Growth-promoting and antimetamorphic hormone in pituitary glands of bullfrogs

Kikuyama, Sakaé; Yamamoto, Kazutoshi; Mayumi, Masako

doi:10.1016/0016-6480(80)90145-8

Cited by 18 publications

(2 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…GH mRNA continues to decline at a time when PRL mRNA increases -5-fold. Both TSHa and -/3 mRNAs return to their control levels by day 5 (Fig. 3A).…”

mentioning

confidence: 99%

“…The experimental evidence leading to this conclusion is as follows: (i) anti-TH effects are caused by treating metamorphosing tadpoles with large doses of mammalian PRL and amphibian pituitary fractions containing PRL activity (5) but not with growth hormone (GH) (3,6,7) and (ii) the injection of antibodies to PRL in tadpoles stimulates metamorphosis (7,8). However, there is no biological evidence for the existence of a juvenilizing hormone in amphibians, and reported levels of PRL in tadpoles do not correlate with the expectation that the hormone exerts a juvenilizing effect (9).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Expression of the Xenopus laevis prolactin and thyrotropin genes during metamorphosis.

Buckbinder

Brown

1993

Proc. Natl. Acad. Sci. U.S.A.

113

View full text Add to dashboard Cite

The cDNAs encoding Xenopus laevis prolac- (3,6,7) and (ii) the injection of antibodies to PRL in tadpoles stimulates metamorphosis (7,8). However, there is no biological evidence for the existence of a juvenilizing hormone in amphibians, and reported levels of PRL in tadpoles do not correlate with the expectation that the hormone exerts a juvenilizing effect (9). This conclusion was also reached by Takahashi et al. (10), who cloned bullfrog PRL cDNA and demonstrated an increase in PRL mRNA late in metamorphosis.To study these events in greater detail, we have cloned cDNAs encoding TSH a and ,B subunits (TSHa and -,B, respectively) and PRL from aXenopus laevis pituitary cDNA library. Using these homologous probes, we describe the levels of their mRNAs during metamorphosis. We conclude that, although TSHa and -p mRNAs follow the expected developmental profile of unrestrained synthesis until just before metamorphic climax, this is not so for pituitary PRL mRNA. In fact, our results indicate that TH induces, although indirectly, the synthesis of PRL mRNA during metamorphosis.

show abstract

“…GH mRNA continues to decline at a time when PRL mRNA increases -5-fold. Both TSHa and -/3 mRNAs return to their control levels by day 5 (Fig. 3A).…”

mentioning

confidence: 99%