Gut mucosa in the rats exposed temporary mechanical obstruction fed with probiotic

Abstract

BACKGROUND: Created a model in the rats, to prevent mucosal damage and related effects in the patients, who were operated due to mechanical obstruction. Some groups fed fodder with probiotics, some groups fed with standard fodder. It is objected that the damage of gut mucosa and related effects on how to expose the differences of the groups. METHODS:In this study, 48 female Wistar-albino type rats are separated into five groups randomly. In the first operation, rats' terminal ileum was tied up with silk except for the control group. Two groups 24, the other two groups 48 hours later operated again and terminal ileum obstructions were removed. During that time, each one of those 24 and 48 hours of obstructed groups were fed with probiotic. Twenty-four hours later, the control group and other groups were operated for the third time for sampling. Terminal ileum, liver, spleen, MLN (Mesenteric lymph node) and blood samples were taken. RESULTS:The research group, which was obstructed and fed with probiotics during 48 hours, was significantly observed in increased mucosa cell loss and mucosal edema. Bacterial translocation was found more common in groups without probiotics. Tissue GR (Glutathione reductase) and erythrocyte CAT (Catalase) were lower in the group of 24 hours obstructed and given probiotics. CONCLUSION:The findings suggest that the high rate of mucosal edemas in the groups that are fed with probiotics can be seen as damage, but we think that probiotics are consonant with the strength of the mucosal barrier. Thus, in the groups fed with probiotics, it is possible that bacterial translocation is seen less, and some antioxidative enzymes are found less. Further studies are needed to investigate the benefits of probiotics in patients operated for obstruction.

Experimental Animal Models for Studying Intestinal Obstruction

“…Ligation models: A simple ligation of the lumen at the desired level of obstruction has been a commonly cited technique since the beginning of the twentieth century [26][27][28][56][57][58][59][60][61][62][63][64][65][66][67][68][69][70][71][72][73] and is probably the most feasible and cost-effective model to produce an acute complete intestinal obstruction. The desired intestinal loop is identified after entering the abdominal cavity, and the adjacent mesentery is carefully dissected so as to create a small mesenteric window but not to damage the mesentery itself or the mesenteric marginal vessels.…”