Gut barrier impairment by high‐fat diet in mice depends on housing conditions

Müller, Veronika Maria; Zietek, Tamara; Rohm, Florian; Fiamoncini, Jarlei; Lagkouvardos, Ilias; Haller, Dirk; Clavel, Thomas; Daniel, Hannelore

doi:10.1002/mnfr.201500775

Cited by 57 publications

(44 citation statements)

References 39 publications

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“…As expected, housing conditions markedly influenced the composition of the microbiota in mice (this study and reviewed in [39]) and intestinal barrier integrity in mice fed a HFD [40]. Future studies should consider environmental conditions to be one of the most important factors affecting the composition of the mouse gut microbiome.…”

Section: Discussionsupporting

confidence: 54%

Prolonged transfer of feces from the lean mice modulates gut microbiota in obese mice

et al. 2016

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BackgroundTransplanting a fecal sample from lean, healthy donors to obese recipients has been shown to improve metabolic syndrome symptoms. We therefore examined the gut microbiota in mice after administering a long-term, high-fat diet (HFD) supplemented with feces from lean mice through the fecal-oral route.MethodsC57BL6/W mice were allowed to adapt to a non-specific pathogen free (SFP) environment for 2 weeks before being divided into three groups of 16 animals. Animals were fed for 28 weeks with a normal diet (ND), HFD or HFD supplemented with feces from ND-fed mice (HFDS). The composition of colonizing bacteria was evaluated in droppings collected under SPF conditions at the beginning of the study and at 12 and 28 weeks using an 16S Metagenomics Kit on Ion PGM sequencer.ResultsHFD and HFDS-fed mice attained (p < 0.05) greater body weights by weeks 6 and 5, respectively. HFDS-fed mice gained more weight than HFD-fed mice by week 25. Both species diversity and richness indices increased with time in HFDS mice only.ConclusionsProlonged HFD-fed mice supplementation with feces from lean mice altered bacteria species diversity and richness, accelerated the onset of obesity, and caused increased weight gain in the later weeks of the HFD regimen.Electronic supplementary materialThe online version of this article (doi:10.1186/s12986-016-0116-8) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 54%

Prolonged transfer of feces from the lean mice modulates gut microbiota in obese mice

et al. 2016

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“…The metabolic endotoxaemia hypothesis, which states that high dietary fat intake impairs the gut barrier, is largely based on data from dietary interventions studies in mice that received 78% of their daily calorie intake as fat in an essentially carbohydrate-free diet formulation 154 . Follow-up studies have shown that the proposed effect of HFD consumption on gut barrier integrity largely depends on housing conditions and bacterial colonization of the gut 155,156 .…”

Section: Dio-sensitive Sprague Dawleymentioning

confidence: 99%

Animal models of obesity and diabetes mellitus

et al. 2018

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More than one-third of the worldwide population is overweight or obese and therefore at risk of developing type 2 diabetes mellitus. In order to mitigate this pandemic, safer and more potent therapeutics are urgently required. This necessitates the continued use of animal models to discover, validate and optimize novel therapeutics for their safe use in humans. In order to improve the transition from bench to bedside, researchers must not only carefully select the appropriate model but also draw the right conclusions. In this Review, we consolidate the key information on the currently available animal models of obesity and diabetes and highlight the advantages, limitations and important caveats of each of these models.

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“…The recently established catalogue of the mouse gut metagenome shows that the environment, determined by mouse provider and housing laboratory, has a more pronounced effect on the conventional gut microbiota than seen for mouse strain, feed or gender (Xiao et al, 2015). Environmental reprogramming of microbiota by breeding mice in a new site (Ussar et al, 2015) or altering housing conditions (Müller et al, 2015) is shown to change the development of the metabolic syndrome. However, the extent of microbial spread within a specific environment and the effects of this spread on the development of metabolic phenotypes have currently not been studied.…”

Section: Introductionmentioning

confidence: 99%

Environmental spread of microbes impacts the development of metabolic phenotypes in mice transplanted with microbial communities from humans

et al. 2016

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Microbiota transplantation to germ-free animals is a powerful method to study involvement of gut microbes in the aetiology of metabolic syndrome. Owing to large interpersonal variability in gut microbiota, studies with broad coverage of donors are needed to elucidate the establishment of human-derived microbiotas in mice, factors affecting this process and resulting impact on metabolic health. We thus transplanted faecal microbiotas from humans (16 obese and 16 controls) separately into 64 germ-free Swiss Webster mice caged in pairs within four isolators, with two isolators assigned to each phenotype, thereby allowing us to explore the extent of microbial spread between cages in a well-controlled environment. Despite high group-wise similarity between obese and control human microbiotas, transplanted mice in the four isolators developed distinct gut bacterial composition and activity, body mass gain, and insulin resistance. Spread of microbes between cages within isolators interacted with establishment of the transplanted microbiotas in mice, and contributed to the transmission of metabolic phenotypes. Our findings highlight the impact of donor variability and reveal that inter-individual spread of microbes contributes to the development of metabolic traits. This is of major importance for design of animal studies, and indicates that environmental transfer of microbes between individuals may affect host metabolic traits.

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Gut barrier impairment by high‐fat diet in mice depends on housing conditions

Abstract: We demonstrated that housing conditions and associated changes in gut bacterial colonization are pivotal for maintenance of gut barrier integrity in DIO mice.

Cited by 57 publications

References 39 publications

Prolonged transfer of feces from the lean mice modulates gut microbiota in obese mice

Prolonged transfer of feces from the lean mice modulates gut microbiota in obese mice

Animal models of obesity and diabetes mellitus

Environmental spread of microbes impacts the development of metabolic phenotypes in mice transplanted with microbial communities from humans

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