Guideline for the Care and Use of Laboratory Animals in Iran

Ahmadi‐Noorbakhsh, Siavash; Ardakani, Esmat Mirabzadeh; Sadighi, Jila; Aldavood, Seyed Javid; Abbasi, Mohammad Farajli; Farzad‐Mohajeri, Saeed; Ghasemi, Asghar; Sharif‐Paghaleh, Ehsan; Hatami, Zahra; Nikravanfard, Nazila; Gooshki, Ehsan Shamsi

doi:10.1038/s41684-021-00871-3

Cited by 54 publications

(32 citation statements)

References 3 publications

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“…All rats had free access to food and tap water. The entire experimental procedures were in accordance with a guideline provided by the National Institute of Health for the Care and Use of Laboratory Animals (Ahmadi‐Noorbakhsh et al, 2021) with an ethics code of IR.TBZMED.VCR. REC.1397.276.…”

Section: Methodsmentioning

confidence: 99%

The effect of Fumaria parviflora on the expression of sexual hormones along with their receptors in testicles of adult rats induced by varicocele

et al. 2022

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Varicocele (VCL) is a pathological dilation of the venous pampiniform plexus of the spermatic cord and is also classified as male factor infertility. The current experiment aimed to examine the protective effect of Fumaria parviflora (FP), as a powerful antioxidant, against reproductive damage induced by VCL. In this experimental study, 32 male rats were randomly allocated into four groups, namely sham (simple laparotomy without additional intervention), FP (healthy rats administered 250 mg/kg FP), VCL + FP (underwent VCL and received 250 mg/kg FP), VCL (underwent VCL without receiving any treatment). The results showed that the number of Sertoli and germ cells were markedly reduced in the VCL group in comparison to the FP‐treated and sham groups. The VCl + FP group had significantly higher serum levels of testosterone (T), FSH, and LH hormones than the VCL group. The quality and motility of spermatozoa were reduced in the VCL group compared with other groups (p ≤ 0.05). Moreover, our findings demonstrated that the administration of FP considerably enhanced the mRNA levels of CatSper‐1 and ‐2, SF‐1, 3β‐HSD, 17β‐HSD3, LHCGR, and FSHR (p ≤ 0.05). Based on the obtained results, treatment with FP is capable of preventing testicular dysfunction and elevating the concentration of hormones and some crucial genes, such as CatSper1 and 2, SF‐1, 3β‐HSD, 17β‐HSD3, LHCGR, and FSHR that contribute to the spermatogenesis process.

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Section: Methodsmentioning

confidence: 99%

The effect of Fumaria parviflora on the expression of sexual hormones along with their receptors in testicles of adult rats induced by varicocele

et al. 2022

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“…In the proposed workshop plan, no animal is used for ‘educational’ purposes and their use are only limited to training purposes. We recommend using surplus stock animals and animals from completed studies, that had been fully recovered from prior interventions with a severity score of mild to moderate [ 7 ]. The severity score of the proposed workshop plan is ‘mild’ and we use anesthetic/analgesic drugs for all animals undergoing painful interventions.…”

Section: Discussionmentioning

confidence: 99%

“…For developing the workshop plan, we used the experience gained through holding more than 60 laboratory animal workshops in the country. We also adhered to the principles outlined in our Guideline for the Care and Use of Laboratory Animals [ 7 ]. We first defined the scientific and ethical objectives of the workshop.…”

Section: Main Textmentioning

confidence: 99%

A proposed framework for holding intensive 3Rs workshops in laboratory animal science

et al. 2022

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Principles of 3Rs are the backbone of today’s animal research. Applying 3Rs in practice requires proper education and training. Although this could be achieved via long-term courses ranging from several weeks to years, in some circumstances a short-term course may be the only viable option. In this paper we define scientific and ethical objectives for running short-term 3Rs workshops. To meet these objectives, we propose a 2-day workshop curriculum and an ethical framework. The curriculum comprises theoretical and practical sessions and covers Replacement, Reduction, and Refinement strategies. The ethical framework defines the responsibilities of lecturers and attendees, and proposes the animals and facilities requirements to run the proposed workshop curriculum. The attendees will be assessed at the end of the workshop and they receive certificates for working under supervision until they are deemed competent at their intended functions. The proposed curriculum and framework are not subscriptive, instead they share the experience gained through running more than 60 laboratory animal 3Rs workshops.

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“…This study was approved by the Ethics Committee of Tehran University of Medical Sciences (Approval code: IR.TUMS.MEDICINE.REC.1399.944) and was performed in compliance with the guideline for the care and use of laboratory animals in Iran [ 38 ].…”

Section: Methodsmentioning

confidence: 99%

Photoactivation of Curcumin Doped Poly-Lactic-Co-Glycolic Acid Nanoparticles in Rat Model with Fixed Orthodontic Appliances

Ghorbanpour

Pourhajibagher

Noroozian

et al. 2022

The Scientific World Journal

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This study aimed to evaluate the antimicrobial effect of curcumin doped poly-lactic-co-glycolic acid nanoparticles (Cur-PLGA-Nps)-mediated antimicrobial photodynamic therapy (aPDT), as well as the probiotics on S. mutans in rats with fixed orthodontic appliances. Orthodontic appliances were ligated to the right maxillary of the rats. After the oral colonization of S. mutans, the rats were then treated in four groups including Cur-PLGA-Nps, light-emitting diode, Cur-PLGA-Nps-mediated aPDT, and probiotic (Lactobacillus acidophilus). After that, the S. mutans counts and the gtfB gene expression of S. mutans were determined on days 4, 7, 15, and 30. Probiotic and Cur-PLGA-Nps-mediated aPDT groups significantly reduced the count of S. mutans in a time-dependent manner ( P < 0.05 ). So, probiotics and Cur-PLGA-Nps-mediated aPDT were able to reduce S. mutans more than other groups on the 30th day. Also, there was no considerable difference between Cur-PLGA-Nps-mediated aPDT and probiotic groups in bacterial growth inhibition. The expression level of gtfB gene was significantly downregulated at all-time intervals after exposure to Cur-PLGA-Nps-mediated aPDT compared with untreated bacteria ( P < 0.05 ). According to the results, simultaneous use of Cur-PLGA-Nps-mediated aPDT and probiotic therapeutic approaches can be suggested to increase effectiveness.

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Guideline for the Care and Use of Laboratory Animals in Iran

Cited by 54 publications

References 3 publications

The effect of Fumaria parviflora on the expression of sexual hormones along with their receptors in testicles of adult rats induced by varicocele

The effect of Fumaria parviflora on the expression of sexual hormones along with their receptors in testicles of adult rats induced by varicocele

A proposed framework for holding intensive 3Rs workshops in laboratory animal science

Photoactivation of Curcumin Doped Poly-Lactic-Co-Glycolic Acid Nanoparticles in Rat Model with Fixed Orthodontic Appliances

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