H<sub>2</sub>O<sub>2</sub>‐induced up‐regulation of CatSper3 in mouse brain

Kim, Chang-Woon; Tak, Hyun-Min; Kim, Gyu-Tae; Mun, Yun-Ja; Jeon, Byeong Tak; Kim, Hyun Joon; Roh, Gu Seob; Han, Jaehee; Kang, Dawon

doi:10.1002/mrd.21216

Cited by 7 publications

(1 citation statement)

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“…Catsper1 mRNA expression has been detected in early stages of spermatogenesis [ 11 ]. Except for Catsper3 , which has been detected in the brain, the expression of the CatSper genes has been observed only in meiotic and post-meiotic sperm cells [ 12 – 14 ]. It has also been shown that Catsper2 is expressed before Catsper 1, 3 and 4, and this differential expression may be associated with different groups of genes expressed specifically in testis [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Regulation of CATSPER1 expression by the testis-determining gene SRY

et al. 2018

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CATSPER1 gene encodes a pore-forming and pH-sensing subunit of the CatSper Ca2+- permeable channel, a protein in the flagellum essential for sperm hyperactivation. Previous studies have shown that the murine Catsper1 gene promoter is regulated by different Sox proteins. Likewise, it is acknowledged that the human CATSPER1 gene promoter sequence is enriched in potential interaction sites for the sex-determining region Y gene (SRY), which suggest a novel regulatory transcriptional mechanism for CatSper1 channel expression. Therefore, in this work, we sought to determine whether the human CATSPER1 gene expression is regulated by the SRY transcription factor. To this end, a series of deletions and mutations were introduced in the wild- type CATSPER1 gene promoter to eliminate the SRY sites, and the different constructs were tested for their ability to activate transcription in human embryonic kidney and murine spermatogonial germ cell lines (HEK-293 and GC1-spg, respectively) using luciferase assays. In addition, by using a strategy that combines electrophoretic mobility shift assays (EMSA) and chromatin immunoprecipitation (ChIP) we investigated whether the CATSPER1 gene expression is regulated by the SRY transcription factor both in vitro and in vivo. Our results show that the transcriptional factor SRY specifically binds to different sites in the promoter sequence and has the ability to control CATSPER1 gene transcription.

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Section: Introductionmentioning

confidence: 99%

Regulation of CATSPER1 expression by the testis-determining gene SRY

et al. 2018

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Autophagy is required for proper meiosis of porcine oocytes maturing in vitro

Shen

Jin

Liang

et al. 2018

Sci Rep

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Autophagy is an essential cellular mechanism that degrades cytoplasmic proteins and organelles to recycle their components; however, the contribution of autophagy during meiosis has not been studied in porcine oocytes maturing in vitro. In this study, we observed that the autophagy-related gene, LC3, was expressed in porcine oocytes during maturation for 44 h in vitro. Knockdown of the autophagy-related gene, BECN1, reduced both BECN1 and LC3 protein expression levels. Moreover, BECN1 knockdown and treatment with the autophagy inhibitor, LY294002, during maturation of porcine oocytes in vitro impaired polar body extrusion, disturbed mitochondrial function, triggered the DNA damage response, and induced early apoptosis in porcine oocytes. Autophagy inhibition during oocyte maturation also impaired the further developmental potential of porcine oocytes. These results indicate that autophagy is required for the in vitro maturation of porcine oocytes.

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Selection of pre- versus postpubertal pig oocytes for parthenogenetic activation and somatic cell nuclear transfer

Pedersen

Liu

et al. 2015

Reprod. Fertil. Dev.

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Pig oocytes have been used increasingly for in vitro production techniques in recent years. The slaughterhouse-derived oocytes that are often used are mostly of prepubertal origin. The aims of the present study were to compare the developmental competence between pre- and postpubertal pig oocytes, and to develop a simple and practical method for the selection of prepubertal pig oocytes for parthenogenetic activation (PA) and somatic cell nuclear transfer (SCNT) based on oocyte morphology after IVM and oocyte inside zona pellucida (ZP) diameter ('small' ≤110µm; 'medium' >110µm; 'large' ≥120µm). Meiotic competence and blastocyst rates after PA and SCNT of prepubertal oocytes increased with oocyte size, with the large prepubertal oocytes reaching a level similar to postpubertal oocytes after SCNT. Blastocyst cell number was not related to oocyte inside ZP diameter and oocyte donor to the same extent as blastocyst rate. Very low blastocyst rates were obtained after PA of morphologically bad pre- and postpubertal oocytes. In conclusion, measurement of inside ZP diameter combined with morphological selection is useful to remove incompetent oocytes. Further studies are needed to clarify the relative importance of cytoplasmic volume and stage in oocyte growth phase.

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H₂O₂‐induced up‐regulation of CatSper3 in mouse brain

Cited by 7 publications

References 3 publications

Regulation of CATSPER1 expression by the testis-determining gene SRY

Regulation of CATSPER1 expression by the testis-determining gene SRY

Autophagy is required for proper meiosis of porcine oocytes maturing in vitro

Selection of pre- versus postpubertal pig oocytes for parthenogenetic activation and somatic cell nuclear transfer

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H2O2‐induced up‐regulation of CatSper3 in mouse brain

Cited by 7 publications

References 3 publications

Regulation of CATSPER1 expression by the testis-determining gene SRY

Regulation of CATSPER1 expression by the testis-determining gene SRY

Autophagy is required for proper meiosis of porcine oocytes maturing in vitro

Selection of pre- versus postpubertal pig oocytes for parthenogenetic activation and somatic cell nuclear transfer

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H₂O₂‐induced up‐regulation of CatSper3 in mouse brain