Gut mycobiome dysbiosis in rats showing retinal changes indicative of diabetic retinopathy

Padakandla, Shalem Raj; Das, Taraprasad; Prashanthi, Gumpili Sai; Angadi, Kiran Kumar; Reddy, S. Sreenivasa; Reddy, G. Bhanuprakash; Shivaji, Sisinthy

doi:10.1371/journal.pone.0267080

Cited by 2 publications

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References 84 publications

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“…Our preclinical murine model has identified changes in the microbiota of diabetic and healthy rats. We detected overlaps between diabetic rats with and without DR [26,27]. Further, we have also noted dysbiosis (measured as abundance and diversities) of both bacteria (gut microbiome) and fungi (gut mycobiome) in people with DM with and without DR, compared with the healthy controls [7,8,19].…”

Section: Introductionmentioning

confidence: 59%

Intraocular Microbiome in Diabetes and Diabetic Retinopathy: A Pilot Study

et al. 2023

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Introduction:The objective of this study was to compare the microbiome in the aqueous humour and gut of people with diabetes mellitus (DM) with and without diabetic retinopathy (DR). Methods: This was a prospective controlled study. The study included 17 people undergoing intraocular surgery in their naı ¨ve eyes. Stool samples were obtained in the perioperative period; aqueous humour samples of sufficient quantity were obtained in 12 people during intraocular surgery. Dietary information was obtained using a previously validated questionnaire. The gut and aqueous humour samples were assessed for microbiome using 16S rRNA gene sequencing coupled with QIIME and R software. Results: Aqueous humour was analysed in 12 people: 4 each healthy controls, people with DM, and people with DR. There were minor differences at the phyla levels, but the aqueous humour microbiomes of healthy controls, DM, and DR formed three distinct clusters on heat map analysis with discriminatory genera. This genera-level clustering was more apparent for the intraocular than the gut microbiome. In people with DM and DR, we identified genera unique to the eye or the gut. There was a consistent reduction in the abundance of anti-inflammatory bacteria in people with DR than DM. Conclusions: There is a difference in intraocular and gut microbiome regardless of disease or health. Our preliminary findings indicate distinctive features of the intraocular microbiome in people with DR compared with those without

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