Gut microbiota-derived metabolites contribute negatively to hindgut barrier function development at the early weaning goat model

Zhang, Ke; Xu, Yangbin; Yang, Yuxin; Guo, Mengmeng; Zhang, Ting; Zong, Bo; Huang, Shuhong; Suo, Langda; Ma, Baohua; Wang, Xiaolong; Wu, Yaoyao; Brügger, Daniel; Chen, Yulin

doi:10.1016/j.aninu.2022.04.004

Cited by 16 publications

(16 citation statements)

References 68 publications

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“…However, it can also play a detrimental role in uremic toxicity induced by antibiotics and chemotherapy by producing indole sulfate ( 43 ). Similarly, B. acidifaciens negatively contributes to gut barrier disruption by inducing a decrease in the expression of zonulin-1 and claudin-1 ( 44 ). Thus, depending on the context, B. acidifaciens can exert divergent roles.…”

Section: Discussionmentioning

confidence: 99%

Gut epithelial barrier dysfunction in lupus triggers a differential humoral response against gut commensals

et al. 2023

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IntroductionSystemic lupus erythematosus is an autoimmune disease with multisystemic involvement including intestinal inflammation. Lupus-associated intestinal inflammation may alter the mucosal barrier where millions of commensals have a dynamic and selective interaction with the host immune system. Here, we investigated the consequences of the intestinal inflammation in a TLR7-mediated lupus model.MethodsIgA humoral and cellular response in the gut was measured. The barrier function of the gut epithelial layer was characterised. Also, microbiota composition in the fecal matter was analysed as well as the systemic humoral response to differential commensals.ResultsThe lupus-associated intestinal inflammation modifies the IgA+ B cell response in the gut-associated lymphoid tissue in association with dysbiosis. Intestinal inflammation alters the tight junction protein distribution in the epithelial barrier, which correlated with increased permeability of the intestinal barrier and changes in the microbiota composition. This permeability resulted in a differential humoral response against intestinal commensals.DiscussionLupus development can cause alterations in microbiota composition, allowing specific species to colonize only the lupus gut. Eventually, these alterations and the changes in gut permeability induced by intestinal inflammation could lead to bacterial translocation.

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Section: Discussionmentioning

confidence: 99%

Gut epithelial barrier dysfunction in lupus triggers a differential humoral response against gut commensals

et al. 2023

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“…The supernatants were then stored in 2-mL screw-cap vials. An Agilent 7820A gas chromatograph (Agilent Technologies, Santa Clara, USA) was used to measure the SCFA concentrations in the samples ( 69 ).…”

Section: Methodsmentioning

confidence: 99%

Gut Microbiota and Acylcarnitine Metabolites Connect the Beneficial Association between Estrogen and Lipid Metabolism Disorders in Ovariectomized Mice

Guo¹,

Cao

et al. 2023

Microbiol Spectr

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“…The 2 mL screwcap vials used to store the supernatants. The Agilent 7820A gas chromatograph (Agilent Technologies, Santa Clara, USA) was used to measure the SCFA concentrations in content samples from the cecum [35].…”

Section: Analysis Of Cecum Content Scfasmentioning

confidence: 99%

“…The effectiveness of the isolated DNA was evaluated using an electrophoresis on 1% agarose gel. The V3-V4 region of the DNA was ampli ed using the primers 338F (5'-ACTCCTACGGGAGGCAGCAG-3') and 806R (5'-GGACTACHVGGGTWTCTAAT-3') on a thermocycler PCR machine (Gene Amp 9700, ABI, USA) [35]. Puri ed amplicons were pooled in equimolar ratios and subjected to paired-end sequencing (2×300 bp) using an Illumina MiSeq platform (Illumina, San Diego, California, CA, USA) using Major Biobio Technology Co. Ltd. standard protocols (Major, Shanghai, China).…”

Section: Caecal Bacterial Community Determination and Analysismentioning

confidence: 99%

Gut microbiota and acylcarnitine connect the beneficial association between estrogen and lipid metabolism disorders in ovariectomized mice

Zhang

Guo

et al. 2022

Preprint

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Decreased estrogen levels are one of the main causes of lipid metabolism disorders and coronary heart disease in women after menopause. Exogenous estradiol benzoate is effective to some extent in alleviating lipid metabolism disorders caused by estrogen deficiency, but the role of gut microbes in the regulation process is not yet clear. The objective of this 45 days randomized trial was to investigate the effects of estradiol benzoate supplementation on lipid metabolism, gut microbiota and metabolites in ovariectomized (OVX) mice, and revealing the important role of gut microbes and metabolites in the regulation of lipid metabolism disorders. This study found that high doses of estradiol benzoate supplementation effectively attenuated fat accumulation in OVX mice and significantly altered the expression of genes enriched in hepatic cholesterol metabolism and unsaturated fatty acid metabolism pathways. Further screening of the gut for characteristic metabolites associated with improved lipid metabolism disorders revealed that estradiol benzoate supplementation influences major subsets of acylcarnitine metabolites, and ovariectomy significantly increased the abundance of characteristic microbes that were significantly negatively associated with acylcarnitine synthesis, including Lactobacillus and Eubacterium_ruminantium_group bacteria, while estradiol benzoate supplementation significantly increased the abundance of characteristic microbes that were significantly positively associated with acylcarnitine synthesis, including Ileibacterium and Bifidobacterium bacteria. The use of pseudo-sterile mice gut microbial deficiency greatly facilitates the synthesis of acylcarnitine due to estradiol benzoate supplementation and alleviates lipid metabolism disorders to a greater extent in OVX mice. Our findings established a role for gut microbes in the progression of estrogen deficiency-induced lipid metabolism disorders, and screened for key target bacteria that may have the potential to regulate acylcarnitine synthesis. These findings suggest a possible route for the use of microbe or acylcarnitine to regulate estrogen deficiency-induced disorders of lipid metabolism.

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Gut microbiota-derived metabolites contribute negatively to hindgut barrier function development at the early weaning goat model

Cited by 16 publications

References 68 publications

Gut epithelial barrier dysfunction in lupus triggers a differential humoral response against gut commensals

Gut epithelial barrier dysfunction in lupus triggers a differential humoral response against gut commensals

Gut Microbiota and Acylcarnitine Metabolites Connect the Beneficial Association between Estrogen and Lipid Metabolism Disorders in Ovariectomized Mice

Gut microbiota and acylcarnitine connect the beneficial association between estrogen and lipid metabolism disorders in ovariectomized mice

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