Evaluation of Factors Affecting Embryo-like Structure and Callus Formation in Unpollinated Ovary Culture of Cucumber (Cucumis sativus)

Tantasawat, Piyada Alisha; Sorntip, A.; Poolsawat, O.; Chaowiset, W.; Pornbungkerd, Paniti

doi:10.17957/ijab.17.3.14.257

Cited by 25 publications

(17 citation statements)

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“…Previously, we evaluated factors influencing the induction of ELSs and calli in unpollinated ovary culture of five Thai cucumber cultivars and found that donor plant genotypes and induction media, differing in PGRs, affected both ELS and callus formation. The addition of TDZ and 6-benzylaminopurine (BAP) into the induction medium resulted in the highest percentage of ELS formation (Tantasawat et al, 2015). Similarly, the addition of TDZ increased embryogenesis success in ovary/ovule culture in other studies (Li et al, 2013;Moqbeli et al, 2013;Suprunova and Shmykova, 2008).…”

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confidence: 83%

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Effects of Exogenous Application of Plant Growth Regulators on Growth, Yield, and In Vitro Gynogenesis in Cucumber

Tantasawat

Sorntip

Pornbungkerd

2015

horts

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The effects of exogenous application of plant growth regulators (PGRs) were evaluated on both field performance and in vitro gynogenesis of ‘Chai Lai’ and ‘Big C’ cucumber (Cucumis sativus L.). Plants were sprayed with two concentrations of N6-furfuryladenine [kinetin (KIN); 2 and 20 ppm], 2,3,5-triiodobenzoic acid (TIBA; 1 and 10 ppm), naphthaleneacetic acid (NAA; 10 and 100 ppm), abscisic acid (ABA; 2 and 20 ppm), thidiazuron (TDZ; 1 and 10 ppm), and maleic hydrazide (MH; 10 and 100 ppm) to assess their effects on vegetative growth and floral and yield related traits in the Winter of 2013 and in the Summer of 2014 compared with distilled water control. Meanwhile, the effects of two PGRs (KIN and TIBA) on cucumber gynogenesis were also investigated in vitro. Growth parameters and floral and yield-related traits were significantly affected by the various PGRs in both cultivars during both seasons. In both cultivars, the highest yield was obtained with the application of 10 ppm NAA during the Winter of 2013 (1.5- to 1.8-fold over control) and with 1 ppm TIBA during the Summer of 2014 (2.1- to 2.2-fold over control). With regard to the ovary culture response, exogenous application of KIN and TIBA on floral buds tended to enhance callus formation in ‘Chai Lai’ cultured on I7 medium, whereas no effect was observed in ‘Big C’. The embryo-like structure (ELS) formation efficiencies also tended to increase with 2 and 20 ppm KIN and 1 ppm TIBA application in ‘Chai Lai’ and with 20 ppm KIN and 1 ppm TIBA application in ‘Big C’ when cultured on I7 medium. Nevertheless, TIBA at high concentrations (10 ppm) decreased the percentages of ELS formation and the number of ELSs/piece in both cultivars. These results suggest that the polar auxin transport may play a major role on growth, floral and yield-related traits, yield as well as in vitro gynogenesis in cucumber. However, the success of exogenous applications of these PGRs depended on several factors including plant genotypes, growing seasons, types and concentrations of PGRs, and for ovary culture, the responses also varied according to the induction media used. Chemical names: abscisic acid (ABA); maleic hydrazide (MH); naphthaleneacetic acid (NAA); N6-furfuryladenine (kinetin; KIN); thidiazuron (TDZ); 2,3,5-triiodobenzoic acid (TIBA).

