Gu Sui Bu (Drynaria fortunei J. Sm.) antagonizes glucocorticoid-induced mineralization reduction in zebrafish larvae by modulating the activity of osteoblasts and osteoclasts

Peng, Cheng-Huan; Lin, Wen-Ying; Dharini, Kameshwara Kumar; Chang, Chih-Yu; Hong, Jo-Ting; Lin, Ming-Der

doi:10.1016/j.jep.2022.115565

Cited by 8 publications

(5 citation statements)

References 45 publications

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“…Adapting a method from an earlier study ( Peng et al, 2022 ), zebrafish larvae at 7 dpf were stained with 0.2% calcein (C0875, Sigma-Aldrich, USA) and rinsed with egg water. Imaging was performed using a fluorescence stereomicroscope (SMZ25, Nikon, Japan) and ImageJ was used for analysis.…”

Section: Methodsmentioning

confidence: 99%

Potential therapeutic role of spermine via Rac1 in osteoporosis: Insights from zebrafish and mice

Jiang,

Hu,

Deng

et al. 2024

Zoological Research

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Osteoporosis (OP) is a prevalent metabolic bone disease. While drug therapy is essential to prevent bone loss in osteoporotic patients, current treatments are limited by side effects and high costs, necessitating the development of more effective and safer targeted therapies. Utilizing a zebrafish ( Danio rerio ) larval model of osteoporosis, we explored the influence of the metabolite spermine on bone homeostasis. Results showed that spermine exhibited dual activity in osteoporotic zebrafish larvae by increasing bone formation and decreasing bone resorption. Spermine not only demonstrated excellent biosafety but also mitigated prednisolone-induced embryonic neurotoxicity and cardiotoxicity. Notably, spermine showcased protective attributes in the nervous systems of both zebrafish embryos and larvae. At the molecular level, Rac1 was identified as playing a pivotal role in mediating the anti-osteoporotic effects of spermine, with P53 potentially acting downstream of Rac1. These findings were confirmed using mouse ( Mus musculus ) models, in which spermine not only ameliorated osteoporosis but also promoted bone formation and mineralization under healthy conditions, suggesting strong potential as a bone-strengthening agent. This study underscores the beneficial role of spermine in osteoporotic bone homeostasis and skeletal system development, highlighting pivotal molecular mediators. Given their efficacy and safety, human endogenous metabolites like spermine are promising candidates for new anti-osteoporotic drug development and daily bone-fortifying agents.

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Section: Methodsmentioning

confidence: 99%

Potential therapeutic role of spermine via Rac1 in osteoporosis: Insights from zebrafish and mice

Jiang,

Hu,

Deng

et al. 2024

Zoological Research

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“…Based on literature (Boldizsar et al., 2010; Peng et al, 2022), eight genes related to the regulation of osteoblasts were selected, and the primers used in the experiment were synthesized by Sangon Biotech Co., Ltd. The primer sequences are shown in Table 1.…”

Section: Methodsmentioning

confidence: 99%

Anti‐osteoporosis effects of mammalian lignans and their precursors from flaxseed and safflower seed using zebrafish model

Yang,

Wu,

Zhuang

et al. 2023

Journal of Food Science

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Secoisolariciresinol diglucoside (SDG) and tracheloside (TCL) are the main lignan components of flaxseed cake and safflower seed cake, which are by‐products of oil extraction. Both SDG and TCL are metabolized into mammalian lignan enterolactone (EL) with the involvement of intestinal bacteria. In this research, we evaluated the anti‐osteoporosis effects of SDG and the in vivo metabolites EL and enterodiol (ED) prepared in our previous work, as well as the newly isolated chemical constituents from safflower seed, including TCL, the lactone ring opening product of TCL (OTCL) and two alkaloids on the alloxan‐induced zebrafish model. All the compounds showed significant anti‐osteoporosis effects at 80 µM, with p < 0.05 for EL and p < 0.001 for other compounds compared with the model. SDG and TCL showed the most significant and concentration‐dependent effects, with p < 0.001 compared with model at 20 µM. The alkaloids, N‐coumaroylserotonin glucoside and N‐feruloylserotonin glucoside, also showed anti‐osteoporosis at 20 µM with p < 0.01, whereas EL, ED, and OTCL showed no significant effects. Quantitative real‐time polymerase chain reaction revealed that SDG and TCL upregulated the expression of osteogenic genes Runx2, SP7, OPG, Col1a1a, Alp, ON, OPN, and OCN in alloxan‐treated zebrafish. The in vivo metabolite of lignans, EL, showed significant anti‐inflammatory effect (p < 0.01) at 20 µM, which might also help to combat osteoporosis and other complications caused by excessive immune response in the body. The results provided scientific data for using the oil extraction by‐products as sources of anti‐osteoporosis compounds.Practical ApplicationThis study found that lignans in flaxseed cake and safflower seed cake exhibited anti‐osteoporosis effects by upregulating the expression of osteogenic genes, making the oil extraction by‐products sources of anti‐osteoporosis compounds.

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“…Drynariae Rhizoma is introduced to improve glucocorticoid-induced OP by regulating the activity of osteoblasts and osteoclasts. Naringin has been identified as an effective anti-OP component of Drynariae Rhizoma ( Peng C.-H. et al, 2022 ). Like naringin, the other five flavonoids, namely, aglycones, kurarinone, kushennol F, xanthogalenol, and sophoraflavanone G, have a potential protective effect against ovariectomized-induced osteoporosis, which may be related to the activation of endoplasmic reticulum signaling pathways ( Wang et al, 2011 ).…”

Section: Tcm In the Treatment Of Op Based On Syndrome Differentiationmentioning

confidence: 99%

Traditional Chinese medicine in osteoporosis: from pathogenesis to potential activity

Cao,

Hu,

Ning

et al. 2024

Front. Pharmacol.

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Osteoporosis characterized by decreased bone density and mass, is a systemic bone disease with the destruction of microstructure and increase in fragility. Osteoporosis is attributed to multiple causes, including aging, inflammation, diabetes mellitus, and other factors induced by the adverse effects of medications. Without treatment, osteoporosis will further progress and bring great trouble to human life. Due to the various causes, the treatment of osteoporosis is mainly aimed at improving bone metabolism, inhibiting bone resorption, and promoting bone formation. Although the currently approved drugs can reduce the risk of fragility fractures in individuals, a single drug has limitations in terms of safety and effectiveness. By contrast, traditional Chinese medicine (TCM), a characteristic discipline in China, including syndrome differentiation, Chinese medicine prescription, and active ingredients, shows unique advantages in the treatment of osteoporosis and has received attention all over the world. Therefore, this review summarized the pathogenic factors, pathogenesis, therapy limitations, and advantages of TCM, aiming at providing new ideas for the prevention and treatment of OP.

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Gu Sui Bu (Drynaria fortunei J. Sm.) antagonizes glucocorticoid-induced mineralization reduction in zebrafish larvae by modulating the activity of osteoblasts and osteoclasts

Cited by 8 publications

References 45 publications

Potential therapeutic role of spermine via Rac1 in osteoporosis: Insights from zebrafish and mice

Potential therapeutic role of spermine via Rac1 in osteoporosis: Insights from zebrafish and mice

Anti‐osteoporosis effects of mammalian lignans and their precursors from flaxseed and safflower seed using zebrafish model

Traditional Chinese medicine in osteoporosis: from pathogenesis to potential activity

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