Growth performance and nutrient utilization of growth hormone transgenic female triploid Atlantic salmon (Salmo salar) reared at three temperatures in a land-based freshwater recirculating aquaculture system (RAS)

Ignatz, Eric H.; Dumas, André; Benfey, Tillmann J.; Hori, Tiago S.; Braden, Laura M.; Runighan, C. Dawn; Rise, Matthew L.; Westcott, Jillian D.

doi:10.1016/j.aquaculture.2019.734896

Cited by 16 publications

(21 citation statements)

References 66 publications

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“…A full description of the production and rearing conditions of fish used in the current study can be found in Ignatz et al (2020b) . Briefly, all fish were from St. John River stock and were hatched and reared at AquaBounty Canada (PE, Canada).…”

Section: Methodsmentioning

confidence: 99%

“…While recommendations have been made for the optimal rearing temperature of conventional diploid Atlantic salmon ( Handeland et al, 2008 ; Hevrøy et al, 2013 ) and non-transgenic triploid Atlantic salmon ( Sambraus et al, 2017a ) in seawater, until recently, no information was available in this regard for AAS reared in freshwater RAS. It is now known what effect rearing temperature (i.e., 10.5, 13.5, and 16.5°C) has on the growth performance, nutrient utilization and innate antiviral response of AAS ( Ignatz et al, 2020a ; Ignatz et al, 2020b ). Results showed that at 16.5°C, FCR was higher, dietary lipid deposition was diverted more to the viscera than to muscle, fillet yields were lower, and ω3 fatty acid deposition was less efficient, but weight gain was higher and faster than AAS reared at 10.5 and 13.5°C ( Ignatz et al, 2020b ).…”

Section: Introductionmentioning

confidence: 99%

“…It is now known what effect rearing temperature (i.e., 10.5, 13.5, and 16.5°C) has on the growth performance, nutrient utilization and innate antiviral response of AAS ( Ignatz et al, 2020a ; Ignatz et al, 2020b ). Results showed that at 16.5°C, FCR was higher, dietary lipid deposition was diverted more to the viscera than to muscle, fillet yields were lower, and ω3 fatty acid deposition was less efficient, but weight gain was higher and faster than AAS reared at 10.5 and 13.5°C ( Ignatz et al, 2020b ). Further, when intraperitoneally injected with either polyriboinosinic polyribocytidylic acid [pIC; a synthetic double-stranded RNA (dsRNA) analog that elicits a potent antiviral-like response] or an equal volume of sterile phosphate-buffered saline (PBS), higher induction of target antiviral biomarker transcripts was generally found at 10.5°C than either 13.5 or 16.5°C ( Ignatz et al, 2020a ).…”

Section: Introductionmentioning

confidence: 99%

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RNA-Seq Analysis of the Growth Hormone Transgenic Female Triploid Atlantic Salmon (Salmo salar) Hepatic Transcriptome Reveals Broad Temperature-Mediated Effects on Metabolism and Other Biological Processes

Ignatz

Hori²,

Kumar

et al. 2022

Front. Genet.

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This study examined the impact of rearing temperature (10.5, 13.5 or 16.5°C) on the hepatic transcriptome of AquAdvantage Salmon (growth hormone transgenic female triploid Atlantic salmon) at an average weight of 800 g. Six stranded PE libraries were Illumina-sequenced from each temperature group, resulting in an average of over 100 M raw reads per individual fish. RNA-sequencing (RNA-seq) results showed the greatest difference in the number of differentially expressed transcripts (1750 DETs), as revealed by both DESeq2 and edgeR (q < 0.05; fold-change > |1.5|), was between the 10.5 and 16.5°C temperature groups. In contrast, 172 and 52 DETs were found in the 10.5 vs. 13.5°C and the 13.5 vs. 16.5°C comparisons, respectively. Considering the DETs between the 10.5 and 16.5°C groups, 282 enriched gene ontology (GO) terms were identified (q < 0.05), including “response to stress”, “immune system process”, “lipid metabolic process”, “oxidation-reduction process”, and “cholesterol metabolic process”, suggesting elevated temperature elicited broad effects on multiple biological systems. Pathway analysis using ClueGO showed additional impacts on amino acid and lipid metabolism. There was a significant positive correlation between RNA-seq and real-time quantitative polymerase chain reaction (RT-qPCR) results for 8 of 9 metabolic-related transcripts tested. RT-qPCR results also correlated to changes in fillet tissue composition previously reported in these salmon (e.g., methionine and lysine concentrations positively correlated with hsp90ab1 transcript expression), suggesting that rearing temperature played a significant role in mediating metabolic/biosynthetic pathways of AquAdvantage Salmon. Many transcripts related to lipid/fatty acid metabolism (e.g., elovl2, fabpi, hacd2, mgll, s27a2, thrsp) were downregulated at 16.5°C compared to both other temperature groups. Additionally, enrichment of stress-, apoptosis- and catabolism-relevant GO terms at 16.5°C suggests that this temperature may not be ideal for commercial production when using freshwater recirculating aquaculture systems (RAS). This study relates phenotypic responses to transcript-specific findings and therefore aids in the determination of an optimal rearing temperature for AquAdvantage Salmon. With approval to grow and sell AquAdvantage Salmon in the United States and Canada, the novel insights provided by this research can help industry expansion by promoting optimal physiological performance and health.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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RNA-Seq Analysis of the Growth Hormone Transgenic Female Triploid Atlantic Salmon (Salmo salar) Hepatic Transcriptome Reveals Broad Temperature-Mediated Effects on Metabolism and Other Biological Processes

Ignatz

Hori²,

Kumar

et al. 2022

Front. Genet.

