H2S Regulation of Metabolism in Cucumber in Response to Salt-Stress Through Transcriptome and Proteome Analysis

Jiang, Jianhui; Ren, Xuming; Li, Li; Hou, Ruping; Sun, Wen‐Yih; Jiao, Chengjin; Yang, Ni; Dong, Yanxin

doi:10.3389/fpls.2020.01283

Cited by 50 publications

(24 citation statements)

References 52 publications

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“…DEGs identified in the DND252 variety were primarily enriched in cellular amide metabolic processes, cell, binding, and catalytic activity, whereas DEGs identified in the HN84 variety were primarily enriched in cellular processes, membranes, and binding. A transcriptome analysis of cucumber treated with H 2 S showed that the DEGs were primarily enriched in inherent components of the membrane [31], which is similar to the findings of the present study, suggesting that sulfur affects the cell membranes.…”

Section: Discussionsupporting

confidence: 89%

Transcriptome Analysis of Soybean in Response to Different Sulfur Concentrations

Wang¹,

Li²,

Wu³

et al. 2022

Phyton

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Sulfur is an indispensable nutrient for plant growth and development, and is important in the synthesis of sulfurcontaining amino acids. Although several studies on the effects of some macronutrients, including nitrogen and phosphorus, have been conducted on the performance of several crops at the genomic level, studies on the effect of sulfur on crop performance are limited. Therefore, this study aimed to examine the effect of different sulfur concentration on the transcriptome of soybean. Additionally, soybean yield parameters were also examined. Two soybean varieties, DND252 and HN84, were exposed to low and high concentrations of sulfur, and differentially expressed genes (DEGs) were identified using transcriptome analysis. The study results showed that the DEGs identified in the DND252 variety were involved in stimuli response, DNA binding and cell periphery under low sulfur concentrations. Also, the DEGs identified under high sulfur concentration were involved in membrane and membrane parts. Additionally, DEGs identified in the HN84 variety under low sulfur concentrations had similar functions as those identified in DND252 under high sulfur concentrations, indicating that HN84 was more sensitive to sulfur concentration changes than DND252. However, under higher sulfur concentrations, the DEGs identified in HN84 were primarily involved in membrane and membrane parts, indicating that high sulfur can cause cell membrane damage. Furthermore, soybean grown using 2.0 mmol/L sulfur had the best yield. The findings of this study identified candidate genes for the breeding and development of sulfur-efficient soybean varieties.

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Section: Discussionsupporting

confidence: 89%

Transcriptome Analysis of Soybean in Response to Different Sulfur Concentrations

Wang¹,

Li²,

Wu³

et al. 2022

Phyton

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“…H 2 S could promote photosynthetic electron transfer, chlorophyll biosynthesis and carbon fixation in Kandelia obovata leaves and cucumber under salt stress (Jiang et al 2020 ; Liu et al 2020d ). In addition, the abundance of other proteins related to the metabolic pathways, such as antioxidation (APX, copper/zinc superoxide dismutase, pancreatic and duodenal homeobox 1), protein synthesis (heat-shock protein (HSP), chaperonin family protein 20 and Cysteine synthase 1), nitrogen metabolism (glutamine synthetase 1 and 2), glycolysis (phosphoglycerate kinase and triosephosphate isomerase), and the AsA-GSH cycle (glutathione S-transferase U25-like), was increased by H 2 S under high salinity (Jiang et al 2020 ; Liu et al 2020d ). However, the mechanism underlying the effect of H 2 S on such huge proteins remains unclear (Guo et al 2018 ; Li et al 2014a , 2020c ).…”

Section: H 2 S Positively Responds To Biotic and Amentioning

confidence: 99%

Hydrogen sulfide (H2S) signaling in plant development and stress responses

et al. 2021

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Hydrogen sulfide (H 2 S) was initially recognized as a toxic gas and its biological functions in mammalian cells have been gradually discovered during the past decades. In the latest decade, numerous studies have revealed that H 2 S has versatile functions in plants as well. In this review, we summarize H 2 S-mediated sulfur metabolic pathways, as well as the progress in the recognition of its biological functions in plant growth and development, particularly its physiological functions in biotic and abiotic stress responses. Besides direct chemical reactions, nitric oxide (NO) and hydrogen peroxide (H 2 O 2 ) have complex relationships with H 2 S in plant signaling, both of which mediate protein post-translational modification (PTM) to attack the cysteine residues. We also discuss recent progress in the research on the three types of PTMs and their biological functions in plants. Finally, we propose the relevant issues that need to be addressed in the future research. Graphic abstract Supplementary Information The online version contains supplementary material available at 10.1007/s42994-021-00035-4.

