Habitats Ecological Characteristics of Asplenium scolopendrium L. and its RAPD Analysis

Abstract

-This study was conducted to investigate the environmental characteristics of natural habitats, and the genetic variations in samples from 19 different quadrates of Asplenium scolopendrium 10 habitats. The natural habitats of A. scolopendrium were located at an altitudes 110-973 m with inclinations of 7-30°. All the quadrates were located on north facing slopes. The average field capacity of the soil is 31.4%, with organic matter at 16.7%, and the pH is 5.84. The soil texture was confirmed as sandy loam in 8 habitats and silt loam in 2 habitats. A total of 214 vascular plants were identified from 10 habitats. The importance value of the herbaceous layer (H) was the highest in A. scolopendrium, at 10.4%, followed by Arachniodes standishii (7.3%), Dryopteris crassirhizoma (6.5%), and Polystichum tripteron (5.3%), which implies that the natural habitats of A. scolopendrium are affinity with ferns. The species diversity of A. scolopendrium was estimated as on average 1.09, while the dominance and evenness were 0.12 and 0.87, respectively. The result of the RAPD analysis, among 59 bands amplified with a primer, 25 (42.4%) showed polymorphism. Twenty-one individuals of 10 habitats could be classified into four groups with similarity coefficient values ranging from 0.74 to 1.0. Mt. Mullae and Geumdaebong populations shows basal branching within the 21 individuals. Ulleung-do island and Jeju-do island population forms an independent clade, respectively. Mt. Moak and Byeonsan-bando clade formed a sister to the Ulleung-do island and Jeju-do island clade.

Frond Optical Properties of the Fern Phyllitis scolopendrium Depend on Light Conditions in the Habitat

“…Hart’s-tongue fern, Phyllitis (syn. Asplenium ) scolopendrium (L.) Newm., is a perennial, rhizomatous, evergreen fern belonging to the Aspleniaceae family [ 32 ]. It is also a calciphile species, requiring habitats that are more or less permanently moist [ 29 ].…”

Assessment of genetic diversity and distance of three Cicuta virosa populations in South Korea

“…To study the genetic difference among three populations, we used the RAPD method that has been used for investigating the genetic diversity and structure of populations and that is independent to the former knowledge of their DNA sequence (Williams et al 1990, Newbury and Ford-Lloyd 1993, Beebee and Graham 2008. This method has been used for understanding the formation and maintenance of natural plant populations (Bonnin et al 1996, Min et al 2012, intraspecific phylogenic studies (Kim et al 2008, Ok andYoo 2012), and genetic difference among the varieties or ecotypes (Virk et al 1995, Ray and Roy 2009, Arghavani et al 2010. The aim of this study is to assess the genetic diversity within three C. virosa populations in Korea and the differences among them using RAPD markers and to understand the relationship between the genetic difference and ecotypic differences of .…”

The complete chloroplast genome sequence of Asplenium komarovii Akasawa, a rare fern in South Korea

“…japonicum (Komarov) Rasbach, Reichstein & Viane) is distributed in Korea, Japan, China, and Russia. In Korea, it is found in the inland regions of Gangwon-do and Jeolla-do, and the two islands of Ulleung and Jeju (Ok and Yoo 2012), but most populations are very small and fragmentated. Therefore, A. komarovii has been protected as a rare plant by the Korea Forest Research Institute since 1997.…”