Growth-Promoting Potential and Toxicity of Spermidine, a Polyamine and Biogenic Amine Found in Foods and Feedstuffs

Smith, T. K.; Mogridge, Jo-Ann L.; Sousadias, M G

doi:10.1021/jf9503974

Cited by 24 publications

(13 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It seems that the C5 diamine (cadaverine) is more toxic to living organisms than the C4 diamine (putrescine). The toxicity of an amine compound depends on its aliphatic chain length and the number of free amino groups as shown in bacteria and animals (Bachrach and Weinstein, 1970; Smith et al, 1996). Further study is needed to understand the exact mechanism of the toxicity of diamine to the cell.…”

Section: Resultsmentioning

confidence: 99%

Metabolic engineering of Escherichia coli for the production of cadaverine: A five carbon diamine

Qian

Xia

Lee

2010

Biotech & Bioengineering

205

158

View full text Add to dashboard Cite

A five carbon linear chain diamine, cadaverine (1,5-diaminopentane), is an important platform chemical having many applications in chemical industry. Bio-based production of cadaverine from renewable feedstock is a promising and sustainable alternative to the petroleum-based chemical synthesis. Here, we report development of a metabolically engineered strain of Escherichia coli that overproduces cadaverine in glucose mineral salts medium. First, cadaverine degradation and utilization pathways were inactivated. Next, L-lysine decarboxylase, which converts L-lysine directly to cadaverine, was amplified by plasmid-based overexpression of the cadA gene under the strong tac promoter. Furthermore, the L-lysine biosynthetic pool was increased by the overexpression of the dapA gene encoding dihydrodipicolinate synthase through the replacement of the native promoter with the strong trc promoter in the genome. The final engineered strain was able to produce 9.61 g L(-1) of cadaverine with a productivity of 0.32 g L(-1) h(-1) by fed-batch cultivation. The strategy reported here should be useful for the bio-based production of cadaverine from renewable resources.

show abstract

Section: Resultsmentioning

confidence: 99%

Metabolic engineering of Escherichia coli for the production of cadaverine: A five carbon diamine

Qian

Xia

Lee

2010

Biotech & Bioengineering

205

158

View full text Add to dashboard Cite

show abstract

“…1998). Sousadias and Smith (1995) and Smith, Mogridge and Sousadias (1996), however, observed that these positive effects decline with increasing molecular weight and cationic charge of the polyamine molecule. Some studies with rainbow trout Oncorhyncchus mykiss (Fairgrieve, Myers, Hardy & Dong 1994) and Atlantic salmon Salmo salar L. (Opstvedt et al .…”

Section: Discussionmentioning

confidence: 95%

Response of blue shrimp Litopenaeus stylirostris (Perez-Farfante & Kensley, 1997) to dietary cadaverine supplementation

et al. 2004

View full text Add to dashboard Cite

Recent studies on salmon and shrimp have shown that reduced feed intake and growth caused by the consumption of low-quality ¢sh meals, manufactured from spoiled ¢sh, were not due to the presence of biogenic amines. Moreover, an improvement in weight gain was seen in blue shrimp fed a diet supplemented with cadaverine plus histamine. It was not clear, however, if this e¡ect was due to the consumption of histamine or cadaverine. The objective of the current experiment was to investigate the e¡ect of dietary cadaverine supplementation on growth parameters and various amine concentrations in tissues of the blue shrimp Litopenaeus stylirostris. Six experimental diets were supplemented with cadaverine at 0, 500, 1100, 2300, 3500 and 4600 mg kg À1 and tested in a feeding trial lasting 28 days. Feed consumption, feed conversion ratio, survival and weight gain were not a¡ected by the concentration of dietary cadaverine. The dietary supplementation of cadaverine, however, resulted in a linear increase in cadaverine concentration in shrimp tissues, especially in the hepatopancreas. It was concluded that dietary cadaverine does not have any e¡ect on growth and feed intake of shrimp. Growth promotion, as reported previously in shrimp fed a diet supplemented with histamine plus cadaverine, was probably due to histamine or a combined e¡ect of histamine plus cadaverine but not due to dietary cadaverine alone. It seems that shrimp have a limited ability to metabolize cadaverine, which then accumulates intact in shrimp tissues.

show abstract

“…The higher rate of mortality compared to inhibition of MMS suggests the limited effects observed for spermidine against C. savignyi likely result from general toxicity rather than inhibition of metamorphosis. Spermidine has been previously reported as toxic to week-old chicks at 40.6% of feed (Smith et al 1996) and to rats at 6.0 6 10 5 mg kg 71 (Til et al 1997). Additionally, spermidine is cytotoxic to E. coli at 700.0 mg ml 71 (Limsuwun and Jones 2000) and to baby hamster kidney cells at 1.7 mg ml 71 (Brunton et al 1991).…”

Section: Discussionmentioning

confidence: 98%

Preventing ascidian fouling in aquaculture: screening selected allelochemicals for anti-metamorphic properties in ascidian larvae

et al. 2012

View full text Add to dashboard Cite

Fouling by ascidians causes major stock losses and disrupts production in marine aquaculture, especially bivalve aquaculture. Currently, no cost effective solution exists despite the testing of many prospective control techniques. This study examined a range of allelochemicals suspected to inhibit metamorphosis in marine larvae. Five allelochemicals were screened in a larval metamorphosis bioassay using Ciona savignyi Herdman to determine their potential as a remedy for ascidian fouling in bivalve aquaculture. Three of the compounds tested inhibited ascidian larval metamorphosis and increased mortality at low concentrations. These were radicicol (99% inhibition of metamorphosis [IC₉₉], 0.8 μg ml⁻¹; 99% lethal concentration [LC₉₉], 2.5 μg ml⁻¹; 99% lethal time [LT₉₉], 7.0 days), polygodial (IC₉₉, 0.003 μg ml⁻¹; LC₉₉, 0.9 μg ml⁻¹; LT₉₉, 6.4 days), and ubiquinone-10 (IC₉₉, 3.2 μg cm⁻²; LC₉₉, 14.5 μg cm⁻²; LT₉₉, 5.6 days; expressed as μg cm⁻² due to insolubility in water and ethanol). While spermidine significantly affected metamorphosis and mortality of C. savignyi, the effect was insufficient to achieve inhibition in 99% of larvae over the 7-day timeframe of the assay. Muscimol did not affect metamorphosis or mortality at the concentrations tested. The present study demonstrates that radicicol, polygodial and ubiquinone-10 have potential for future development in antifoulant formulations targeted towards the inhibition of metamorphosis in ascidian larvae, while spermidine and muscimol appear unsuitable.

show abstract

Growth-Promoting Potential and Toxicity of Spermidine, a Polyamine and Biogenic Amine Found in Foods and Feedstuffs

Cited by 24 publications

References 13 publications

Metabolic engineering of Escherichia coli for the production of cadaverine: A five carbon diamine

Metabolic engineering of Escherichia coli for the production of cadaverine: A five carbon diamine

Response of blue shrimp Litopenaeus stylirostris (Perez-Farfante & Kensley, 1997) to dietary cadaverine supplementation

Preventing ascidian fouling in aquaculture: screening selected allelochemicals for anti-metamorphic properties in ascidian larvae

Contact Info

Product

Resources

About