H2O2 Is Involved in the Metallothionein-Mediated Rice Tolerance to Copper and Cadmium Toxicity

Zhang, Hongxiao; Lv, Shufang; Xu, Huawei; Hou, Dan; Li, Youjun; Wang, Fayuan

doi:10.3390/ijms18102083

Cited by 46 publications

(11 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Transgenic plants accumulate more Cd 2+ in root when compared to the wild type, suggest that ZjMT may have a function in Cd 2+ retention in roots and, therefore, decrease the toxicity of Cd 2+ . Consistent with our results, heterologous expression of ricMT in transgenic rice (Zhang et al, 2017) and OsMT2c in transgenic Arabidopsis (Liu et al, 2015) provided increased tolerance against Cd stress.…”

Section: Discussionsupporting

confidence: 91%

Accumulation of cadmium (Cd) in T1 transgenic tobacco seedlings expressing metallothionein gene from Eleusine indica

et al. 2019

View full text Add to dashboard Cite

Cadmium (Cd) contamination of urban and agricultural soils is toxic to humans, animals and may cause negative effects on plant growth and crop production. The existing conventional methods are found to be not efficient to remove Cd from contaminated soil. The present experiment reports the analysis of nine T1 lines of transgenic tobacco carrying metallothionein gene (eiMT1) from Eleusine indica, with potential for high efficiency to remediate Cd in contaminated soils. Thirty-days old tobacco plants were treated with different concentrations of CdNO3 (0, 50, 100 and, 150 µmol) for seven days and the accumulation of Cd in the whole seedling was quantitatively determined by using atomic absorption spectrometer (AAS). All transgenic tobacco lines showed greater tolerance and accumulated higher level of Cd than the wild type with lines 18D, 20D1 and, 18C were among the highest (678.7, 623.0 and 611.9 mgkg-1 Cd, respectively). Meanwhile, transgenic tobacco lines 18B1 and 20D1 showed higher expression of eiMT1 gene. These results suggest that the cadmium accumulation in transgenic tobacco did not strictly associate with the expression level of eiMT1 gene. However, expression of eiMT1 greatly required for higher accumulation of Cd in transgenic tobacco seedling.

show abstract

Section: Discussionsupporting

confidence: 91%

Accumulation of cadmium (Cd) in T1 transgenic tobacco seedlings expressing metallothionein gene from Eleusine indica

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Functional yeast analysis of the cDNA library was undertaken, which resulted in the identification of several genes (including the date palm PdMT2A gene) that could play a critical role in salinity tolerance in date palm [18]. MTs are well known for detoxification of heavy metal homeostasis [28,42,43]. However, MTs have also been shown to play a role in drought and salinity tolerance [33,44].…”

Section: Discussionmentioning

confidence: 99%

Overexpression of a Metallothionein 2A Gene from Date Palm Confers Abiotic Stress Tolerance to Yeast and Arabidopsis thaliana

Patankar

Al-Harrasi

Kharusi

et al. 2019

IJMS

View full text Add to dashboard Cite

Although the date palm tree is an extremophile with tolerance to drought and certain levels of salinity, the damage caused by extreme salt concentrations in the soil, has created a need to explore stress-responsive traits and decode their mechanisms. Metallothioneins (MTs) are low-molecular-weight cysteine-rich proteins that are known to play a role in decreasing oxidative damage during abiotic stress conditions. Our previous study identified date palm metallothionein 2A (PdMT2A) as a salt-responsive gene, which has been functionally characterized in yeast and Arabidopsis in this study. The recombinant PdMT2A protein produced in Escherichia coli showed high reactivity against the substrate 5′-dithiobis-2-nitrobenzoic acid (DTNB), implying that the protein has the property of scavenging reactive oxygen species (ROS). Heterologous overexpression of PdMT2A in yeast (Saccharomyces cerevisiae) conferred tolerance to drought, salinity and oxidative stresses. The PdMT2A gene was also overexpressed in Arabidopsis, to assess its stress protective function in planta. Compared to the wild-type control, the transgenic plants accumulated less Na+ and maintained a high K+/Na+ ratio, which could be attributed to the regulatory role of the transgene on transporters such as HKT, as demonstrated by qPCR assay. In addition, transgenic lines exhibited higher chlorophyll content, higher superoxide dismutase (SOD) activity and improved scavenging ability for reactive oxygen species (ROS), coupled with a better survival rate during salt stress conditions. Similarly, the transgenic plants also displayed better drought and oxidative stress tolerance. Collectively, both in vitro and in planta studies revealed a role for PdMT2A in salt, drought, and oxidative stress tolerance.

show abstract

“…CUP1 in S. cerevisiae and LbMT1 and LbMT2 from Laccaria bicolor were all identified as having a key role in maintaining the local redox balance by preventing the Fenton reaction and scavenging ROS. Zhang et al found that the overexpression of ricMT blocked H 2 O 2 accumulation and significantly increased the survival rate of rice cells stressed by Cu and Cd. DPPH free radicals were found to be cleared by yeast MTs in vitro in the concentration range of 20–100 μg·mL −1 .…”

Section: Discussionmentioning

confidence: 99%

Cloning and functional characterization of a novel metallothionein gene in Antarctic sea‐ice yeast (Rhodotorula mucilaginosa)

Kan

Zhou

et al. 2019

J Basic Microbiol

View full text Add to dashboard Cite

Metallothionein (MT) is a low-molecular-weight protein with a high metal binding capacity and plays a key role in organism adaptation to heavy metals. In this study, a metallothionein gene was successfully cloned and sequenced from Antarctic sea-ice yeast Rhodotorula mucilaginosa AN5. Nucleotide sequencing and analysis revealed that the gene had four exons interrupted by three introns. MTs complementary DNA (named as RmMT) had an open reading frame of 321 bp encoding a 106 amino acid protein with a predicted molecular weight of 10.3 kDa and pI of 8.49. The number of amino acids and distribution of cysteine residues indicated that RmMT was a novel family of fungal MTs. Quantitative real-time polymerase chain reaction analysis showed that RmMT expression was elevated under copper-induced stress. TheRmMT gene was transferred into E. coli and the RmMT expressing bacteria showed improved tolerance to copper ion and increased accumulation of heavy metals, such as Cu 2+ , Pb 2+ , Zn 2+ , Cd 2+ , and Ag + . Moreover, in vitro studies, purified recombinant RmMT demonstrated that it could be used as a good scavenger of superoxide anion, hydroxyl, and 1,1-Diphenyl-2-picrylhydrazyl (DPPH) radicals. In summary, these results demonstrate that RmMT plays a key role in the tolerance and bioaccumulation of heavy metals. K E Y W O R D Sfunctional analysis, heavy metal, metallothionein, polar yeast

show abstract

H2O2 Is Involved in the Metallothionein-Mediated Rice Tolerance to Copper and Cadmium Toxicity

Cited by 46 publications

References 46 publications

Accumulation of cadmium (Cd) in T1 transgenic tobacco seedlings expressing metallothionein gene from Eleusine indica

Accumulation of cadmium (Cd) in T1 transgenic tobacco seedlings expressing metallothionein gene from Eleusine indica

Overexpression of a Metallothionein 2A Gene from Date Palm Confers Abiotic Stress Tolerance to Yeast and Arabidopsis thaliana

Cloning and functional characterization of a novel metallothionein gene in Antarctic sea‐ice yeast (Rhodotorula mucilaginosa)

Contact Info

Product

Resources

About