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confidence: 83%

“…Unpollinated ovary culture. Pistillate flowers, 12 h before anthesis, were obtained from donor cucumber plants, and unpollinated ovaries were sterilized according to Tantasawat et al (2015). Longitudinal sliced ovaries were placed on three different induction media.…”

Section: Methodsmentioning

confidence: 99%

Effects of Exogenous Application of Plant Growth Regulators on Growth, Yield, and In Vitro Gynogenesis in Cucumber

Tantasawat

Sorntip

Pornbungkerd

2015

horts

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“…Despite these difficulties, to inducing gynogenesis in vitro, especially when the haploid induced by the male gametophyte is not successful or the induction rate is too low and when self-incompatibility, male-sterile and dioecious plants occurs, is an effective way to obtain haploid or double haploid plants [3]. In vitro gynogenesis is mainly used in the Cucurbitaceae, and previous studies have revealed that this technique is still imperfect and that the embryogenesis rate is low [4][5][6][7][8][9][10][11]. The study of the mechanism of embryogenesis has therefore been delayed, and the understanding of the mechanisms underlying gynogenesis induction is extremely limited.…”

Section: Introductionmentioning

confidence: 99%

Analysis of differentially expressed genes during embryogenesis in ovary culture of cucumber (Cucumis sativus L.)

Deng¹,

Fu²,

Tang³

et al. 2019

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Background: Ovary culture has been a useful way to generate double haploid (DH) plant in cucumber (Cucumis sativus L.). However, the rate of embryo induction is low, and the probability for the induced embryo to grow into normal embryo is low as well. This is largely due to unknown of the mechanism of embryogenesis in cucumber. In this study, the differentially expressed genes during embryogenesis, including the early stages of embryo formation, embryo maturation and shoot formation, was investigated with transcriptomic technique to set up basis for a more efficient ovary culture technology Results: Cytological observations led to suggestions that cell enlargement is the symbol that gametophytes had switched to the sporophyte development pathway during the early embryogenesis stage. In this stage, RNA-seq revealed 3468 up-regulated genes, including hormone signal transduction genes, hormone response genes and stress-induced genes. The reported embryogenesis-related genes BBM, HSP90 and AGL were also actively expressed during this stage. The total of 480 genes that function in protein complex binding, microtubule binding, tetrapyrrole binding, tubulin binding and other microtubule activities were continuously up-regulated during the embryo maturation stage, indicating that the cytoskeleton structure was continuously being built and maintained by the action of microtubule-binding proteins and enzyme modification during embryo development. In the shoot formation stage, 1383 genes were up-regulated, which were mainly enriched in phenylpropanoid biosynthesis, plant hormone signal transduction, phenylalanine metabolism, and starch and sucrose metabolism. The shoot formation stage might be regulated by 6 transcription factors that contained a B3 domain, 9 genes in the AP2/ERF family and 2 genes encoded WUS homologous domain proteins. Conclusions: Findings from this study offer a valuable framework for explaining the transcriptional regulatory mechanism underlying embryogenesis in cucumber ovary culture.

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“…Diao et al, 2009;Moqbeli et al, 2013;Tantasawat et al, 2015;Sorntip et al, 2017) Suprunova, Shmykova (2008). использовали в своих исследованиях среду МСм (Masuda et al, 1981).…”

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Production of Doubled Haploids in cucumber

et al. 2019

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Implementation of cell technologies has essentially improved the plant breeding process in agricultural crops in the world. The production of pure lines in cultivated crops, particularly among cross-pollinated species such as cucumber (Cucumis sativus L.) requires much time, labor and expense. Thus, the use of DH-plants for production of fully homozygous lines for one year becomes a very promising method for near cucumber breeding program. The major factor limiting the wide use of DH is a lack of effective protocol for large-scale plant production. In this review the historical facts with description of three main methods of DH-plant production were presented. By now these three methods have been such as parthenogenesis in situ induced by pollination with irradiated or chemically treated pollen; androgenesis in vitro including anther and isolated microspore cultivation in vitro; gynogenesis through ovule cultivation in vitro. Comparative analysis of published data with regard to the efficiency of the technology for DH-plant production was shown as well as advantages and limitations of each technology were described.

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Evaluation of Factors Affecting Embryo-like Structure and Callus Formation in Unpollinated Ovary Culture of Cucumber (Cucumis sativus)

Cited by 25 publications

References 20 publications

Effects of Exogenous Application of Plant Growth Regulators on Growth, Yield, and In Vitro Gynogenesis in Cucumber

Effects of Exogenous Application of Plant Growth Regulators on Growth, Yield, and In Vitro Gynogenesis in Cucumber

Analysis of differentially expressed genes during embryogenesis in ovary culture of cucumber (Cucumis sativus L.)

Production of Doubled Haploids in cucumber

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