In vitro propagation and cryopreservation of the medicinal species Hovenia dulcis Thunb. (Rhamnaceae)

Saavedra, Aline Medeiros; Castro, Tatiana Carvalho de; Cordeiro, Lívia da Silva; Athayde, Thaís; Albarello, Norma; Simões-Gurgel, Claudia

doi:10.1007/s11240-020-01980-6

Cited by 10 publications

(1 citation statement)

References 79 publications

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“…Also, PTC can be used for in situ and ex situ conservation, propagation, polyploidy or aneuploidy induction, plant engineering, and bioreactor applications. In vitro multiplication was established in many threatened and endemic medicinal plants, such as Bacopa monnieri [34], Paedaria foetida [35], Picrorhiza kuroa [36], Salvadora persica [37], Potentilla fulgens [38], Eryngium foetidum [39], and H. dulcis [40].…”

Section: Application Of In Vitro Culture Techniques On Medicinal Plantsmentioning

confidence: 99%

Application of Tissue Culture Techniques to Improve the Productivity of Medicinal Secondary Products from Medicinal Plants

Hassanein¹

2023

Case Studies of Breeding Strategies in Major Plant Species

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The plant kingdom is considered the most important source of medicinal chemicals. In vitro culture techniques are being considered a promising alternative to traditional agricultural processes to improve medicinal plants multiplication and their production of pharmaceutical compounds. In this chapter, several in vitro culture strategies are discussed to improve secondary metabolites production, including (1) plant kingdom as a source of medicinal chemicals, (2) in vitro culture of medicinal plants, (3) culture media optimization, (4) application of suspension cell culture for production of secondary metabolites, (5) elicitation to enhance the productivity of the culture, (6) precursor intermediates feeding, (7) selection of high-yielding cell lines, (8) overexpression of genes that control the production of bioactive compounds, and (9) scale-up production. Also, challenges that hinder the in vitro culture of medicinal plants using different techniques and the use of those techniques to produce pharmaceutical compounds are discussed in this chapter, including (a) secondary metabolites toxicity, (b) low growth rate, (c) culture browning, (d) limitation in the application of transformation, (e) somaclonal variation, and (f) vitrification. Therefore, the principal objective of the current chapter was to shed light on the studies on some medicinal plants and the used protocols to overcome some difficulties in terms of in vitro propagation that maximize their economic values.

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Section: Application Of In Vitro Culture Techniques On Medicinal Plantsmentioning

confidence: 99%

Application of Tissue Culture Techniques to Improve the Productivity of Medicinal Secondary Products from Medicinal Plants

Hassanein¹

2023

Case Studies of Breeding Strategies in Major Plant Species

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Long- and Medium-Term Storage of Germplasm for Conservation of Tree Species

Yadav,

Priya,

Dhiman

et al. 2024

Biotechnological Approaches for Sustaining Forest Trees and Their Products

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High-frequency shoot regeneration from flower bud derived callus of Gymnostachyum febrifugum Benth., an endemic medicinal plant to the Western Ghats

Silpa

Thomas

2021

Plant Cell Tiss Organ Cult

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Gymnostachyum febrifugum Benth. is a small, scapigerous, rare and endemic medicinal herb indigenous to India belonging to the family Acanthaceae. This study reports an e cient protocol for high-frequency ower bud derived callus induction and shoot organogenesis in G. febrifugum. Flower buds at 7d before anthesis (dBA) were excised from the in orescence and cultured on MS medium supplemented with various concentrations of 2, 4-dichlorophenoxy acetic acid (2, 4-D; 0.5-2.0 mg/l) for callus induction. The optimum callus induction (78%) was obtained on MS medium supplemented with 1.5 mg/l 2, 4-D. The calli when subcultured on MS medium supplemented with different concentrations of thidiazuron (TDZ; 0.5-2.5 mg/l) or 6-benzylaminopurine BAP (0.5-2.5 mg/l) alone or in combination with 1naphthaleneacetic acid (NAA; 0.2-0.7 mg/l) induced shoots. The highest frequency (94%) and number of shoots (44.6 shoots/unit callus) were obtained on MS medium supplemented with 2.0 mg/l TDZ and 0.5 mg/l NAA. The optimum rooting frequency (95%) and number of roots (10.2) were observed on ½ MS medium supplemented with 3.0 mg/l indole-3-butyric acid (IBA). The rooted plantlets were acclimatized and transferred to soil with 94% success.

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In vitro propagation and cryopreservation of the medicinal species Hovenia dulcis Thunb. (Rhamnaceae)

Cited by 10 publications

References 79 publications

Application of Tissue Culture Techniques to Improve the Productivity of Medicinal Secondary Products from Medicinal Plants

Application of Tissue Culture Techniques to Improve the Productivity of Medicinal Secondary Products from Medicinal Plants

Long- and Medium-Term Storage of Germplasm for Conservation of Tree Species

High-frequency shoot regeneration from flower bud derived callus of Gymnostachyum febrifugum Benth., an endemic medicinal plant to the Western Ghats

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