H+/Ca2+ exchange driven by the plasma membrane Ca2+‐ATPase of Arabidopsis thaliana reconstituted in proteoliposomes after calmodulin‐affinity purification

Luoni, Laura; Bonza, Maria Cristina; Michelis, Maria Ida De

doi:10.1016/s0014-5793(00)02065-2

Cited by 26 publications

(20 citation statements)

References 42 publications

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“…Calcium extrusion rates are clearly linked to Ca 2? -ATPase activity (Luoni et al 2000;Rasi-Caldogno et al 1987), and these rates play a key role in adjusting intracellular calcium concentrations (Kordyum 2003). A lower influx of calcium into cells would be expected to induce a lower efflux, a pattern seen here in the 97% decrease in the overall differential induced by nifedipine.…”

Section: Fig 4 Representative Plots Of Two Different Cells (Top and mentioning

confidence: 67%

“…-ATPases, should also allow us to further refine our model. Because calcium signaling is a universal regulatory feature of cell polarization in both plants and animals (Pickard and Fujiki 2005;Rasi-Caldogno et al 1987) the CEL-C device, which is adaptable to many cell types, could be used on future C-9 flights to test other cells which respond dramatically to the micro-g environment (Kordyum 2003;Luoni et al 2000). Such tests would reveal whether the transport phenomena reported here are evolutionarily conserved features of cellular responses to gravity or is unique to only some cell types.…”

Section: Fig 4 Representative Plots Of Two Different Cells (Top and mentioning

confidence: 99%

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Changes in gravity rapidly alter the magnitude and direction of a cellular calcium current

Salmi

Haque

Bushart

et al. 2011

Planta

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In single-celled spores of the fern Ceratopteris richardii, gravity directs polarity of development and induces a directional, trans-cellular calcium (Ca(2+)) current. To clarify how gravity polarizes this electrophysiological process, we measured the kinetics of the cellular response to changes in the gravity vector, which we initially estimated using the self-referencing calcium microsensor. In order to generate more precise and detailed data, we developed a silicon microfabricated sensor array which facilitated a lab-on-a-chip approach to simultaneously measure calcium currents from multiple cells in real time. These experiments revealed that the direction of the gravity-dependent polar calcium current is reversed in less than 25 s when the cells are inverted, and that changes in the magnitude of the calcium current parallel rapidly changing g-forces during parabolic flight on the NASA C-9 aircraft. The data also revealed a hysteresis in the response of cells in the transition from 2g to micro-g in comparison to cells in the micro-g to 2-g transition, a result consistent with a role for mechanosensitive ion channels in the gravity response. The calcium current is suppressed by either nifedipine (calcium-channel blocker) or eosin yellow (plasma membrane calcium pump inhibitor). Nifedipine disrupts gravity-directed cell polarity, but not spore germination. These results indicate that gravity perception in single plant cells may be mediated by mechanosensitive calcium channels, an idea consistent with some previously proposed models of plant gravity perception.

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Section: Fig 4 Representative Plots Of Two Different Cells (Top and mentioning

confidence: 67%

Section: Fig 4 Representative Plots Of Two Different Cells (Top and mentioning

confidence: 99%

Changes in gravity rapidly alter the magnitude and direction of a cellular calcium current

Salmi

Haque

Bushart

et al. 2011

Planta

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“…To determine if PtaGTL1 interacts with CaM in vitro , purified PtaGTL1-C (Figure 5B) was incubated with CaM-agarose from bovine testes with or without Ca 2+ . Mammalian CaM has been successfully used for identifying plant CaM-binding proteins [58]–[60]. A protein band was detected in a fraction eluted from CaM-agarose incubated with PtaGTL1-C and 2 mM CaCl 2 (Figure 5B; lane 5).…”

Section: Resultsmentioning

confidence: 99%

Poplar GTL1 Is a Ca2+/Calmodulin-Binding Transcription Factor that Functions in Plant Water Use Efficiency and Drought Tolerance

Weng¹,

Yoo²,

Gosney³

et al. 2012

PLoS ONE

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Diminishing global fresh water availability has focused research to elucidate mechanisms of water use in poplar, an economically important species. A GT-2 family trihelix transcription factor that is a determinant of water use efficiency (WUE), PtaGTL1 (GT-2 like 1), was identified in Populus tremula × P. alba (clone 717-IB4). Like other GT-2 family members, PtaGTL1 contains both N- and C-terminal trihelix DNA binding domains. PtaGTL1 expression, driven by the Arabidopsis thaliana AtGTL1 promoter, suppressed the higher WUE and drought tolerance phenotypes of an Arabidopsis GTL1 loss-of-function mutation (gtl1-4). Genetic suppression of gtl1-4 was associated with increased stomatal density due to repression of Arabidopsis STOMATAL DENSITY AND DISTRIBUTION1 (AtSDD1), a negative regulator of stomatal development. Electrophoretic mobility shift assays (EMSA) indicated that a PtaGTL1 C-terminal DNA trihelix binding fragment (PtaGTL1-C) interacted with an AtSDD1 promoter fragment containing the GT3 box (GGTAAA), and this GT3 box was necessary for binding. PtaGTL1-C also interacted with a PtaSDD1 promoter fragment via the GT2 box (GGTAAT). PtaSDD1 encodes a protein with 60% primary sequence identity with AtSDD1. In vitro molecular interaction assays were used to determine that Ca2+-loaded calmodulin (CaM) binds to PtaGTL1-C, which was predicted to have a CaM-interaction domain in the first helix of the C-terminal trihelix DNA binding domain. These results indicate that, in Arabidopsis and poplar, GTL1 and SDD1 are fundamental components of stomatal lineage. In addition, PtaGTL1 is a Ca2+-CaM binding protein, which infers a mechanism by which environmental stimuli can induce Ca2+ signatures that would modulate stomatal development and regulate plant water use.

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“…Early studies demonstrated that the inhibition of Ca 2+ pumps using vanadate and compound 48/80 induces the increase of intracellular Ca 2+ (Obermeyer and Weisenseel, 1991). Pharmacological studies on a Ca 2+ -ATPase reconstituted in proteoliposomes showed that it can act as a bona fide Ca 2+ /H + exchanger (Luoni et al, 2000). The fact that in PTs there is a high tip Ca 2+ concentration and a low pH also could be dependent, at least in part, on the activity of this pump.…”

Section: The Shank Of the Pt: Just Supporting And Buffering?mentioning

confidence: 99%

Signaling with Ions: The Keystone for Apical Cell Growth and Morphogenesis in Pollen Tubes

et al. 2016

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H⁺/Ca²⁺ exchange driven by the plasma membrane Ca²⁺‐ATPase of Arabidopsis thaliana reconstituted in proteoliposomes after calmodulin‐affinity purification

Cited by 26 publications

References 42 publications

Changes in gravity rapidly alter the magnitude and direction of a cellular calcium current

Changes in gravity rapidly alter the magnitude and direction of a cellular calcium current

Poplar GTL1 Is a Ca2+/Calmodulin-Binding Transcription Factor that Functions in Plant Water Use Efficiency and Drought Tolerance

Signaling with Ions: The Keystone for Apical Cell Growth and Morphogenesis in Pollen Tubes

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