GUS Gene expression and plant regeneration via somatic embryogenesis in cucumber (Cucumis sativus L.)

Kim, Hyun-A; Lee, Boo-Youn; Jeon, Jin-Jung; Choi, Dong-Woog; Choi, Pil Son; Utomo, Setyo Dwi; Lee, Jae-Hyoek; Kang, Tong-Ho; Lee, Young Jin

doi:10.5010/jpb.2008.35.4.275

Cited by 6 publications

(5 citation statements)

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“…The cucumber plants were generally known as a recalcitrant plant species because of non-repeatability and genotype dependence (Gaba et al 2004). To improve the efficiency of cucumber transformation, new stable transformation system have been developed by using of an alternative selectable marker (Cho et al 2005a) or a regeneration system via somatic embryogenesis (Kim et al 2008). However, a few problems in (Cho et al 2005a;Kim et al 2008).…”

Section: Resultsmentioning

confidence: 99%

“…Transgenic cucumber resistant to virus has been developed using Agrobacteriummediated transformation (Gaba et al 2004). Up to now, the transformation protocols for cucumber have been optimized in various aspects involving in transformation methods, explants types, Agrobacterium strains, Agrobacterium concentration, length of exposure time to Agrobacterium, and selection markers, etc (Chee 1990;Kodama et al 1993;Kose and Koç 2003;Lin et al 2011;Kim et al 2008). Agrobacterium-mediated genetic transformation is proved to be a reliable way for transferring foreign genes into cucumber genome, and a stable transformation system has been improved without chimerical transgenic event (Jang et al 2011).…”

Section: Introductionmentioning

confidence: 99%

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Production of transgenic cucumber expressing phytoene synthase-2A carotene desaturase gene

et al. 2016

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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.Abstract The objectives of this study were to 1) evaluate the efficiency of the protocol of Agrobacterium-mediated transformation of cucumber to introduce phytoene synthase-2a carotene desaturase (PAC genes); 2) demonstrate the integration of PAC genes into the genome of putative transgenic cucumber based on growth on selection medium, PCR and Southern analysis; 3) evaluate the expression of PAC genes in transgenic cucumber based on the analysis of RT-PCR and Northern blot hybridization. Out of 5,945 cotyledonary-node explants inoculated with Agrobacterium, 65 (1.1%) explants produced 238 shoots. Integration of PAC genes into the genome of the cucumber was demonstrated based on the analysis of gDNA-PCR, 21 out of the 238 plants regenerated; while 6 plants proved positive for Southern blot hybridization. Transgene expression was demonstrated based on analysis of RT-PCR, 6 plants proved positive out of the 6 plants analyzed; while 4 plants out of 6 proved positive during Northern blot hy-bridization. This study successfully demonstrated the production of transgenic cucumber, integration, and expression of the PAC gene in cucumber.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Production of transgenic cucumber expressing phytoene synthase-2A carotene desaturase gene

et al. 2016

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“…However, a few problems in these transformation system have caused difficulty such as low frequency (%) of gene transfer to target cells, transgenic cucumber with chimeric, and not easily regenerated from somatic embryogenesis (Cho et al . 2005a;Kim et al 2008). In our study, it was still showed at a high frequency (3.5%) of chimeric plants.…”

Section: Results and Dicussionmentioning

confidence: 99%

“…In general, the cucumber was known as a recalcitrant plant species because of non-repeatable, researcher and genotype dependence (Gaba et al 1996). To solve the problem, a new stable transformation system have been developed by using of selection marker gene such as phospinotricin agent (Cho et al 2005a) or by using of regeneration system via somatic embryogenesis (Kim et al 2008). However, a few problems in these transformation system have caused difficulty such as low frequency (%) of gene transfer to target cells, transgenic cucumber with chimeric, and not easily regenerated from somatic embryogenesis (Cho et al .…”

Section: Results and Dicussionmentioning

confidence: 99%

“…Using Agrobacteriummediated transformation technology, cultivars with superior agronomic characters such as virus resistance (Gaba et al 2004) could be developed. Although the cucumber was known as a recalcitrant plant species, a stable transformation system has been improved without chimerical transgenic event (Kim et al 2008). Abiotic factors such as low temperature, drought, and high salinity are common stress conditions that adversely affect the plant growth and yield.…”

Section: Introductionmentioning

confidence: 99%

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Development of transgenic cucumber expressing TPSP gene and morphological alterations

Kim¹,

Min²,

Choi³

et al. 2010

Journal of Plant Biotechnology

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To develop transgenic cucumber tolerant to abiotic stress, a cotyledonary-node explants were co-cultivated with Agrobacterium tumefaciens (EHA101) carrying TPSP gene (pHC30-TPSP). After transfer to fresh medium every two week for eight weeks, putative transgenic plants were selected when shoots grown a length greater than 3 cm from the cotyledonary-node explants on selection medium supplemented with 5 mgl -1 phospinotricin as selectable agent. The confirmation of transgenic cucumber was based on the Northern blot analysis. Thirty four shoots (5.2%) with resistance to phospinotricin were obtained from 660 explants inoculated. Of them, transformants were only confirmed from 11 plants (1.7%). Transgenic cucumber expressing TPSP gene was more synthesized at 3.8 times amounts of trehalose (0.014 mg g fresh wt ). However, all of transgenic plants showed abnormal morphology, including stunted growth (< height 15 cm), shrunken leaves, and sterility as compared with non-transgenic plants (> height 150 cm) under the same growth environment. These results lead us to speculate that the overproduction of trehalose was toxic for cucumber, even though that had known for rice as non-toxic.

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Current status of genetic transformation technology developed in cucumber (Cucumis sativus L.)

Wang

et al. 2015

Journal of Integrative Agriculture

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Genetic transformation is an important technique for functional genomics study and genetic improvement of plants. Until now, Agrobacterium-mediated transformation methods using cotyledon as explants has been the major approach for cucumber, and its frequency has been up to 23%. For example, significantly enhancement of the transformation efficiency of this plant species was achieved from the cotyledon explants of the cultivar Poinsett 76 infected by Agrobacterium strains EHA105 with efficient positive selection system in lots of experiments. This review is to summarize some key factors influencing cucumber regeneration and genetic transformation, including target genes, selection systems and the ways of transgene introduction, and then to put forward some strategies for the increasing of cucumber transformation efficiency. In the future, it is high possible for cucumber to be potential bioreactor to produce vaccine and biomaterials for human beings.

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GUS Gene expression and plant regeneration via somatic embryogenesis in cucumber (Cucumis sativus L.)

Cited by 6 publications

References 10 publications

Production of transgenic cucumber expressing phytoene synthase-2A carotene desaturase gene

Production of transgenic cucumber expressing phytoene synthase-2A carotene desaturase gene

Development of transgenic cucumber expressing TPSP gene and morphological alterations

Current status of genetic transformation technology developed in cucumber (Cucumis sativus L.)

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