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“…Approximately 10 μL of this solution was added to 70 μL of AccQ-Fluor Borate and 20 μL of Fluor A reagent, and total mixtures were vortexed for 1 min. Finally, solutions were incubated at 55°C for 10 min before being injected into the UPLC system (Rosmawati et al 2018;Ignatz et al 2020).…”

Section: Growth Performance and Amino Acid And Protein Content Of G 3mentioning

confidence: 99%

Growth hormone transgenesis and feed composition influence growth and protein and amino acid content in transgenic G3 mutiara catfish (Clarias gariepinus)

2021

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Clarias gariepinus growth hormone (CgGH) transgenesis was previously used to develop a population of second-generation (G2) transgenic mutiara catfish (C. gariepinus). The third generation of these fish (1-month old fingerlings) had 2–3 times improvement in growth compared to non-transgenic fish in the commercial feed test for 6 weeks of rearing. We assessed the impact of CgGH transgene expression on growth and protein and amino acid content of the G3 generation of these transgenic mutiara catfish relative to non-transgenic catfish. Since variation in composition of feed mixes can affect protein and amino acid content of fish, we tested three mixtures of commercial feed and boiled tuna (Euthynnus affinis): feed A (50:50 feed to tuna), B (65:35), and C (80:20) to transgenic catfish. Feed A* (50:50) was fed as a control to non-transgenic catfish. Feed efficiency, including feed conversion ratio and protein use efficiency (i.e., protein retention and protein productive value), was assessed. Feed efficiency, protein content, and essential amino acid content in G3 transgenic catfish (feed A and B) were higher than in non-transgenic fish (feed A*). The latter were deficient in lysine and methionine. Transgenic catfish fed with feed C (80:20) showed lysine deficiency and lower growth than fish fed feeds A and B. Feed B (65:35) was the optimal feed mixture utilized; it increased growth, protein levels, and feed conversion efficiency in G3 catfish. The growth of transgenic fish was higher than non-transgenic fish when supported by feeding with balanced nutrients.

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“…As example, the transgeniccommercial AquAdvantage ® salmon are produced as triploid females, which do not contain fertile gametes and thus have little reproductive potential. They are therefore incompatible with wild diploid fish (Ignatz et al, 2020).…”

Section: Transgene Transmissionmentioning

confidence: 99%

Gene Transmission, Growth, and Exogeneous Growth Hormone Expression of G2 Transgenic Betta Fish (Betta imbellis)

Ayuningthias

Nasrullah

Soelistyowati

et al. 2021

JIPK

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Highlight ResearchThe F2 of GH-transgenic B. imbellis was successfully producedThe transgene inheritance by the F2 fish was more than 90%The growth and body size of transgenic fish was significantly higher than controlF2 fish reached a larger body size in a shorter period compared to the F1 AbstractIn our previous research, we had successfully produced G0 and G1 Pangasianodon hypophthalmus growth hormone (PhGH) transgenic B. imbellis, native ornamental betta from Indonesia, which its giant-sized variant has valuable price for the breeders. The G0 and G1 transgenic (TG) fish showed higher growth rate and body size compared to the non-transgenic (NT) fish. The study was aimed to produce and evaluate the consistencies of transgene transmission and expression in G2 generation. The growth rate and body size between TG and NT fish was also compared. The G2 generation was produced using crosses between TG and NT G1 fish: ♂TG × ♀TG, ♂TG × ♀NT, ♂NT × ♀TG, and ♂NT ×♀ NT. Fish were reared for 12 weeks, and transgene detection was performed using the polymerase chain reaction method (PCR) on isolated DNA from the caudal fin clips. The endogenous and exogenous GH expression analysis was conducted using the quantitative real-time PCR (qPCR) method. The results showed that the inheritance of the GH transgene by the G2 fish was more than 90% in all transgenic crosses. Endogenous GH was expressed at the same levels in the brain of TG and NT fish, but the exogenous GH expression was highly detected only in the TG fish. The G2 transgenic fish had a higher specific growth rate, up to 31%, compared to the control. The body length of TG crosses were 23−35% higher and had 111−135% higher body weight compared to NT fish. These results showed a promising approached in mass-producing stable lines of giant-sized betta using the GH-transgenic technology.

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Growth performance and nutrient utilization of growth hormone transgenic female triploid Atlantic salmon (Salmo salar) reared at three temperatures in a land-based freshwater recirculating aquaculture system (RAS)

Cited by 16 publications

References 66 publications

RNA-Seq Analysis of the Growth Hormone Transgenic Female Triploid Atlantic Salmon (Salmo salar) Hepatic Transcriptome Reveals Broad Temperature-Mediated Effects on Metabolism and Other Biological Processes

RNA-Seq Analysis of the Growth Hormone Transgenic Female Triploid Atlantic Salmon (Salmo salar) Hepatic Transcriptome Reveals Broad Temperature-Mediated Effects on Metabolism and Other Biological Processes

Growth hormone transgenesis and feed composition influence growth and protein and amino acid content in transgenic G3 mutiara catfish (Clarias gariepinus)

Gene Transmission, Growth, and Exogeneous Growth Hormone Expression of G2 Transgenic Betta Fish (Betta imbellis)

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