H ANTIGEN OF<i>SALMONELLA TYPHOSA</i>

Tully, Joseph G.; Gaines, Sidney

doi:10.1128/jb.81.6.924-932.1961

Cited by 6 publications

(3 citation statements)

References 14 publications

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“…Mouse protection tests with viable and AKD typhoid vaccines confirmed results of a previous study (Tully and Gaines, 1961a). Mice can be protected against a virulent challenge with non-Vi typhoid vaccines but, as shown by this study and the findings of Standfast (1960a), very large numbers of the W-form cells are required.…”

Section: Discussionsupporting

confidence: 89%

“…Studies in our laboratory (Gaines, Tully, and Tigertt, 1961;Tully and Gaines, 1961a) involved examination of variants recovered from the brains of mice after intracerebral inoculation with selected S. typhosa strains. In studies where purified Vi antigen had been added to an intracerebral inoculum of the Ty2W strain, we were unsuccessful in recovering from mouse brain typhoid strains which had reverted to the V form; all cultures conformed to the serological characteristics of the original Ty2W strain.…”

Section: Discussionmentioning

confidence: 99%

“…Virulence tests. Procedures for intraperitoneal and intracerebral virulence tests were identical to those previously described (Tully and Gaines, 1961a;, with the exception that only C3H/He mice (obtained from Microbiological Associates, Bethesda, Md.) were employed.…”

Section: Methodsmentioning

confidence: 99%

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VI-NEGATIVE STRAINS OFSALMONELLA TYPHOSA: ATTEMPTS TO INDUCE W-V REVERSION AND THE USE OF NON-VI STRAINS IN EVALUATING TYPHOID VACCINES

Tully

Currie

1962

J Bacteriol

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negative strains of Salmonella typhosa: attempts to induce W-V reversion and the use of non-Vi strains in evaluating typhoid vaccines. J. Bacteriol. 84:747-753. 1962.-Repeated attempts have been made to detect reversion of several W-form Salmonella typhosa strains to Vi antigen-containing cultures. Passage of the 0-901, H-901, and Ty2W cultures through various mouse strains did not result in the recovery of V-form typhoid bacilli. Rabbits immunized repeatedly with non-Vi strains of typhoid bacilli, or with W cultures successively passed through mice, did not respond with Vi antibody formation. Attempts also were made to detect reversion of non-Vi strains passed in broth and plate cultures to which heat-killed Vi antigen-containing strains had been added. No evidence was obtained that the selection of Vi-containing cultures had been enhanced. The non-Vi typhoid strains employed in this study thus appeared to be stable W-form cultures. Viable-cell vaccines and acetone-killed and dried (AKD) vaccines of V-and W-form S. typhosa were compared in active mouse protection tests against intraperitoneal and intracerebral challenges with V-and W-form typhoid strains. Only Vi-containing cultures effectively protected mice against virulent V-form challenges, regardless of the route utilized. Greater quantities of both viable-cell and AKD vaccines prepared from non-Vi strains were required for protection against Vas well as W-form S. typhosa challenges. The observations recorded by Kauffmann (1935), besides confirming Felix and Pitt's

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%