Gynaecological Cytology

Boon, Mathilde E.; Tabbers-Boumeester, Mette Lise

doi:10.1007/978-1-349-16336-6

Cited by 13 publications

(3 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Naked nuclei, classified by Boon et al11 as reserve cells, demonstrated no immunoreactivity with either antibody (Fig. 2c).…”

Section: Resultsmentioning

confidence: 76%

“…Small cells with relatively large nuclei and a thin cytoplasmic rim that displayed beginning squamoid characteristics could be identified as reserve cells or very immature squamous cells on the basis of their keratin 17 expression. Naked nuclei, identified by Boon as reserve cells,11 displayed no immunoreactivity at all. The cytoplasm of these cells was undoubtedly stripped, and this could of course explain their lack of immunoreactivity.…”

Section: Discussionmentioning

confidence: 98%

See 1 more Smart Citation

Can keratin 8 and 17 immunohistochemistry be of diagnostic value in cervical cytology?

Martens

Baars

Smedts³

et al. 1999

Cancer

View full text Add to dashboard Cite

BACKGROUND Based on results from evaluation of tissue sections from premalignant lesions of the uterine cervix, the authors examined the hypothesis that immunostaining of Papanicolaou‐stained cytologic smears with monoclonal antibodies to keratins 8 and 17 allows detection of cervical intraepithelial neoplasia (CIN) with progressive potential. They also investigated whether detection of these two keratin subtypes could be of help in the analysis of normal and/or poor quality cytology smears. METHODS Sixty‐one Papanicolaou‐stained smears, representing 25 normal smears, 8 CIN 1, 7 CIN 2, 18 CIN 3, and 3 cervical carcinomas, were stained with CAM 5.2 and E3, which are capable of detecting keratin 8 and 17, respectively. The percentages of immunoreactive normal, metaplastic, dysplastic, and malignant epithelial cells were determined. RESULTS In normal cervical smears, keratin 8 was detected in endocervical columnar cells and sporadically in immature squamous metaplastic cells. Keratin 17 was identified in reserve cells and frequently in immature squamous metaplasic cells. In CIN, the number of cases in which keratin 8 was present increased with the severity of the lesion. Keratin 17 was found in the majority of CIN lesions, irrespective of grade. Intensity of immunostaining and number of cells stained per lesion varied and were also not related to the severity of CIN. CONCLUSIONS The use of the keratin 8 antibody in normal cervical smears enabled the detection of endocervical cells in cases where they were thought to be absent, particularly in cases with severe inflammation. Staining with keratin 17 enabled the identification of reserve cells or immature metaplastic cells, which were often misinterpreted as parabasal cells. The application of antibodies to these subtypes of keratins in cervical cytology can to a certain extent help in the identification of CIN and may in future be tested in automated screening. Cancer (Cancer Cytopathol) 1999;87:87–92. © 1999 American Cancer Society.

show abstract

“…Naked nuclei, classified by Boon et al11 as reserve cells, demonstrated no immunoreactivity with either antibody (Fig. 2c).…”

Section: Resultsmentioning

confidence: 76%

Section: Discussionmentioning

confidence: 98%

Can keratin 8 and 17 immunohistochemistry be of diagnostic value in cervical cytology?

Martens

Baars

Smedts³

et al. 1999

Cancer

View full text Add to dashboard Cite

show abstract

“…In the Netherlands, in the 1970s it was the accepted approach not to treat LSIL, and in these years, follow-up showed that of 2,600 women with a smear diagnosed as LSIL, only three developed invasive carcinoma. 17 Secondly, the time it takes for a low-grade lesion to develop into invasive carcinoma should be considered. The estimated mean duration of progression of preclinical lesions is 10 -16 yr. 16,18 For the Netherlands, this implies that with the current 5-yr interval population screening program, the woman has three subsequent chances that a lesion will be detected.…”

Section: Discussionmentioning

confidence: 99%

Neural network‐based screening (NNS) in cervical cytology: No need for the light microscope?

Kok

Schouw

Boon

et al. 2001

Diagnostic Cytopathology

Self Cite

View full text Add to dashboard Cite

Neural network-based screening (NNS) of cervical smears can be performed as a so-called "hybrid screening method," in which parts of the cases are additionally studied by light microscope, and it can also be used as "pure" NNS, in which the cytological diagnosis is based only on the digital images, generated by the NNS system. A random enriched sample of 985 cases, in a previous study diagnosed by hybrid NNS, was drawn to be screened by pure NNS. This study population comprised 192 women with (pre)neoplasia of the cervix, and 793 negative cases. With pure NNS, more cases were recognized as severely abnormal; with hybrid NNS, more cases were cytologically diagnosed as low-grade. For a threshold value > or = HSIL (high-grade squamous intraepithelial lesions), the areas under the receiver operating characteristic (ROC) curves (AUC) were 81% (95% CI, 75-88%) for pure NNS vs. 78% (95% CI, 75-81%) for hybrid NNS. For low-grade squamous intraepithelial lesions (LSIL), the AUC was significantly higher for hybrid NNS (81%; 95% CI, 77-85%) than for pure NNS (75%; 95% CI, 70-80%). Pure NNS provides optimized prediction of HSIL cases or negative outcome. For the detection of LSIL, light microscopy has additional value.

show abstract

Adenocarcinomain situ of the cervix: An underdiagnosed lesion

Boon

Baak

Kurver

et al. 1981

Cancer

117

View full text Add to dashboard Cite

Although invasive adenocarcinoma of the cervix constitutes 5--15% of all cervical cancers, the in situ counterpart is underrepresented in the published series of percursor lesions of cervical cancer. Moreover, no cases are known to have been published in which in situ adenocarcinoma preceded invasive cancer. Partly, this can be explained by the fact that in situ adenocarcinoma is an underdiagnosed lesion. In a series of 52 cases of adenocarcinoma of the uterine cervix, 18 "negative" endocervical biopsies, taken 3--7 years prior to the clinical presentation of cancer, were available for study. In five of these cases, areas of adenocarcinoma in situ were found. The quantitative parameters of these "missed" adenocarcinomas in situ and adenocarcinomas in situ adjacent to invasive cancer were the same. The in situ lesions differed significantly from benign endocervical epithelium. This study strongly suggests that these lesions may progress to invasive cancer. With the acquired information on the quantitative features of adenocarcinoma in situ cells, the most significant criteria for routine diagnostic practice can be identified.

show abstract

Gynaecological Cytology

Cited by 13 publications

References 0 publications

Can keratin 8 and 17 immunohistochemistry be of diagnostic value in cervical cytology?

Can keratin 8 and 17 immunohistochemistry be of diagnostic value in cervical cytology?

Neural network‐based screening (NNS) in cervical cytology: No need for the light microscope?

Adenocarcinomain situ of the cervix: An underdiagnosed lesion

Contact Info

Product

Resources

About