Habituation of acoustic startle in rats—a functional ablation study

Storozeva, Zinaida I.; Pletnicov, M V

doi:10.1097/00001756-199410270-00019

Cited by 6 publications

(3 citation statements)

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“…The experimental model consisted of shortterm and long-term habituation to acoustic startle reactions with simultaneous recording of freezing behavior [25]. Animals were acclimated to the experimental chamber for 5 min in the absence of any kind of sound stimulus, 24 h before procedures.…”

Section: Methodsmentioning

confidence: 99%

The involvement of neurotrophic factors in the central mechanisms of behavior in adult animals

Шерстнев

Сторожева²,

Gruden³

et al. 2000

Neurosci Behav Physiol

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In the light of the similarity of the neurochemical mechanisms of behavior during ontogenesis and in adult organisms, studies were performed on the effects of antibodies to neurotrophic factors--S-100b protein, CSL lectin, and R1 lectin-on the acquisition, retention, and reproduction of habituated acoustic startle reactions and freezing behavior in adult rats. Antibodies against neurotrophic factors were found to have selective, dose-dependent effects on the types of behavior investigated. Antibodies to S-100b, CSL, and R1, at a dose of 5 microg, induced derangements in the processes of consolidation and/or retention of behavioral habits. At a dose of 2 microg, antibodies to S-100b selectively blocked the retention of long-term habituation of the startle reaction, while a dose of 0.5 microg had no such effect. Antibodies to neurotrophic factors had no effect on reproduction of acquired defensive habits.

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Section: Methodsmentioning

confidence: 99%

The involvement of neurotrophic factors in the central mechanisms of behavior in adult animals

Шерстнев

Сторожева²,

Gruden³

et al. 2000

Neurosci Behav Physiol

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“…This behavior model was selected because of data indicating that the mid-cerebellum is involved in supporting long-term habituation to ASR and the production of freezing behavior in adult rats [13,17,18], and because of data indicating that protein A3G7 is present in cerebellar Purkinje cells [4].…”

Section: Methodsmentioning

confidence: 99%

The selective effects of a monoclonal antibody against neural growth-related protein A3G7 on central mechanisms of several types of defensive behavior in adult rats

Шерстнев

Pletnikov²,

Сторожева³

et al. 1999

Neurosci Behav Physiol

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Studies were carried out into the effects of monoclonal antibodies to protein A3G7, which is associated with the differentiation of cerebellar and hippocampal neurons, on the development, retention, and reproduction of a habituated acoustic startle response and freezing behavior in rats. Application of monoclonal antibody (50 ng) to the vermis of the cerebellum selectively disrupted the retention of long-term habituation of the acoustic startle response, while a higher dose (5 micrograms) inhibited both retention of long-term habituation of the acoustic startle response and conditioned freezing behavior. Monoclonal antibody (10 micrograms) disrupted the development and retention of short-term and long-term extinction of the acoustic startle response, as well as conditioned freezing behavior. These data provide experimental support for the systemogenesis hypothesis of behavioral acts and for the concept that there is a single molecular basis for development and learning.

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“…Taking into account the fact that A3G7 is located in cerebellar Purkinje cells, the effect of anti-A3G7 MAB was studied on the mode of habituation to acoustic startle reaction (ASR). Previotts studies demonstrated that medial cerebellar structures (vermis and nucleus fastigii) directly participate in ASR habituation in adult rats [7,11].The aim of the present study was to investigate the effect of anti-A3G7 MAB on acquisition, consolidation, and retrieval of short-and long-term ASR habituation in adult rats under condition of MAB application to the vermis. …”

mentioning

confidence: 89%

Monoclonal antibodies to A3G7 protein associated with nervous tissue growth disturb learning and memory in adult rats

et al. 1998

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Effect of monoclonal antibodies to A3G7 protein specific for cerebellar Purkinje cells and hippocampal pyramidal neurons on learning, consolidation, and retrieval of shortand long-term habituation to acoustic startle reaction is studied in adult rats. Application of 1 mg/ml antibodies to the cerebellar vermis selectively disturbs long-term habituation, while 2 mg/ml antibodies inhibit both short-and long-term habituation. The data are analyzed with consideration of the involvement of A3G7 protein into nerve growth and neurotransmittory processes underlying learning and memory in developing and mature brain. Key Words: monoclonal antibodies; nerve growth factor; learning; memory; acoustic startle reactionThis study stemmed from the concept on the involvement of molecular factors that regulate growth and development of the nervous tissue into learning and memory in mature brain [4,9,12]. Of special interest are the factors regulating the differentiation of some neurons and associated with synapto-and dendritogenesis. These processes underlie integrative functions of the nervous system both in young and adult organism [6,131. We have previously obtained monoclonal antibodies (MAB) to neurospecific A3G7 protein localized in rat cerebellar Purkinje cells and hippocampal pyramidal neurons and involved into formation and stabilization of synaptic contacts [3]. It has been shown that anti-A3G7 MAB block induction of the long-term potentiation in hippocampal slices of adult rats and impair the active avoidance reaction in adult rats one day postinjection [1,5]. However, the role of A3G7 protein in learning and Laboratory of Functional Neurochemistry, P. K. Anokhin Institute of Normal Physiology, Russian Academy of Medical Sciences, Moscow; Instilute of Medical and Biological Cybernetics, Siberian Division of 1tlo Russian Academy of Medical Sciences, Novosibirsk memory remained beyond the scope of these pilot studies. Taking into account the fact that A3G7 is located in cerebellar Purkinje cells, the effect of anti-A3G7 MAB was studied on the mode of habituation to acoustic startle reaction (ASR). Previotts studies demonstrated that medial cerebellar structures (vermis and nucleus fastigii) directly participate in ASR habituation in adult rats [7,11].The aim of the present study was to investigate the effect of anti-A3G7 MAB on acquisition, consolidation, and retrieval of short-and long-term ASR habituation in adult rats under condition of MAB application to the vermis. MATERIALS AND METHODSExperiments were carried out on male Wistar rats weighing 250-300 g and maintained with food and water ad libitum (3 rats per cage). Experiments were performed in the light period from 11:00 to 15:00 Short-and long-term habituation to ASR was used as the behavioral model. Twenty-for hours before the experiment, the animals were adapted to experimental chamber for 5 min in the absence of stimuli.0007-4888/98/0008-08135"2_0,00 ~'~1999 Kluwer Acddeniic/Ph'nilnl lhllJli~h~'r~

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Habituation of acoustic startle in rats—a functional ablation study

Cited by 6 publications

References 0 publications

The involvement of neurotrophic factors in the central mechanisms of behavior in adult animals

The involvement of neurotrophic factors in the central mechanisms of behavior in adult animals

The selective effects of a monoclonal antibody against neural growth-related protein A3G7 on central mechanisms of several types of defensive behavior in adult rats

Monoclonal antibodies to A3G7 protein associated with nervous tissue growth disturb learning and memory in adult rats

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