Gut Microbial Signatures Associated with Peanut Allergy in a BALB/c Mouse Model

Abstract

Multiple studies have uncovered the pivotal role of gut microbiota in the development of food allergy. However, the effects of gut microbiota on peanut allergy are still unclear. Here, we characterized the gut microbiota composition of peanut-allergic mice by 16S rRNA sequencing and analyzed the correlation between allergic indicators and gut microbiota composition. Outcomes showed that the gut microbiota composition was reshaped in peanut-allergic mice, with Acidobacteriota, Lachnospiraceae, Rikenellaceae, Alistipes, Lachnospiraceae_NK4A136_group significantly down-regulated and Muribaculaceae up-regulated. All of them were significantly correlated with the serum peanut-specific antibodies. These results suggested that these six bacterial OTUs might be the gut microbial signatures associated with peanut allergy.

β-Sitosterol protects against food allergic response in BALB/c mice by regulating the intestinal barrier function and reconstructing the gut microbiota structure

“…62 It has also been discovered that the relative abundance of Acidobacteriota and Lachnospiraceae was reduced in the intestine of peanut-allergic mice. 63 This study found a remarkable decline in the proportion of Firmicutes in OVA-sensitized mice, which was consistent with previous findings. 20 There was also a noticeable increase in the relative abundance of Desulfobacterota .…”

Effects of hazelnut protein isolate-induced food allergy on the gut microenvironment in a BALB/c mouse model

“…63,64 In agreement with our results, Rachid et al 65 reported that there was increased relative abundance of Bacteroidetes and reduced relative abundances of Firmicutes and Proteobacteria in food-allergic infants. The relative abundance of Acidobacteriota was remarkably decreased in peanut-induced allergic mice, 22 which was consistent with our observations.…”

“…63,64 In agreement with our results, Rachid et al 65 reported that there was increased relative abundance of Bacteroidetes and reduced relative abundances of Firmicutes and Proteobacteria in food-allergic infants. The relative abundance of Acidobacteriota was remarkably decreased in peanut-induced allergic mice, 22 which was consistent with our observations. LEfSe analysis illustrated that at the genus level, upregulated relative abundances of Bacteroides, Lactobacillus, Parabacteroides, Alloprevotella and Helicobacter were observed in the HS group.…”

“…73 It was also shown in the BALB/c mouse model that the Lactobacillus was noticeably accumulated in OVA or peanut sensitized mice. 22,74 In general, all of the variations in the gut microbiota affected by HPI sensitization resulted in gut microbiota dysbiosis, contributing to FA development. 30…”

“…21 The sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis of HPI was performed according to our previous study. 22…”