GUS Gene Transformation in Rice (Oryza sativa L.) Variety BRRI Dhan-30 Mediated by Agrobacterium tumefaciens

Asaduzzaman, Md.; Bari, M. A.; Rahman, Maiada M.A.; Minami, Mineo; Matsushima, K.; Nemoto, Kazuhiro

doi:10.3923/biotech.2008.530.536

Cited by 5 publications

(3 citation statements)

References 23 publications

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“…In previous study it were showed that the callus induction frequency were between 60% and 90% [22], in the present study the callus induction rate reached up to 72% that support the previous study. However, high callus induction and regeneration may differ in different media composition and varieties [4], [5].…”

Section: Discussionsupporting

confidence: 91%

Studies on In Vitro Response to Callus Induction and Regeneration of Five High Yielding Indica rice Varieties

Khan

Islam²,

Islam³

2019

JHPR

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Due to growing population, there is an increasing demand of rice production but the productivity of rice is lessened day by day. To overcome this problem various biotechnological tools can be used for developing various rice varieties. However, the lack of a simple and efficient protocol for callus induction, embryogenic callus formation and quick plant regeneration in this cereal crop. In this study embryogenic calli from mature seeds of five indica rice varieties viz. Binadhan-5, Binadhan-6, BRRI dhan-48, BRRI dhan-58 and IR-64 were observed that is done in four different types of media composition. The highest callus induction were observed in media containing Sucrose as a carbon source. Among those varieties Binadhan-6 and BRRI dhan-48 showed highest rate of callus induction respectively. This study will be useful for selecting suitable callus induction medium for callus induction in future that will be useful for not only national but also international plant breeders for producing new variety and so on.

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Section: Discussionsupporting

confidence: 91%

Studies on In Vitro Response to Callus Induction and Regeneration of Five High Yielding Indica rice Varieties

Khan

Islam²,

Islam³

2019

JHPR

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“…Our results showed that the explant pollution and mortality rates increased after 20-min infection. AS is widely used in cotton (Sunilkumar and Rathore 2001), rice (Asaduzzaman et al 2008), and kenaf (Srivatanakul et al 2001) for enhancing the transfer rate of T-DNA from Agrobacterium to plant cells. Our findings show that AS also had the same effect in jute ( Figure 3D Results are the mean of 3 replication ± SD.…”

Section: Genetic Transformation and Gus Histochemical Assaymentioning

confidence: 99%

An Efficient Regeneration System and Optimization of the Transformation from the Cotyledonary Node of Jute (Corchorus capsularisL.)

Zhang

et al. 2015

Journal of Natural Fibers

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The purpose of this study was to use cotyledonary nodes as explants to establish an efficient regeneration protocol for jute (Corchorus capsularis L.) on Murashige and Skoog (MS) basal medium. Research into the different growth regulators using the orthogonal design L16 (4 5 ) revealed that the best shoot induction medium is MS medium containing 8% (w/v) agar and 3% (w/v) sucrose supplemented with 1.0 mg/L 6BA and 0.25 mg/L NAA. The average number of shoots per explant and the explant induction rate were 9.8 and 100%. After 3 weeks, 2-3 cm shoots were rooting on 1/2 MS medium containing 8% (w/v) agar and 3% (w/v) sucrose supplemented with 1.0 mg/L 6-BA + 0.2 mg/L NAA. Moreover, we optimized Agrobacterium-mediated transformation using the GUS gene transient expression system. The best condition for obtaining higher transformation rate consisted of the use of fresh explants to which 100 µM acetosyringone was added for a co-culture time of 10 min, the OD value of Agrobacterium liquid is 0.5 at 600 nm. These data provide an important basis for the application of other trait gene in the improvement of jute fiber quality.

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“…Acetosyringone (AS) is known to enhance the transfer of T-DNA from Agrobacterium to plant cells in many plants like cotton (Sunilkumar and Rathore 2001), kenaf (Srivatanakul et al 2001), rice (Asaduzzaman et al 2008) etc. The primary step in genetic transformation is the attachment of Agrobacterium to the host plant.…”

Section: Addition Of Acetosyringonementioning

confidence: 99%

In vitro regeneration and optimization of conditions for Agrobacterium mediated transformation in jute, Corchorus capsularis

Pushyami

Beena

Sinha

et al. 2011

J. Plant Biochem. Biotechnol.

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Jute is a crop of commercial importance that is widely cultivated for its bast fiber production but susceptible to many diseases that results in major economic loss. New genes can be introduced into this plant through Agrobacterium mediated genetic transformation for its genetic improvement, which is dependent on the availability of suitable in vitro techniques. An efficient regeneration system has been developed for in vitro culture of jute (Corchorus capsularis) from the distal cut ends of cotyledonary petioles. High frequency shoot regeneration was obtained on Murashige and Skoog (MS) nutrient agar medium supplemented with 0.5 mg/l NAA, 0.5 mg/l BAP and 36 g/l sucrose. On transfer to soil, the regenerated plantlets survived and appeared to be morphologically similar to the normal seed-grown plants. They developed pods and set fertile seeds. Histological analysis revealed de novo origin of shoot buds in the in vitro cultured cotyledonary petioles. Parameters affecting transformation were optimized by assaying GUS activity in these regenerable tissues after cocultivation with Agrobacteria. These tissues appear to be susceptible for infection and transformation by Agrobacterium carrying uid (GUS INT) and nptII genes, as well as shoot multiplication. The cells at the cut end of the petioles were found competent to take up the DNA, which was monitored by transient GUS gene expression. EHA105 at 0.3 O.D and LBA4404 at 0.5 O.D were found to be compatible in giving optimal levels of transient GUS expression.

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GUS Gene Transformation in Rice (Oryza sativa L.) Variety BRRI Dhan-30 Mediated by Agrobacterium tumefaciens

Cited by 5 publications

References 23 publications

Studies on <i>In Vitro </i>Response to Callus Induction and Regeneration of Five High Yielding <i>Indica </i>rice Varieties

Studies on <i>In Vitro </i>Response to Callus Induction and Regeneration of Five High Yielding <i>Indica </i>rice Varieties

An Efficient Regeneration System and Optimization of the Transformation from the Cotyledonary Node of Jute (Corchorus capsularisL.)

In vitro regeneration and optimization of conditions for Agrobacterium mediated transformation in jute, Corchorus capsularis

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