HA-g-CS Implant and Moderate-intensity Exercise Stimulate Subchondral Bone Remodeling and Promote Repair of Osteochondral Defects in Mice

Ke, Shen; Liu, Xiaonan; Qin, Hanjun; Chai, Yifeng; Wang, Lei; Yu, Bin

doi:10.7150/ijms.63401

Cited by 8 publications

(4 citation statements)

References 62 publications

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“…Lu et al have demonstrated that the chitosan solution injected into the knee articular cavity of rats caused a significant increase in the density of chondrocyte in the knee articular cartilage, suggesting that the chitosan could be potentially beneficial to the wound healing of articular cartilage. In addition, a study by Shen et al found that hydroxyapatite-grafted-chitosan promoted subchondral bone remodeling in arthritic mice and promoted osteogenesis and chondrogenesis [ 20 ]. This implies that chitosan can be used as a material for subchondral bone repair and has a synergistic effect on cartilage repair.…”

Section: Discussionmentioning

confidence: 99%

“…Chitosan is a deacetylated derivative from chitin with good biocompatibility and is widely used in therapies including wound healing, vascular tissue engineering and dental implants [ 17 – 19 ]. In addition, one study reported that hydroxyapatite-grafted-chitosan promoted subchondral bone remodeling, osteogenesis and chondrogenesis [ 20 ]. It is promising in the treatment of osteoarthritis repair as a base for osteoplastic materials with its good biocompatibility [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

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Icariin-conditioned serum combined with chitosan attenuates cartilage injury in rabbit knees with osteochondral defect

et al. 2023

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Background Knee osteoarthritis (KOA) is one of the most common degenerative diseases. Its development is closely related to cartilage injury and subchondral bone remodeling homeostasis. In the present study, we combined icariin-conditioned serum (ICS) with thiolated chitosan (CSSH), a material widely used in tissue engineering for cartilage repair, to demonstrate its effect on the repair of cartilage damage and abnormal subchondral remodeling. Methods New Zealand rabbits were undergoing surgery for cartilage defect, and joint cavity injection was performed in each group with 0.5 mL normal saline (NS), ICS, CSSH and ICS-CSSH in the right joint every week for five times. Positioning performance was observed using VICON motion capture system. Glycosaminoglycans (GAG) secretion of articular fluid was assessed. Osteoarthritis Research Society International (OARSI) score and immunohistochemical (IHC) analysis including H&E, Safranin O and collagen II staining were employed to evaluate the morphologic repair of cartilage and subchondral bone. mRNA expression of COL2A1, MMP13 and ADAMTS5 was detected in chondrocytes from injury area. Results ICS combined with CSSH attenuated cartilage injury and abnormal subchondral remodeling in rabbits with KOA. ICS and CSSH groups showed slight improvement in positioning performance, while ICS-CSSH group exhibited better positioning performance. ICS-CSSH group showed increased GAG secretion of articular fluid and expression of COL2A1 in articular chondrocytes. Furthermore, both macroscopic observation and IHC analysis showed femoral condyle in ICS-CSSH rabbits were repaired with more native cartilage and subchondral bone regeneration. Conclusions ICS combined with CSSH could promote the repair of osteochondral defect and stabilize subchondral bone remodeling in rabbit knees.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Icariin-conditioned serum combined with chitosan attenuates cartilage injury in rabbit knees with osteochondral defect

et al. 2023

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“…The bone formation promoted by HC + ME is accompanied by the increase of type H blood vessels, osteoclasts and osteoblast lineage, indicating that bone remodeling is active. This therapeutic approach upregulates the expression level of IGF‐1, BMP‐2 in subchondral bone, which induces the high expression of SOX‐9 83 …”

Section: Tissue Engineering Strategies For Promoting Bone Regeneratio...mentioning

confidence: 99%

Type H blood vessels in coupling angiogenesis‐osteogenesis and its application in bone tissue engineering

Kusumbe

Cai

et al. 2023

J Biomed Mater Res

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One specific capillary subtype, termed type H vessel, has been found with unique functional characteristics in coupling angiogenesis with osteogenesis. Researchers have fabricated a variety of tissue engineering scaffolds to enhance bone healing and regeneration through the accumulation of type H vessels. However, only a limited number of reviews discussed the tissue engineering strategies for type H vessel regulation. The object of this review is to summary the current utilizes of bone tissue engineering to regulate type H vessels through various signal pathways including Notch, PDGF-BB, Slit3, HIF-1α, and VEGF signaling. Moreover, we give an insightful overview of recent research progress about the morphological, spatial and agedependent characteristics of type H blood vessels. Their unique role in tying angiogenesis and osteogenesis together via blood flow, cellular microenvironment, immune system and nervous system are also summarized. This review article would provide an insight into the combination of tissue engineering scaffolds with type H vessels and identify future perspectives for vasculized tissue engineering research.

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“…In a mouse model with induced muscle injury, researchers observed how physical activity affects the production of osteogenesis and chondrogenesis factors [ 96 ]. In a previous study by Fry CS et al, satellite cells have been shown to be necessary for the maintenance of physical function and for the increase in muscle fiber size in response to physical activity throughout life.…”

Section: In Vitro and In Vivo Model To Evaluate The Effects Of Physic...mentioning

confidence: 99%

Crosstalk between Bone and Muscles during Physical Activity

Dalle Carbonare,

Minoia,

Zouari

et al. 2023

Cells

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Bone–muscle crosstalk is enabled thanks to the integration of different molecular signals, and it is essential for maintaining the homeostasis of skeletal and muscle tissue. Both the skeletal system and the muscular system perform endocrine activity by producing osteokines and myokines, respectively. These cytokines play a pivotal role in facilitating bone–muscle crosstalk. Moreover, recent studies have highlighted the role of non-coding RNAs in promoting crosstalk between bone and muscle in physiological or pathological conditions. Therefore, positive stimuli or pathologies that target one of the two systems can affect the other system as well, emphasizing the reciprocal influence of bone and muscle. Lifestyle and in particular physical activity influence both the bone and the muscular apparatus by acting on the single system but also by enhancing its crosstalk. Several studies have in fact demonstrated the modulation of circulating molecular factors during physical activity. These molecules are often produced by bone or muscle and are capable of activating signaling pathways involved in bone–muscle crosstalk but also of modulating the response of other cell types. Therefore, in this review we will discuss the effects of physical activity on bone and muscle cells, with particular reference to the biomolecular mechanisms that regulate their cellular interactions.

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HA-g-CS Implant and Moderate-intensity Exercise Stimulate Subchondral Bone Remodeling and Promote Repair of Osteochondral Defects in Mice

Cited by 8 publications

References 62 publications

Icariin-conditioned serum combined with chitosan attenuates cartilage injury in rabbit knees with osteochondral defect

Icariin-conditioned serum combined with chitosan attenuates cartilage injury in rabbit knees with osteochondral defect

Type H blood vessels in coupling angiogenesis‐osteogenesis and its application in bone tissue engineering

Crosstalk between Bone and Muscles during Physical Activity

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