Guggulsterone inhibits melanogenesis in B16 murine melanoma cells by downregulating tyrosinase expression

Koo, Jeung-Hyun; Rhee, Kyoung‐Suk; Koh, Hyoung-Won; Jang, Hyun-Young; Park, Byung‐Hyun; Park, Jin-Woo

doi:10.3892/ijmm.2012.1057

Cited by 15 publications

(8 citation statements)

References 27 publications

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“…The synthesis of melanin in melanocytes can be induced by numerous factors, including α-MSH, cyclic adenosine monophosphate (cAMP)-elevating agents (such as isobutylmethylxanthine and forskolin) and UV light [23,24]. Finding agents to regulate melanin synthesis is important for the treatment of hyper- or hypo-pigmentation [25,26,27].…”

Section: Resultsmentioning

confidence: 99%

Effects of Danshensu and Salvianolic Acid B from Salvia miltiorrhiza Bunge (Lamiaceae) on Cell Proliferation and Collagen and Melanin Production

et al. 2014

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Danshensu (DSU) and salvianolic acid B (SAB) are the primary water-soluble compounds of Salvia miltiorrhiza Bunge (Lamiaceae). In this study, we analyzed the effects of DSU, SAB and a S. miltiorrhiza extract (SME) on cell proliferation. Additionally, the effects of DSU and SAB on collagen synthesis in Detroit 551 human normal fibroblast cells and on melanin production in B16 melanoma cells were verified. The results demonstrated that SME can enhance the proliferation of Detroit 551 cells and that this boost may be caused by DSU and SAB. This research showed that SME, DSU and SAB all have the ability to increase the production of collagen in Detroit 551 cells. The results also confirmed that DSU and SAB can attenuate the α-MSH-stimulated melanin production of B16 cells by inhibiting tyrosinase activity. Therefore, SME, DSU and SAB each have the potential to be utilized as active ingredients in wound healing or cosmetic treatments. In the future, DSU and SAB could also be used as functional components for treating hyperpigmentation.

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Section: Resultsmentioning

confidence: 99%

Effects of Danshensu and Salvianolic Acid B from Salvia miltiorrhiza Bunge (Lamiaceae) on Cell Proliferation and Collagen and Melanin Production

et al. 2014

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“…Second, we found that melanogenesis of B16F10 cells by the MA extract (20 µg/ml) was rapid and that the pellet color of cells was enhanced within 4 h after treatment. To compare the melanogenic effect of MA with other agents, we used the well-known melanogenic inducer forskolin at a concentration (20 µM) that is higher than the concentrations (5 or 10 µM) usually used in other groups (34,35). After incubation with the MA extract (20 µg/ml) for 8 h, the pellet color was darker than that incubated with forskolin (20 µM) for 48 h (Fig.…”

Section: Discussionmentioning

confidence: 99%

Melia azedarach extract stimulates melanogenesis through increase of tyrosinase-related protein 1 expression in B16F10 mouse melanoma cells

Cheng

Jin

et al. 2015

International Journal of Molecular Medicine

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Abstract. Melia azedarach (MA) has been used in folk medicine in Asia for the treatment of several diseases. Several constituents from MA possess anti-herpetic, anti-angiogenic and anticancer properties. The aim of the present study was to investigate the effect of a 70% ethanol extract of MA on melanogenesis and the underlying mechanisms involved. A B16F10 mouse melanoma cell line was used in our experiments. Treatment of B16F10 cells with the MA extract (10, 20 and 40 µg/ml) increased melanin content in a concentration-dependent manner without cytotoxicity at 24 h. Further experiments indicated that the MA extract (20 µg/ml) increased melanin content as early as at 4 h after treatment. Additionally, although the MA extract did not affect intracellular tyrosinase activity and the protein levels of tyrosinase and tyrosinase-related protein-2 (TRP-2) at 2 and 4 h after treatment, the MA extract increased TRP-1 protein expression at both time points. However, no significant effect of the MA extract treatment on TRP-1 mRNA level at the time points measured was observed. In conclusion, the results from the present study demonstrate that the MA extract increases melanogenesis through the upregulation of TRP-1 protein expression by post-transcriptional control in B16F10 cells and suggest that the MA extract can be viewed as a rapid inducer of melanogenesis, thus rendering it a potential treatment for hypopigmentation diseases including vitiligo.

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“…Microphthalmia-associated transcription factor (MITF), which is one of the most important nuclear activation transcription factors of tyrosinase, TRP-1 and TRP-2, plays key role in melanocyte development regulation and survival [37, 38]. Our results showed that apigenin-7-butylene glucoside had no effects on the MITF transcription and translation levels in B16-F10 cells as shown in Figures 5 and 6, though the MITF was reported to be the master regulator in the transcription of genes which were involved in melanin synthesis.…”

Section: Discussionmentioning

confidence: 99%

A Newly Authenticated Compound from Traditional Chinese Medicine Decoction Induces Melanogenesis in B16‐F10 Cells by Increasing Tyrosinase Activity

Xin

Zou

et al. 2018

Evidence-Based Complementary and Alternative Medicine

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Vitiligo is a kind of skin dysfunction on melanogenesis. The highly prevalent, chronic, and distinctive complexion changes on patients have imposed enormous psychic and economic burden on both individuals and society. Traditional Chinese Medicine (TCM) is a kind of precious source on chronic disease treatment, including skin dysfunctional diseases. In our previous study, a new compound named apigenin-7-butylene glucoside has been authenticated and purified from a prescription of Chinese traditional medicine formula which has been used clinically in vitiligo treatment. The aim of this work is to evaluate the effects of this compound on melanogenesis using melanoma cell B16-F10 in vitro. The results showed that apigenin-7-butylene glucoside had almost no cytotoxicity on B16-F10 cells within a lower dose of 5.0 μg ml−1 and enhanced the melanin level to about 41% and tyrosinase activity to 1.32-fold when compared with controls. The compound showed minor cytotoxicity to B16-F10 cells at the higher concentration of 10 μg ml−1 and 50 μg ml−1, the inhibition rate was 8.4% and 11.8%, and the melanin level and tyrosinase activity showed a decreased trend because of the lower cell number at the higher concentrations. The results indicated that apigenin-7-butylene glucoside was safe to B16-F10 cells within a lower concentration, <5.0 μg ml−1. Incubated with 5.0 ug ml−1of apigenin-7-butylene glucoside for 48 hours, the mRNA and protein levels of Tyr, Trp-1, and Trp-2 genes were all increased except Mitf in B16-F10 cells. The stimulation of apigenin-7-butylene glucoside on melanogenesis of B16-F10 cells through Tyr, Trp-1, and Trp-2 pathway highlighted the potential usage of the compound in vitiligo treatment.

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Guggulsterone inhibits melanogenesis in B16 murine melanoma cells by downregulating tyrosinase expression

Cited by 15 publications

References 27 publications

Effects of Danshensu and Salvianolic Acid B from Salvia miltiorrhiza Bunge (Lamiaceae) on Cell Proliferation and Collagen and Melanin Production

Effects of Danshensu and Salvianolic Acid B from Salvia miltiorrhiza Bunge (Lamiaceae) on Cell Proliferation and Collagen and Melanin Production

Melia azedarach extract stimulates melanogenesis through increase of tyrosinase-related protein 1 expression in B16F10 mouse melanoma cells

A Newly Authenticated Compound from Traditional Chinese Medicine Decoction Induces Melanogenesis in B16‐F10 Cells by Increasing Tyrosinase Activity

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