Guidance of stem cell fate on 2D patterned surfaces

Kolind, Kristian; Leong, Kam W.; Besenbacher, Flemming; Foss, Morten

doi:10.1016/j.biomaterials.2012.05.070

Cited by 150 publications

(124 citation statements)

References 69 publications

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“…Tremendous advancements have been made in tailoring bone biomaterials to modulate stem cell fates, based on two general strategies: (1) modulation of the physical and chemical properties of biomaterial surfaces,2 and (2) incorporation of bioactive cues onto biomaterial surfaces 3. For instance, electroactive biomaterials have been designed to stimulate the osteogenesis of stem cell, thereby inducing subsequent bone regeneration.…”

Section: Introductionmentioning

confidence: 99%

Valence State Manipulation of Cerium Oxide Nanoparticles on a Titanium Surface for Modulating Cell Fate and Bone Formation

Wen

et al. 2017

Advanced Science

129

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Understanding cell–biomaterial interactions is critical for the control of cell fate for tissue engineering and regenerative medicine. Here, cerium oxide nanoparticles (CeONPs) are applied at different Ce4+/Ce3+ ratios (i.e., 0.46, 1.23, and 3.23) to titanium substrate surfaces by magnetron sputtering and vacuum annealing. Evaluation of the cytotoxicity of the modified surface to cultured rat bone marrow mesenchymal stem cells (BMSCs) reveals that the cytocompatibility and cell proliferation are proportional to the increases in Ce4+/Ce3+ ratio on titanium surface. The bone formation capability induced by these surface modified titanium alloys is evaluated by implanting various CeONP samples into the intramedullary cavity of rat femur for 8 weeks. New bone formation adjacent to the implant shows a close relationship to the surface Ce4+/Ce3+ ratio; higher Ce4+/Ce3+ ratio achieves better osseointegration. The mechanism of this in vivo outcome is explored by culturing rat BMSCs and RAW264.7 murine macrophages on CeONP samples for different durations. The improvement in osteogenic differentiation capability of BMSCs is directly proportional to the increased Ce4+/Ce3+ ratio on the titanium surface. Increases in the Ce4+/Ce3+ ratio also elevate the polarization of the M2 phenotype of RAW264.7 murine macrophages, particularly with respect to the healing‐associated M2 percentage and anti‐inflammatory cytokine secretion. The manipulation of valence states of CeONPs appears to provide an effective modulation of the osteogenic capability of stem cells and the M2 polarization of macrophages, resulting in favorable outcomes of new bone formation and osseointegration.

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Section: Introductionmentioning

confidence: 99%

Valence State Manipulation of Cerium Oxide Nanoparticles on a Titanium Surface for Modulating Cell Fate and Bone Formation

Wen

et al. 2017

Advanced Science

129

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“…24 Ideally, the scaffold should mimic the cells' microenvironment, giving the required structural signals, adhesion molecules, and pore size for homing, differentiation, and phenotypic acquisition, 25 while allowing cell-cell and cellmatrix interactions. 26 Even minute differences in the scaffold geometry, pore size, elasticity, mechanical properties, chemical composition, and degradation rate can greatly influence the cells regenerative behavior in vivo.…”

Section: Discussionmentioning

confidence: 99%

Stem Cell Transplantation for Pulpal Regeneration: A Systematic Review

El‐Sayed

Jakusz

Jochens³

et al. 2015

Tissue Engineering Part B: Reviews

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For treating pulpal pathological conditions, pulpal regeneration through transplanted stem/progenitor cells might be an alternative to conventional root canal treatment. A number of animal studies demonstrated beneficial effects of stem/progenitor cell transplantation for pulp-dentin complex regeneration, that is, pulpal tissue, neural, vascular, and dentinal regeneration. We systematically reviewed animal studies investigating stem/progenitor cell-mediated pulp-dentin complex regeneration. Studies quantitatively comparing pulp-dentin complex regeneration after transplantation of stem/progenitor cells versus no stem/progenitor cell transplantation controls in intraoral in vivo teeth animal models were analyzed. The following outcomes were investigated: regenerated pulp area per root canal total area, capillaries per total surface, regenerated dentinal area per total defect area, and nerves per total surface. PubMed and EMBASE were screened for studies published until July 2014. Crossreferencing and hand searching were used to identify further articles. Standardized mean differences (SMD) and 95% confidence intervals (95% CI) were calculated using random-effects meta-analysis. To assess possible bias, SYRCLE's risk of bias tool for animal studies was used. From 1364 screened articles, five studies (representing 64 animals) were included in the quantitative analysis. Risk of bias of all studies was high. Stem/progenitor celltransplanted pulps showed significantly larger regenerated pulp area per root canal total area (SMD [95% CI]: 2.28 [0.35-4.21]) and regenerated dentin area per root canal total area ]) compared with no stem/progenitor cell transplantation controls. Only one study reported on capillaries per or nerves per total surface and found both significantly increased in stem/progenitor cell-transplanted pulps compared with controls. Stem/progenitor cell transplantation seems to enhance pulp-dentin complex regeneration in animal models. Due to limited data quantity and quality, current evidence levels are insufficient for further conclusions.

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“…[2,4] Microfluidic and nanotopographic patterning approaches establish some organ-level functions ex vivo, but lack the dimensionality to approximate developing tissues. [2,[12][13][14] Light is an ideal stimulus for perturbing the spatiotemporal dynamics of signals in living cells and organisms with high resolution. [15][16][17] The light-based optogenetic field has answered real biological questions [16] and developed tools for light-controlled genome editing and gene transfection.…”

Section: Doi: 101002/adma201603318mentioning

confidence: 99%