Gynogenic plant regeneration from unpollinated flower explants of Eragrostis tef (Zuccagni) Trotter

Gugsa, Likyelesh; Sarial, A. K.; Lörz, Horst; Kumlehn, Jochen

doi:10.1007/s00299-006-0200-z

Cited by 31 publications

(29 citation statements)

References 19 publications

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“…However, this effect is likely to be genotype-dependent since we did not observe any beneficial effect of thermal shock pretreatment on the ELS formation of the five Thai cultivars. Insensitivity to thermal shock pretreatment on several plants has also been reported (Metwally et al, 1998;Gugsa et al, 2006). It was shown that squash ovules without cold pretreatment at 4°C produced a better embryogenic response than the ones treated at 4°C for 2, 4 or 8 days (Metwally et al, 1998).…”

Section: Discussionmentioning

confidence: 84%

“…It was shown that squash ovules without cold pretreatment at 4°C produced a better embryogenic response than the ones treated at 4°C for 2, 4 or 8 days (Metwally et al, 1998). Similarly, pretreatment at 4°C for up to 9 days or at 32°C for 1 day did not improve gynogenic development of tef pistil culture (Gugsa et al, 2006). ELS and callus formation potentials varied significantly when using different induction media.…”

Section: Discussionmentioning

confidence: 97%

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Evaluation of Factors Affecting Embryo-like Structure and Callus Formation in Unpollinated Ovary Culture of Cucumber (Cucumis sativus)

Tantasawat¹,

Sorntip²,

Poolsawat³

et al. 2015

IJAB

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To cite this paper: Tantasawat, P.A., A. Sorntip, O. Poolsawat, W. Chaowiset and P. Pornbungkerd, 2015. Evaluation of factors affecting embryo-like structure and callus formation in unpollinated ovary culture of cucumber (Cucumis sativus AbstractThe effects of various factors including genotypes of donor plants, induction and differentiation media, and thermal shock pretreatment were evaluated on embryo-like structure (ELS) and callus formation in an unpollinated ovary culture of cucumber. All five cucumber cultivars, which were used as donor plants, produced ELSs and calli, although their ELS and callus formation potentials varied significantly. The addition of 1 mg/L thidiazuron (TDZ) and 1 mg/L 6-benzylaminopurine (BA) into the induction medium resulted in the highest percentage of ELS formation, ranging from 42.3 to 91.4% with an average of 60.4%. However, the highest percentage of callus formation was observed in an induction medium containing 2 mg/L BA, 0.5 mg/L indole-3-acetic acid (IAA), 1 mg/L gibberellic acid (GA 3 ) and 32 mg/L putrescine (70.8%). By contrast, differentiation media had no significant effect on the formation potentials of both ELSs and calli. Thermal shock pretreatment reduced the percentage of ELS formation ca. 1.3-fold, but had no significant effect on callus formation. These results can be implicated for the efficient production of cucumber doubled haploids in the future.

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Section: Discussionmentioning

confidence: 84%

Section: Discussionmentioning

confidence: 97%

Evaluation of Factors Affecting Embryo-like Structure and Callus Formation in Unpollinated Ovary Culture of Cucumber (Cucumis sativus)

Tantasawat¹,

Sorntip²,

Poolsawat³

et al. 2015

IJAB

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“…Variation from gynogenically derived tef (Eragrostis teff (Zuccagni)), an important cereal crop of Ethiopia, was found for plant height, panicle length, culm thickness, seed size, and maturity (Gugsa and Loerz, 2013;Gugsa et al, 2006). Likewise, several DH regenerants obtained after anther culture (or microspores) showed large variation for improved agronomic characteristics in rice (Ying et al, 1996;Yoshida et al, 1998), while dihaploid plants in potato originating through gametoclonal variation during androgenic dihaploidization were genetically most distinct from their tetraploid anther-derived sibs as well as anther donor (Sarkar et al, 2010).…”

Section: Gametoclonal Variationmentioning

confidence: 99%

Haploids: Constraints and opportunities in plant breeding

Dwivedi

Britt

Tripathi

et al. 2015

Biotechnology Advances

235

134

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The discovery of haploids in higher plants led to the use of doubled haploid (DH) technology in plant breeding. This article provides the state of the art on DH technology including the induction and identification of haploids, what factors influence haploid induction, molecular basis of microspore embryogenesis, the genetics underpinnings of haploid induction and its use in plant breeding, particularly to fix traits and unlock genetic variation. Both in vitro and in vivo methods have been used to induce haploids that are thereafter chromosome doubled to produce DH. Various heritable factors contribute to the successful induction of haploids, whose genetics is that of a quantitative trait. Genomic regions associated with in vitro and in vivo DH production were noted in various crops with the aid of DNA markers. It seems that F2 plants are the most suitable for the induction of DH lines than F1 plants. Identifying putative haploids is a key issue in haploid breeding. DH technology in Brassicas and cereals, such as barley, maize, rice, rye and wheat, has been improved and used routinely in cultivar development, while in other food staples such as pulses and root crops the technology has not reached to the stage leading to its application in plant breeding. The centromere-mediated haploid induction system has been used in Arabidopsis, but not yet in crops. Most food staples are derived from genomic resources-rich crops, including those with sequenced reference genomes. The integration of genomic resources with DH technology provides new opportunities for the improving selection methods, maximizing selection gains and accelerate cultivar development. Marker-aided breeding and DH technology have been used to improve host plant resistance in barley, rice, and wheat. Multinational seed companies are using DH technology in large-scale production of inbred lines for further development of hybrid cultivars, particularly in maize. The public sector provides support to national programs or small-medium private seed for the exploitation of DH technology in plant breeding.

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“…This technique is the female equivalent of the process described in the paragraph above, and has been applied to species including sugar beet [55,56], onion [57,58], squash [59], gerbera [60], rice [61], maize [62], niger [63] and tea [64]. Ovules have also been used as a transformation target [39].…”

Section: Ovule Culturementioning

confidence: 99%

Plant Cell Culture – Present and Future

Dunwell

2010

Plant Cell Culture

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Plant cell culture has a long history of development and exploitation that has been reviewed many times [1]. There are also several monographs and textbooks that provide detailed discussion on specific techniques and their application [2, 3]. By necessity therefore, the present review is limited in scope and, for the most part, will focus on recent publications. The various sections below each concentrate on a specific aspect of the technology; these are followed by a summary section relating to commercialization. MicropropagationMicropropagation in vitro is well established as a method to propagate [4], preserve and transport germplasm of many species including horticultural [5-7], medicinal [8, 9] and woody [10,11] plants. It also reduces the risk of moving pathogens and insects with the germplasm owing to the inherent pathogen detection capabilities of aseptic cultures. Since this technology is usually limited to the multiplication of pre-existing meristems and does not involve the regeneration of plants from single cells or tissue, it will not be discussed in detail here.

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Gynogenic plant regeneration from unpollinated flower explants of Eragrostis tef (Zuccagni) Trotter

Cited by 31 publications

References 19 publications

Evaluation of Factors Affecting Embryo-like Structure and Callus Formation in Unpollinated Ovary Culture of Cucumber (Cucumis sativus)

Evaluation of Factors Affecting Embryo-like Structure and Callus Formation in Unpollinated Ovary Culture of Cucumber (Cucumis sativus)

Haploids: Constraints and opportunities in plant breeding

Plant Cell Culture – Present and Future

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