H2 photoproduction by batch culture ofAnabaena variabilis ATCC 29413 and its mutant PK84 in a photobioreactor

Tsygankov, Anatoly A.; Borodin, V. B.; Rao, K. K.; Hall, D.O.

doi:10.1002/(sici)1097-0290(19990920)64:6<709::aid-bit10>3.0.co;2-c

Cited by 55 publications

(39 citation statements)

References 26 publications

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“…Since the hydrogen production is the result of hydrogen evolution catalyzed by nitrogenase and a hydrogen consumption catalyzed mainly by an uptake hydrogenase, the obvious improvements are to increase the hydrogen production by using alternative nitrogenases and by inhibiting the activity of the uptake hydrogenase. In a recent study, the hydrogen production by autotrophic, vanadium-grown cells (i.e., cells expressing the alternative vanadium-containing nitrogenase) of A. variabilis ATCC 29413 (wild type) and of its mutant PK84, impaired in the utilization of molecular hydrogen, was studied in a photobioreactor (204). The highest hydrogen production rates were observed in cultures grown at gradually increased irradiation.…”

Section: Rates Of Cyanobacterial Hydrogen Productionmentioning

confidence: 99%

“…The wild-type strain evolved hydrogen only under a argon atmosphere, with the actual rate being as high as the potential rate (61% of oxygenic photosynthesis used for hydrogen production). In contrast, PK84 cells also produced hydrogen during growth under carbon dioxide-enriched air (13% of oxygenic photosynthesis used for hydrogen production, representing 33% of the potential rate) (204). A. variabilis PK84 has also been examined under simulated outdoor conditions (31).…”

Section: Rates Of Cyanobacterial Hydrogen Productionmentioning

confidence: 99%

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Hydrogenases and Hydrogen Metabolism of Cyanobacteria

Tamagnini

Axelsson

Lindberg

et al. 2002

Microbiol Mol Biol Rev

466

372

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Cyanobacteria may possess several enzymes that are directly involved in dihydrogen metabolism: nitrogenase(s) catalyzing the production of hydrogen concomitantly with the reduction of dinitrogen to ammonia, an uptake hydrogenase (encoded by hupSL) catalyzing the consumption of hydrogen produced by the nitrogenase, and a bidirectional hydrogenase (encoded by hoxFUYH) which has the capacity to both take up and produce hydrogen. This review summarizes our knowledge about cyanobacterial hydrogenases, focusing on recent progress since the first molecular information was published in 1995. It presents the molecular knowledge about cyanobacterial hupSL and hoxFUYH, their corresponding gene products, and their accessory genes before finishing with an applied aspect—the use of cyanobacteria in a biological, renewable production of the future energy carrier molecular hydrogen. In addition to scientific publications, information from three cyanobacterial genomes, the unicellular Synechocystis strain PCC 6803 and the filamentous heterocystous Anabaena strain PCC 7120 and Nostoc punctiforme (PCC 73102/ATCC 29133) is included

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Section: Rates Of Cyanobacterial Hydrogen Productionmentioning

confidence: 99%

Section: Rates Of Cyanobacterial Hydrogen Productionmentioning

confidence: 99%

Hydrogenases and Hydrogen Metabolism of Cyanobacteria

Tamagnini

Axelsson

Lindberg

et al. 2002

Microbiol Mol Biol Rev

466

372

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“…Among other benefits, the genome sequence will facilitate location of mutation sites in the ⌽(PnirA-mutS) transformants evolved under specific conditions, using techniques such as genomic mapping by functional complementation (14). The biotechnological uses of cyanobacteria are numerous and wide ranging and include directed mutation of genes related to photosynthesis, bioremediation (28), and industrial hydrogen production (37). Systems such as ⌽(PnirA-mutS) could play a key role in adapting cyanobacteria to perform optimally in these tasks.…”

Section: Vol 69 2003 Cyanobacterial Hypermutator Strain 6431mentioning

confidence: 99%

Nitrogen-Regulated Hypermutator Strain of Synechococcus sp. for Use in In Vivo Artificial Evolution

