Growth promotion of Suillus luteus by adenosine in vitro

Abstract

In this work, adenosine has been shown to stimulate Suillus luteus mycelial growth, branching, and aggregates on solid substrate. Adenosine promoted S. luteus mycelial growth and branching, and the fungus responded significantly at a concentration as low as 0.01 mg/ml. The highest mycelial biomass and density of three strains were observed at 0.16 mg/ml in SNP-20, 0.08 mg/ml in SAF-501, and 0.16 mg/ml in PT-501. Adenosine enhanced mycelial aggregate at concentration of 0.02 mg/ml in SNP-20. The study suggests that adenosine promotes S. luteus mycelial growth, branching, and aggregation. Thus, it may be a good candidate as a biological elicitor of mycelial growth for S. luteus.

Organic Nitrogen Supplementation Increases Vegetative and Reproductive Biomass in a Versatile White Rot Fungus

“…Still, Domondon et al [ 10 ] noted that P. pulmonarius formed dense mycelial cords (rhizopmorphs) on β-adenosine-supplemented plates. If dry weight was assessed, cord formation by this fungus upon β-adenosine supplementation might eventually reveal an increase of mycelial biomass, as detected in the present case (see Figure 1 B) and in the ectomycorrhizal (ECM) fungus Suillus luteus [ 32 ]. Other than Zhang et al [ 32 ], where 32 µg/mL β-adenosine already led to a dry weight drop over 16 µg/mL, here such an ‘overdosage effect’ was not observed when increasing the amount of β-adenosine supplementation 10×, i.e., to 150 µg/mL (see Figure 1 B).…”

“…In previous work [ 10 , 32 ] where β-adenosine or β-adenosine-/nitrogen-rich rye grass material/extract was used as a nitrogen additive, only a partial traversal of the mushroom life cycle on agar medium took place. In agreement with these studies, mycelial biomass increased both when adding 15 µg/mL or 150 µg/mL β-adenosine to the agar medium was detected here (see Figure 1 B).…”

“…In plates with 15 µg/mL β-adenosine supplementation, primordia arrived after circa nine days with the difference to the control being not significant. Albeit stimulated by orchestrated fruiting cues, S. luteus mycelia did not form any fruiting body development stages, regardless of β-adenosine addition in the agar plate-based experimental setup of [ 32 ]. Most likely, this is caused by the absence of an ectomycorrhizal partner tree, the presence of which is usually required to trigger fruiting in ECM fungi, under semi-minimalistic laboratory conditions [ 33 ].…”

Gamma-aminobutyric acid (GABA) increases in vitro germ-tube formation and phospholipase B1 mRNA expression in Candida albicans

“…The neurotransmitter serotonin attenuates in vitro C. albicans virulence (Mayr et al 2005) whereas dopamine inhibits its respiratory growth and survival (Macreadie et al 2010). In other fungi, the adenosine neurotransmitter stimulates the mycelial growth of Suillus luteus (Zhang et al 2010), and gammaaminobutyric acid (GABA) increases in vitro growth of Cladosporium fulvum (Solomon and Oliver 2002).…”

Gamma-aminobutyric acid (GABA) increases in vitro germ-tube formation and phospholipase B1 mRNA expression in <i>Candida albicans</i>

“…The neurotransmitter serotonin attenuates in vitro C. albicans virulence (Mayr et al 2005) whereas dopamine inhibits its respiratory growth and survival (Macreadie et al 2010). In other fungi, the adenosine neurotransmitter stimulates the mycelial growth of Suillus luteus (Zhang et al 2010), and gammaaminobutyric acid (GABA) increases in vitro growth of Cladosporium fulvum (Solomon and Oliver 2002).…”