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“…Furthermore, elevated invertase activity ( Figure 9 E) and higher accumulation of TSS ( Figure 10 C) indicate enhanced sucrose metabolism, thus leading to the formation of hexose sugars during K + supplementation under NaCl stress. Although not much information is available on the role of H 2 S in the modulation of sugar metabolism in plants, Jiang et al [ 53 ] provide a proteomic analysis of H 2 S-responsive proteins, wherein various enzymes of carbohydrate metabolism are modulated in NaCl-stressed cucumber leaves. Modulation of α-amylase and β-amylase activity ( Figure 9 A,B) during NaCl stress and K + and HT treatment indicates the regulation of starch degradation, mediated by endogenous H 2 S signaling.…”

Section: Discussionmentioning

confidence: 99%

Exogenous Potassium (K+) Positively Regulates Na+/H+ Antiport System, Carbohydrate Metabolism, and Ascorbate–Glutathione Cycle in H2S-Dependent Manner in NaCl-Stressed Tomato Seedling Roots

Khan

Bhatla

Al-Huqail

et al. 2021

Plants

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Potassium (K+) is one of the vital macronutrients required by plants for proper growth and blossoming harvest. In addition, K+ also plays a decisive role in promoting tolerance to various stresses. Under stressful conditions, plants deploy their defense system through various signaling molecules, including hydrogen sulfide (H2S). The present investigation was carried out to unravel the role of K+ and H2S in plants under NaCl stress. The results of the study show that NaCl stress caused a reduction in K+ and an increase in Na+ content in the tomato seedling roots which coincided with a lower H+-ATPase activity and K+/Na+ ratio. However, application of 5 mM K+, in association with endogenous H2S, positively regulated the Na+/H+ antiport system that accelerated K+ influx and Na+ efflux, resulting in the maintenance of a higher K+/Na+ ratio. The role of K+ and H2S in the regulation of the Na+/H+ antiport system was validated by applying sodium orthovanadate (plasma membrane H+-ATPase inhibitor), tetraethylammonium chloride (K+ channel blocker), amiloride (Na+/H+ antiporter inhibitor), and hypotaurine (HT, H2S scavenger). Application of 5 mM K+ positively regulated the ascorbate–glutathione cycle and activity of antioxidant enzymes that resulted in a reduction in reactive oxygen species generation and associated damage. Under NaCl stress, K+ also activated carbohydrate metabolism and proline accumulation that caused improvement in osmotic tolerance and enhanced the hydration level of the stressed seedlings. However, inclusion of the H2S scavenger HT reversed the effect of K+, suggesting H2S-dependent functioning of K+ under NaCl stress. Therefore, the present findings report that K+, in association with H2S, alleviates NaCl-induced impairments by regulating the Na+/H+ antiport system, carbohydrate metabolism, and antioxidative defense system.

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H2S Regulation of Metabolism in Cucumber in Response to Salt-Stress Through Transcriptome and Proteome Analysis

Cited by 50 publications

References 52 publications

Transcriptome Analysis of Soybean in Response to Different Sulfur Concentrations

Transcriptome Analysis of Soybean in Response to Different Sulfur Concentrations

Hydrogen sulfide (H2S) signaling in plant development and stress responses

Exogenous Potassium (K+) Positively Regulates Na+/H+ Antiport System, Carbohydrate Metabolism, and Ascorbate–Glutathione Cycle in H2S-Dependent Manner in NaCl-Stressed Tomato Seedling Roots

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