growth performance of pigs by increasing feed intake and performing as a metabolic fuel that more effectively increases cell turnover rate during intestinal development [ 8 , 9 ]. Moreover, it is well known that GLU has umami properties that may have an important role in the taste, palatability, and acceptability of the feeds [ 6 , 31 ]. The GLU not only stimulates taste and oral sensation by binding with umami receptors in the oral cavity, but is also involved in signaling in the brain for the autonomic reflex of the digestive responses associated with salivation for chewing and swallowing [ 11 ].…”

Section: Discussionmentioning

confidence: 99%

“…It is known that glutamic acid (GLU) is a non-essential amino acid that is abundantly present in the body and plays an essential function in cellular metabolism and immune responses [ 5 ]. The GLU is a precursor for protein synthesis and has been attracting attention for its diverse metabolic functions in weaned pigs [ 6 , 7 ]. Moreover, GLU is an important precursor for intestinal formation and immune system in the small intestine and can contribute to the immediate effect on nutrient absorption [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Dietary Glutamic Acid Modulates Immune Responses and Gut Health of Weaned Pigs

Kyoung

Lee

Cho

et al. 2021

Animals

View full text Add to dashboard Cite

Dietary glutamic acid (GLU) is used as a feed additive because of its functional characteristics that may affect the growth performance and health of pigs. This study was carried out to determine the effects of dietary GLU on growth performance, nutrient digestibility, immune responses, and intestinal health of weaned pigs. A total of ninety-six weaned pigs (8.07 ± 1.17 kg of body weight; 28 days of age) were assigned to two dietary treatments (8 pigs/pen; 6 replicates/treatment) in a randomized complete block design (block: body weight): 1) a typical weaner diet (CON) and 2) CON supplemented with 0.5% GLU. The experimental period was for 4 weeks. All data and sample collections were performed at the specific time points during the experimental period. Pigs fed GLU had higher average daily gain and average daily feed intake for the first two weeks and nutrient digestibility than pigs fed CON. In addition, dietary GLU increased villus height to crypt depth ratio, number of goblet cells, and ileal gene expression of claudin family and occludin compared with CON, but decreased serum TNF-α and IL-6 and ileal gene expression of TNF-α. Moreover, pigs fed GLU had increased relative composition of bacterial communities of genus Prevotella and Anaerovibrio and decreased genus Clostridium and Terrisporobacter compared with those fed CON. This study suggests that dietary GLU influences growth performance and health of weaned pigs by modulating nutrient digestibility, intestinal morphology, ileal gene expression of tight junction proteins and cytokines, immune responses, and microbial community in the gut.

show abstract

“…S1P is also reported to trigger glutamate secretion and potentiates depolarization-evoked glutamate secretion [ 27 ]. Glutamic acid has been found to play a crucial role in FE as it improves the FE of weaned piglets [ 28 ]. This supports the results in the current study where SGPL1 was associated with L-glutamic acid 5-phosphate as a significant gene-metabolite pair.…”

Section: Discussionmentioning

confidence: 99%

Integrative Analysis of Metabolomic and Transcriptomic Profiles Uncovers Biological Pathways of Feed Efficiency in Pigs

2020

View full text Add to dashboard Cite

Feed efficiency (FE) is an economically important trait. Thus, reliable predictors would help to reduce the production cost and provide sustainability to the pig industry. We carried out metabolome-transcriptome integration analysis on 40 purebred Duroc and Landrace uncastrated male pigs to identify potential gene-metabolite interactions and explore the molecular mechanisms underlying FE. To this end, we applied untargeted metabolomics and RNA-seq approaches to the same animals. After data quality control, we used a linear model approach to integrate the data and find significant differently correlated gene-metabolite pairs separately for the breeds (Duroc and Landrace) and FE groups (low and high FE) followed by a pathway over-representation analysis. We identified 21 and 12 significant gene-metabolite pairs for each group. The valine-leucine-isoleucine biosynthesis/degradation and arginine-proline metabolism pathways were associated with unique metabolites. The unique genes obtained from significant metabolite-gene pairs were associated with sphingolipid catabolism, multicellular organismal process, cGMP, and purine metabolic processes. While some of the genes and metabolites identified were known for their association with FE, others are novel and provide new avenues for further research. Further validation of genes, metabolites, and gene-metabolite interactions in larger cohorts will elucidate the regulatory mechanisms and pathways underlying FE.

show abstract

Growth performance and intestinal replacement time of 13C in newly weaned piglets supplemented with nucleotides or glutamic acid

Cited by 5 publications

References 30 publications

Isotopic carbon turnover in pig hoof and rib

Isotopic carbon turnover in pig hoof and rib

Dietary Glutamic Acid Modulates Immune Responses and Gut Health of Weaned Pigs

Integrative Analysis of Metabolomic and Transcriptomic Profiles Uncovers Biological Pathways of Feed Efficiency in Pigs

Contact Info

Product

Resources

About