GsERF1 enhances Arabidopsis thaliana aluminum tolerance through an ethylene-mediated pathway

Li, Lü; Li, Xingang; Yang, Ce; Cheng, Yanbo; Cai, Zhandong; Nian, Hai; Ma, Qibin

doi:10.1186/s12870-022-03625-6

Cited by 12 publications

(11 citation statements)

References 62 publications

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“…ERF-VII Pop_ERFB2-1 could regulate the expression of hypoxia-responsive genes under high intracellular Zn concentrations. Li L. et al. (2022) suggested that overexpression of GsERF1 may enhance aluminum tolerance of Arabidopsis through an ethylene-mediated pathway and/or ABA signaling pathway.…”

Section: Erfs Regulate Plant Responses To Various Abiotic Stressesmentioning

confidence: 99%

ERF subfamily transcription factors and their function in plant responses to abiotic stresses

Zhang

et al. 2022

Front. Plant Sci.

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Ethylene Responsive Factor (ERF) subfamily comprise the largest number of proteins in the plant AP2/ERF superfamily, and have been most extensively studied on the biological functions. Members of this subfamily have been proven to regulate plant resistances to various abiotic stresses, such as drought, salinity, chilling and some other adversities. Under these stresses, ERFs are usually activated by mitogen-activated protein kinase induced phosphorylation or escape from ubiquitin-ligase enzymes, and then form complex with nucleic proteins before binding to cis-element in promoter regions of stress responsive genes. In this review, we will discuss the phylogenetic relationships among the ERF subfamily proteins, summarize molecular mechanism how the transcriptional activity of ERFs been regulated and how ERFs of different subgroup regulate the transcription of stress responsive genes, such as high-affinity K+ transporter gene PalHKT1;2, reactive oxygen species related genes LcLTP, LcPrx, and LcRP, flavonoids synthesis related genes FtF3H and LhMYBSPLATTER, etc. Though increasing researches demonstrate that ERFs are involved in various abiotic stresses, very few interact proteins and target genes of them have been comprehensively annotated. Hence, future research prospects are described on the mechanisms of how stress signals been transited to ERFs and how ERFs regulate the transcriptional expression of stress responsive genes.

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Section: Erfs Regulate Plant Responses To Various Abiotic Stressesmentioning

confidence: 99%

ERF subfamily transcription factors and their function in plant responses to abiotic stresses

Zhang

et al. 2022

Front. Plant Sci.

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“…PCR products were detected by 1% agarose gel electrophoresis (GenStar Kit, GenStar Development Company, Calgary, AB, Canada), and the purified PCR products were inserted into the polyclonal site of the zero-background clone pLB vector (Tiangen Lethal Based Fast Cloning Kit, Beijing, China). The fusion vector GmABR1-pLB was transformed into Escherichia coli DH5α ( Li et al., 2022 ). The positive clones of GmABR1 gene were identified by PCR and enzyme digestion, and the CDS sequence of GmABR1 gene was obtained by sequencing [Sangon Biotech (Shanghai) Co., Ltd., China] ( Wen et al., 2021 ).…”

Section: Methodsmentioning

confidence: 99%

“…They can specifically bind cis -elements in the promoter region of downstream defense genes including activating or inhibiting the expression of downstream stress-responsive genes through positive and negative regulation, and thus participate in the response to plant stress ( Kumar and Sharma, 2021 ). The AP2/ERF transcription factor contains one or two conserved AP2/ERF domains consisting of approximately 58-70 amino acids which are involved in DNA binding ( Li et al., 2022 ). The AP2/ERF domain consists of three β folds and one amphiphilic α helix.…”

Section: Introductionmentioning

confidence: 99%

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GmABR1 encoding an ERF transcription factor enhances the tolerance to aluminum stress in Arabidopsis thaliana

Wang

et al. 2023

Front. Plant Sci.

