Growth regulators and darkness increase efficiency in in vitro culture of immature embryos from peppers

Manzur, Juan Pablo; Calvache-Asensio, María de las Nieves; Rodríguez‐Burruezo, Adrián

doi:10.1590/0103-9016-2013-0230

Cited by 9 publications

(2 citation statements)

References 21 publications

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“…Hasanah et al (2014) reported that banana extract contains 0.00035% IAA (auxin) and 0.00020% cytokinin. Furthermore, the action of auxin and cytokinin was influenced by light, where both hormones were degraded when exposed to light (Manzur et al 2014). This might explain the higher values of plant height and leaf length in medium NP+100 g/l banana extract in the dark than in light conditions.…”

Section: Resultsmentioning

confidence: 99%

In Vitro Culture of Phalaenopsis amabilis (L.) Blume Orchid for Seedling Production with Banana Extract Supplementation and Light Treatment for Ex Situ Conservation

Arum

Semiarti

2022

J.Tropical Biodiversity Biotechnology

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In vitro culture is one of the effective cultivation methods for seedling production of orchids that can be used as a powerful tool in the conversation of orchids. The aims of the present study were to (i) investigate the effects of the addition of banana extract (0 g/l, 100 g/l, and 150 g/l) on media (NP media), in two different light regimes (light and dark conditions) on the growth of plantlets of an epiphytic orchid Phalaenopsis amabilis in in vitro culture. Methods used included (i) subculturing orchid seedlings in treatments media, (ii) measuring leaves and roots chlorophyll content and growth parameters, (iii) anatomical preparation of leaves and roots of the seedlings. The results showed that the best condition for getting greater seedlings of P. amabilis plantlets is in media with an addition of 100 g/L banana extract in light condition. The highest amount of chlorophyll in the P. amabilis leaves was found in medium with the addition of 100 g/L banana extract medium in light conditions. The thickness of mesophyll and the largest root diameter of P. amabilis seedlings were also found in media with the addition of 100 g/L banana extract medium in light condition. In conclusion, the addition of 100gr/L banana extract into basic culture medium will be beneficial for seedlings production of P. amabilis with great appearance, for ex situ orchid conservation programs.

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Section: Resultsmentioning

confidence: 99%

In Vitro Culture of Phalaenopsis amabilis (L.) Blume Orchid for Seedling Production with Banana Extract Supplementation and Light Treatment for Ex Situ Conservation

Arum

Semiarti

2022

J.Tropical Biodiversity Biotechnology

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“…The amniotic cavity represents the internal cavity of three cavities to ensure the darkness that surrounds the early stages of embryonic development. In this regard, [2] found in bola embryos that initial dark incubation improvs efficiency rates at all embryo stages, especially in first (globular) embryos which could increase more than 6 times. It has been suggested that light could affect embryonic development in some species either by direct toxic effect on the embryo, or indirectly by photo-oxidation of components in media [3].…”

Section: Introductionmentioning

confidence: 90%

A look at early human embryonic development and the importance of darkness for embryogenesis

Hegazy

2022

Int J Cadaver Stud Ant Var

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Human development is a highly complex process where a single cell called zygote forms a multicellular body made up of trillions of cells. Human pregnancy takes about 10 lunar months or 40 weeks. Prenatal life is divided into three stages namely germinal, embryonic and fetal stages. All stages are critical, but the most important is the embryonic stage through which embryogenesis occurs. It normally occurs in the upper segment of the uterus into its endometrium, called decidua after occurrence of conception. Many factors can affect embryogenesis including the mother's use of medications and even the mother's psychological state. Of these factors, light may be. The embryo is surrounded by three regions of darkness These areas from the inside out are the amniotic cavity, the uterine cavity, and the abdominal cavity. Light could affect embryonic development either by direct toxic effect on the embryo, or indirectly by photo-oxidation of components in media. Therefore, it is necessary to study them in future studies to investigate the potentially harmful effect of light exposure on embryonic development. This may be useful to avoid potential obstacles to the IVF process as embryos can be exposed to light sources of varying intensity.

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In Vitro Embryo Rescue Techniques and Applications in Hybrid Plant Development

Amiteye

2023

Advanced Crop Improvement, Volume 2

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Growth regulators and darkness increase efficiency in in vitro culture of immature embryos from peppers

Cited by 9 publications

References 21 publications

<i>In Vitro</i> Culture of <i>Phalaenopsis amabilis</i> (L.) Blume Orchid for Seedling Production with Banana Extract Supplementation and Light Treatment for <i>Ex Situ</i> Conservation

<i>In Vitro</i> Culture of <i>Phalaenopsis amabilis</i> (L.) Blume Orchid for Seedling Production with Banana Extract Supplementation and Light Treatment for <i>Ex Situ</i> Conservation

A look at early human embryonic development and the importance of darkness for embryogenesis

In Vitro Embryo Rescue Techniques and Applications in Hybrid Plant Development

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