Habitat phenotyping of two sub-Antarctic flies by metabolic fingerprinting: Evidence for a species outside its home?

Laparie, Mathieu; Bical, Raphael; Larvor, Vanessa; Vernon, Philippe; Frénot, Yves; Renault, David

doi:10.1016/j.cbpa.2012.04.022

Cited by 10 publications

(13 citation statements)

References 40 publications

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“…For each population, two to three individuals were pooled in each replicate (four to seven replicates per experimental condition, depending on survival and individual masses) to obtain sufficient material for the quantification of free amino acids (glycine, serine, ornithine, leucine, valine, isoleucine, proline, glutamic acid, phenylalanine, putrescine and threonine), free sugars (ribose, glucose, maltose and trehalose) and three other metabolites (ethanolamine, inositol and glycerol). These compounds were extracted from lyophilized organisms according to Laparie et al (Laparie et al, 2012). Pools of lyophilized animals were weighed and then homogenized in 300μl of a mix of methanol/chloroform (2:1) using a bead-beating device (Retsch MM301, Retsch, Haan, Germany) at 25Hz for 1.5min with two tungsten beads.…”

Section: Measurement Of Total Free Amino Acids and Sugarsmentioning

confidence: 99%

Thermal tolerance breadths among groundwater crustaceans living in a thermally constant environment

Mermillod‐Blondin

Lefour

Lalouette

et al. 2013

Journal of Experimental Biology

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SUMMARYThe climate variability hypothesis assumes that the thermal tolerance breadth of a species is primarily determined by temperature variations experienced in its environment. If so, aquatic invertebrates living in thermally buffered environments would be expected to exhibit narrow thermal tolerance breadths (stenothermy). We tested this prediction by studying the thermal physiology of three isopods (Asellidae, Proasellus) colonizing groundwater habitats characterized by an annual temperature amplitude of less than 1°C. The species responses to temperature variation were assessed in the laboratory using five physiological variables: survival, locomotor activity, aerobic respiration, immune defense and concentrations of total free amino acids and sugars. The three species exhibited contrasted thermal physiologies, although all variables were not equally informative. In accordance with the climate variability hypothesis, two species were extremely sensitive even to moderate changes in temperature (2°C) below and above their habitat temperature. In contrast, the third species exhibited a surprisingly high thermal tolerance breadth (11°C). Differences in response to temperature variation among Proasellus species indicated that their thermal physiology was not solely shaped by the current temperature seasonality in their natural habitats. More particularly, recent gene flow among populations living in thermally constant yet contrasted habitats might explain the occurrence of eurytherm species in thermally buffered environments.

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Section: Measurement Of Total Free Amino Acids and Sugarsmentioning

confidence: 99%

Thermal tolerance breadths among groundwater crustaceans living in a thermally constant environment

Mermillod‐Blondin

Lefour

Lalouette

et al. 2013

Journal of Experimental Biology

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“…The samples in ethanol were vacuum-dried (Speed Vac Concentrator, MiVac, Genevac Ltd., Ipswich, England), and we used the experimental procedure described in Laparie et al (2012b) …”

Section: ▪ Separation and Derivatizationmentioning

confidence: 99%

Physiological responses to temperature in Merizodus soledadinus (Col., Carabidae), a subpolar carabid beetle invading sub-Antarctic islands