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The ethylene response factor (ERF) transcription factors, which is one of the largest transcription factor families in plants, are involved in biological and abiotic stress response and play an important role in plant growth and development. In this study, the GmABR1 gene from the soybean inbred line Zhonghuang24 (ZH24)×Huaxia 3 (HX3) was investigated its aluminum (Al) tolerance. GmABR1 protein has a conserved domain AP2, which is located in the nucleus and has transcriptional activation ability. The results of real-time quantitative PCR (qRT-PCR) showed that the GmABR1 gene presented a constitutive expression pattern rich in the root tip, stem and leaf tissues of HX3. After Al stress, the GmABR1 transcript was significantly increased in the roots. The transcripts of GmABR1 in the roots of HX3 treated with 50 µM AlCl3 was 51 times than that of the control. The GmABR1 was spatiotemporally specific with the highest expression levels when Al concentration was 50 µM, which was about 36 times than that of the control. The results of hematoxylin staining showed that the root tips of GmABR1-overexpression lines were stained the lightest, followed by the control, and the root tips of GmABR1 RNAi lines were stained the darkest. The concentrations of Al3+ in root tips were 207.40 µg/g, 147.74 µg/g and 330.65 µg/g in wild type (WT), overexpressed lines and RNAi lines, respectively. When AlCl3 (pH4.5) concentration was 100 µM, all the roots of Arabidopsis were significantly inhibited. The taproot elongation of WT, GmABR1 transgenic lines was 69.6%, 85.6%, respectively. When treated with Al, the content of malondialdehyde (MDA) in leaves of WT increased to 3.03 µg/g, while that of transgenic Arabidopsis increased from 1.66-2.21 µg/g, which was lower than that of WT. Under the Al stress, the Al stress responsive genes such as AtALMT1 and AtMATE, and the genes related to ABA pathway such as AtABI1, AtRD22 and AtRD29A were up-regulated. The results indicated that GmABR1 may jointly regulate plant resistance to Al stress through genes related to Al stress response and ABA response pathways.

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“…Other significant DEGs encoding TFs that have been implicated in aluminum or other abiotic stress responses in previous studies include those encoding ERF in Arabidopsis thaliana (Li et al, 2022), NAC in Solanum lycopersicum (Jin et al, 2020), and MYB in Zea mays (Chen et al, 2018). Overall, most of the TFs obtained in this study were also reported in tea plant, rice and Arabidopsis in response to Al-toxicity (Li et al, 2017;Li et al, 2018) and in finger millet in response to drought and for nutraceutical characteristics (Hittalmani et al, 2017).…”

Section: Differentially Expressed Genesmentioning

confidence: 99%

Finger millet RNA-seq reveals differential gene expression associated with tolerance to aluminum toxicity and provides novel genomic resources

et al. 2022

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Eleusine coracana, finger millet, is a multipurpose crop cultivated in arid and semi-arid regions of Africa and Asia. RNA sequencing (RNA-seq) was used in this study to obtain valuable genomic resources and identify genes differentially expressed between Al-tolerant and Al-susceptible genotypes. Two groups of finger millet genotypes were used: Al-tolerant (215836, 215845, and 229722) and Al-susceptible (212462, 215804 and 238323). The analysis of the RNA-seq data resulted in 198,546 unigenes, 56.5% of which were annotated with significant hits in one or more of the following six databases: NR (48.8%), GO (29.7%), KEGG (45%), PlantTFDB (19.0%), Uniprot (49.2%), and NT (46.2%). It is noteworthy that only 220 unigenes in the NR database had significant hits against finger millet sequences suggesting that finger millet’s genomic resources are scarce. The gene expression analysis revealed that 322 genes were significantly differentially expressed between the Al-tolerant and Al-susceptible genotypes, of which 40.7% were upregulated while 59.3% were downregulated in Al-tolerant genotypes. Among the significant DEGs, 54.7% were annotated in the GO database with the top hits being ATP binding (GO:0005524) and DNA binding (GO:0003677) in the molecular function, DNA integration (GO:0015074) and cell redox homeostasis in the biological process, as well as cellular anatomical entity and intracellular component in the cellular component GO classes. Several of the annotated DEGs were significantly enriched for their corresponding GO terms. The KEGG pathway analysis resulted in 60 DEGs that were annotated with different pathway classes, of which carbohydrate metabolism and signal transduction were the most prominent. The homologs of a number of significant DEGs have been previously reported as being associated with Al or other abiotic stress responses in various crops, including carboxypeptidase SOL1, HMA3, AP2, bZIP, C3H, and WRKY TF genes. A more detailed investigation of these and other DEGs will enable genomic-led breeding for Al tolerance in finger millet. RNA-seq data analysis also yielded 119,073 SNP markers, the majority of which had PIC values above 0.3, indicating that they are highly informative. Additionally, 3,553 single-copy SSR markers were identified, of which trinucleotide SSRs were the most prevalent. These genomic resources contribute substantially to the enrichment of genomic databases for finger millet, and facilitate future research on this crop.

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GsERF1 enhances Arabidopsis thaliana aluminum tolerance through an ethylene-mediated pathway

Cited by 12 publications

References 62 publications

ERF subfamily transcription factors and their function in plant responses to abiotic stresses

ERF subfamily transcription factors and their function in plant responses to abiotic stresses

GmABR1 encoding an ERF transcription factor enhances the tolerance to aluminum stress in Arabidopsis thaliana

Finger millet RNA-seq reveals differential gene expression associated with tolerance to aluminum toxicity and provides novel genomic resources

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