Growth Regulation and Validation of Homogeneity in In Vitro-Derived Bleeding Heart by Molecular Markers and Spectral Analysis of Pigments

Abstract

The aim of this study is to analyze the morphogenetic events in Lamprocapnos spectabilis (L.) Fukuhara (bleeding heart) 'Gold Heart' and 'White Gold' micropropagated in various culture media, and to evaluate the biochemical and genetic stability of the produced plants. None of the sixteen tested plant growth regulator (PGR) combinations improved the micropropagation efficiency or morphological traits of shoots in 'Gold Heart' compared to the PGRs-free control, indicating low intrinsic regenerability of this cultivar. As for bleeding heart 'White Gold', culture medium with 0.5 mg•L −1 IBA and 2.0 mg•L −1 BA was optimal in terms of multiplication ratio (9.8) and shoot parameters. Addition of PGRs was necessary for effective rooting in both cultivars studied. Some experimental combinations declined the concentration of chlorophylls and carotenoids in the shoots of bleeding heart, especially if a significant amount of callus was formed at the shoot base. The PGR-supplemented media stimulated the abundant secretion of phenols by shoots. The survival rate and quality of the plantlets after acclimatization varied from 0.0 to 100%, depending on the composition of the multiplication medium. Plants with a higher number of leaves were more likely to survive acclimatization. Somaclonal variation was detected in 36.8 to even 69.1% of in vitro-derived plants by two genetic marker systems. Most of that variation was irrelevant, however, some genotypes were significantly different from the predominant control.

Micro‐morpho‐anatomical mechanisms involve in epiphytic adaptation of micropropagated plants of Vanda tessellata (Roxb.) Hook. ex G. Don

“…The progressive developments that occurred in the leaf anatomy provide mechanical strength to the plant and guard the leaf against desiccation (Khasim & Ramesh, 2010). As acclimatization is a non‐autonomic process, it depends on various physical parameters of the surrounding environments (Kulus, de Dieu Muhire, & Aksoy, 2020; Teixeira da Silva et al, 2017). The development of photosynthetic organs of the leaves regulates the acclimation process (Im & Grossman, 2002; Oguchi, Hikosaka, & Hirose, 2003).…”

Assessment of antibacterial activity and cytotoxic effects of in vitro and in vivo plant parts of a medicinal plant Gynura procumbens (Lour.) Merr.

“…In vitro regeneration is an effective technique for producing disease-free and genetically accurate plants [ 14 ]. However, as numerous factors, such as the type of explants, combination of plant growth regulators on media, culturing parameters, genotype, etc., influence the in vitro regeneration of plants, their biochemical properties may differ from those of in vivo materials [ 29 , 36 , 37 ].…”