Laparie¹,

Renault

2014

Polar Biol

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Recent human activities and rising air temperature have increased the vulnerability of sub-Antarctic islands to alien species. At the Kerguelen Islands, the predaceous ground beetle Merizodus soledadinus is the only invasive insect originating from the southern cold temperate area (native from Patagonia, Tierra del Fuego and Falkland Islands). This austral origin raises the question of the limits of its physiological tolerance and capability to withstand (i) global change and (ii) warmer climates of archipelagos where its accidental transportation could be facilitated from the Kerguelen Islands (namely Amsterdam & Saint Paul). Using GC-MS metabolomics, we compared metabotypes of adults exposed to different temperatures (0, 4, 8, 12, 16, 20° C). All individuals survived after two weeks regardless of the temperature they were exposed to. The physiological changes observed were consistent with increased metabolic rate at increased temperatures, without extreme metabotypes that are characteristic of acute stress. First cues of sub-lethal stress were observed after prolonged exposure to 20° C, a warm regime unrealistic for such duration in sub-Antarctic Islands. Overall, M. soledadinus' thermal tolerance exceeded temperatures currently experienced in nature, suggesting that climate warming may boost its invasion by eliciting its activity and broadening habitat suitability in both invaded and still pristine islands. This thermal tolerance may allow survival aboard ships and development in sub-Antarctic islands with conditions warmer than the Kerguelen Islands, such as Amsterdam & St Paul. Stringent biosecurity measures are thus needed to prevent transfer from Kerguelen to these islands. The native range of this predaceous beetle limited to the Falkland Islands and southernmost South America may be partly constrained by factors other than temperature, such as desiccation, predation or competition.

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“…Percentages of body water content ranged between 62-84% and were similar across ecophenotypes (Table 1a), but differed for the two sampling locations (Ile aux Cochons 77.4 ± 4.6%, Pointe Suzanne 71.9 ± 4.9%). Differences in body mass between populations of C. moseleyi inhabiting contrasting habitats have been previously reported (Laparie et al 2012), but seem unrelated to body water content. Mechanisms other than bulk water storage, such as metabolic water storage (Gibbs et al 1997) or behavioural avoidance (Jury et al 1994), may account for differences in the response to dehydration stress of the two ecophenotypes and require further investigation.…”

Section: Resultsmentioning

confidence: 67%

“…Although the main habitat of this species is thought to be the native Kerguelen cabbage, it inhabits environments of contrasting characteristics. Previous studies have focused on ecophysiological adaptations to salinity (Laparie et al 2012, Renault et al 2014. Flies from two ecophenotypes, seaweeds and Kerguelen cabbages, were collected from two localities at the Kerguelen Islands (49° 26' S, 70° 26' E): Pointe Suzanne and Ile aux Cochons (Fig.…”

Section: Methodsmentioning

confidence: 99%

“…Calycopteryx moseleyi is able to exploit two contrasting environments, differring in their relative humidity (RH) and salinity (Laparie et al 2012, this study): the axils of the leaves of the native Kerguelen cabbage (Pringlea antiscorbutica; ~80% RH, non-saline conditions) and decomposing seaweeds in rocky seashores (~60% RH and salinity of up to 70 parts per thousand). Populations of C. moseleyi inhabiting each of these habitats have distinct molecular and morphological adaptations, in particular with relation to saline stress, resulting in two distinct ecophenotypes (Laparie et al 2012). Meanwhile, their ability to tolerate dehydration stress and capacities of underlying physiological mechanisms remain largely unknown.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Adaptations of a native Subantarctic flightless fly to dehydration stress: more plastic than we thought? ( Short Communication)

Barrio¹,

Hik²,

Charrier³

et al. 2014

Czech Polar Rep.

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Water conservation is a critical aspect affecting the survival, distribution and abundance of terrestrial arthropods. In this study we investigate mechanisms of dehydration tolerance of the native, flightless fly, Calycopteryx moseleyi, inhabiting contrasting environments at two localities in the Kerguelen Islands. We compare the survival abilities and management of body water content of adult flies from two different ecophenotypes when exposed to conditions of low relative humidity. Our results suggest a broad plasticity in the responses of C. moseleyi to desiccation, showing distinct local adaptations to environmental conditions.

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Habitat phenotyping of two sub-Antarctic flies by metabolic fingerprinting: Evidence for a species outside its home?

Cited by 10 publications

References 40 publications

Thermal tolerance breadths among groundwater crustaceans living in a thermally constant environment

Thermal tolerance breadths among groundwater crustaceans living in a thermally constant environment

Physiological responses to temperature in Merizodus soledadinus (Col., Carabidae), a subpolar carabid beetle invading sub-Antarctic islands

Adaptations of a native Subantarctic flightless fly to dehydration stress: more plastic than we thought? ( Short Communication)

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