Emlyn-Jones

Price

Andrews

2003

Appl Environ Microbiol

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Artificially evolved variants of proteins with roles in photosynthesis may be selected most conveniently by using a photosynthetic organism, such as a cyanobacterium, whose growth depends on the function of the target protein. However, the limited transformation efficiency of even the most transformable cyanobacteria wastes much of the diversity of mutant libraries of genes produced in vitro, impairing the coverage of sequence space. This highlights the advantages of an in vivo approach for generating diversity in the selection organism itself. We constructed two different hypermutator strains of Synechococcus sp. strain PCC 7942 by insertionally inactivating or nutritionally repressing the DNA mismatch repair gene, mutS. Inactivation of mutS greatly increases the mutation rate of the cyanobacterium's genes, leading to an up-to-300-fold increase in the frequency of resistance to the antibiotics rifampin and spectinomycin. In order to control the rate of mutation and to limit cellular damage resulting from prolonged hypermutation, we placed the uninterrupted mutS gene in the cyanobacterial chromosome under the transcriptional control of the cyanobacterial nirA promoter, which is repressed in the presence of NH 4 ؉ as an N source and derepressed in its absence. By removing or adding this substrate, hypermutation was activated or repressed as required. As expected, hypermutation caused by repression in PnirA-mutS transformants led to an accumulation of spectinomycin resistance mutations during growth.Artificial evolution (sometimes called directed or laboratory evolution) has, in recent years, been applied to a wide range of individual genes, successfully changing properties of encoded proteins ranging from thermotolerance, to catalytic specificity, to total activity (reviewed in references 3, 21, 29, 35, and 39). Using artificial evolution, whole organisms also can be evolved rapidly to exhibit new and complex traits (25,40).Techniques for artificial evolution typically entail a mutagenesis step, in which sequence diversity is generated in particular genes, and a step in which phenotypic expression of variant genes confers the desired characteristic that is selected. These techniques range from entirely in vitro procedures (for example, in vitro co-compartmentalization of an evolved gene and its protein product in aqueous droplets emulsified in oil [10]) to fully in vivo procedures. One of the major factors that limit the efficacy of artificial evolution is the number of permutations of a particular sequence that can be screened for the desired functional characteristic (sequence-space coverage). In many artificial evolution protocols, mutagenesis is performed in vitro and selection is performed in vivo. When the selection must be done in a photosynthetic organism, such as a cyanobacterium, to select variants of proteins with roles in photosynthesis, for instance, transformation efficiencies are typically low (8), and this wastes much of the generated sequence diversity. This loss can be avoided by generating the ...

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“…From mass conservation,Q out is equal to the flow rate of Argon set at 45±1 mL/min. In order to make the comparison easier with the results reported in the literature [14][15][16]22], the unit of π H 2 is converted from kg H 2 /kg dry cell/s to L/kg dry cell/h assuming that 1 kg of H 2 occupies 12.43×10 3 L at 1 atmosphere and 30 o C.…”

Section: Specific Hydrogen Production Ratementioning

confidence: 99%

Effect of nutrient media on photobiological hydrogen production by Anabaena variabilis ATCC 29413

Berberoğlu

Jay

Pilon

2008

International Journal of Hydrogen Energy

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This study reports a factor 5.5 increase in hydrogen production by Anabaena variabilis ATCC 29413 using Allen-Arnon medium compared with tobioreactor is sparged with pure argon. The parameters continuously monitored were (1) the cyanobacteria concentration, (2) the pH, (3) the dissolved oxygen concentration, (4) the nitrate and (5) the ammonia concentrations in the medium, and (6) the hydrogen concentration in the effluent gas. The three media BG-11, BG-11 o , and Allen-Arnon were tested under otherwise similar conditions. The cyanobacteria concentrations during Stage 2 were 1.10 and 1.17 kg dry cell/m 3 with BG-11 and Allen-Arnon media, respectively, while it could not exceed 0.76 kg dry cell/m 3 with medium BG-11 o . Moreover, the heterocyst frequency was 5%, 4%, and 9% for A.variabilis grown in BG-11, BG-11 o , and Allen-Arnon media. The average specific hydrogen production rates were about 8.0×10 −5 and 7.2×10 −5 kg H 2 /kg dry cell/h (1 and 0.9 L H 2 /kg dry cell/h at 1 atmosphere and 30 o C) in media BG-11 and BG-11 o , respectively. In contrast, it was about 4.5×10 −4 kg H 2 /kg dry cell/h (5.6 L H 2 /kg dry cell/h at 1 atmosphere and 30 o C) in Allen-Arnon medium.The maximum light to hydrogen energy conversion efficiencies achieved were 0.26%, 0.16%, and 1.32% for BG-11, BG-11 o , and Allen-Arnon media, respectively. The larger heterocyst frequency, specific hydrogen production rates, efficiencies, and cyanobacteria concentrations achieved using Allen-Arnon medium were attributed to higher concentrations of magnesium, calcium, sodium, and potassium in the medium. Finally, presence of vanadium in Allen-Arnon medium could have induced the transcription of vanadium based nitrogenase which is capable of evolving more hydrogen than molybdenum based one.

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H2 photoproduction by batch culture ofAnabaena variabilis ATCC 29413 and its mutant PK84 in a photobioreactor

Cited by 55 publications

References 26 publications

Hydrogenases and Hydrogen Metabolism of Cyanobacteria

Hydrogenases and Hydrogen Metabolism of Cyanobacteria

Nitrogen-Regulated Hypermutator Strain of Synechococcus sp. for Use in In Vivo Artificial Evolution

Effect of nutrient media on photobiological hydrogen production by Anabaena variabilis ATCC 29413

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