Haemagglutination and Resistance to the Bactericidal Activity of Serum as the Urovirulence Markers of Uropathogenic Escherichia coli

Nachammai, SM; Jayakumar, Karthika; Suresh, Vinithra; Kousalya, M; Aravazhi, Anbu N.

doi:10.20546/ijcmas.2016.509.057

Cited by 5 publications

(4 citation statements)

References 10 publications

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“…A study tested ninetynine E. coli isolates in which 3%, 25% and 1% were strong, moderate and non-biofilm producers. Their results were not comparable to current study due to variations in optical density values of biofilm 44 . In the current study, in-vitro biofilm production was 80% by clinical isolates of E. coli.…”

Section: Discussioncontrasting

confidence: 98%

Role of Biofilm Formation in Mediation of Drug Resistance in E. Coli

Imtiaz¹,

Saleem²,

Ahmad³

et al. 2022

PJMHS

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Aim: Measure biofilm formation in clinical isolates of Escherichia coli and compare the survival of bacteria by treatment of ciprofloxacin, ceftriaxone and amoxicillin/clavulanic acid in the presence and absence of biofilm formation. Methodology: A total of ninety isolates of Escherichia coli were included in the study. All isolates were identified for biofilm production by microtiter assay. Antibiotic susceptibility test for all E. coli strains along with ATCC E. coli 25922 as a control was performed by agar dilution method (CLSI 2016) using three antibiotics e.g. ciprofloxacin, ceftriaxone and amoxicillin/clavulanic acid Result: Out of ninety E. coli isolates, 18(20%) were strong biofilm formers, 54(60%) were moderate and non-biofilm formers were 18(20%). Resistance to ciprofloxacin, ceftriaxone and amoxicillin/clavulanic acid were 75.5%, 63.3% and 22.2% respectively in all tested isolates. The rate of antibiotic resistance of strong biofilm formers, moderate formers and non-biofilm formers were 83.3%, 74% and 77.7% respectively for ciprofloxacin. Similarly, for ceftriaxone resistance pattern of strong, moderate and non-biofilm former were 66.6%, 66.6% and 50% respectively. Strong, moderate and non-biofilm forming E. coli were 11.1%, 27.7% and 22% respectively resistant to amoxicillin/clavulanic acid. Conclusion: Biofilm is not essentially involved in mediating drug resistance in E. coli against tested ciprofloxacin, ceftriaxone and amoxicillin/clavulanic acid. Biofilm formers showed more resistance to antibiotics than non-biofilm formers but statistical analysis of both groups against three antibiotics showed that there was no significant difference of resistance observed among them. There may be another molecular and enzymatic mechanism that allows bacteria to mediate resistance. Keywords: Biofilm formation, antibiotic resistance, minimum inhibitory concentration

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Section: Discussioncontrasting

confidence: 98%

Role of Biofilm Formation in Mediation of Drug Resistance in E. Coli

Imtiaz¹,

Saleem²,

Ahmad³

et al. 2022

PJMHS

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“…Other possible causes include host character factors such as types of infection and predisposing factors that determine the host and pathogen's interaction in vivo. 8 Other studies on the outer membrane protein of 20 kDa K. pneumonia have shown the agglutination of bacterial proteins in the mouse erythrocytes. The agglutination ability is demonstrated by formation of red aggregate points at dilutions of 1/2 to 1/6.…”

Section: Discussionmentioning

confidence: 96%

Characterization of pili protein with molecular mass of 85 kDa Escherichia coli as an adhesin and a hemagglutinin

Agustina

Aisyah

Sutejo

et al. 2020

JKKI

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Background: Escherichia coli (E. coli) is a rod-shaped gram-negative bacterium included in Multi Drug Resistant (MDR) bacteria. There are several strategies in place to prevent and treat E. coli bacterial infections to reduce some the incidences of MDR. One of them is the development of a protein-based vaccine. Pili is one of virulence factors in E. coli surface proteins that can mediate attachments of bacteria to host cells (adhesin or hemagglutinin) and can be used as protein-based vaccine candidates. Objective: This study aims to determine characterization of pili protein with molecular mass of 85 kDa Escherichia coli bacteria as an adhesin and a hemagglutinin. Methods: This study is a true experimental laboratories and a descriptive study to determine roles of pili protein with molecular mass of 85 kDa as an adhesin and a hemagglutinin. Samples used in this study were a stock of E. coli in the Microbiology Laboratory Faculty of Medicine, Universitas Negeri Jember (UNEJ). A hemagglutination test in this study used mouse erythrocytes while an adhesion test used mouse enterocytes. Isolation and purification of E. coli pili protein, isolation of mouse erythrocytes, isolation of mouse enterocytes, the hemagglutination test and the adhesion test were methods in this study. A data analysis with a correlation-regression was used to determine relationships between the adhesion index and titer pili 85 kDa with a limit of significance, 0.05 (p <0.05). Results: The hemagglutination test showed no ability of agglutination with the formation of red aggregate points starting from the first dilution. An analysis with Pearson correlation indicated a relationship between titer pili 85 kDa E. coli and adhesion index (p = 0.009; R = -0.921). Conclusion: Pili protein 85 kDa E. coli plays a role as an adhesin protein and do not have a role as a hemagglutinin protein. Latar Belakang: Escherichia coli (E. coli) merupakan bakteri Gram negatif yang termasuk dalam bakteriMulti Drug Resistant (MDR). Saat ini ada beberapa strategi dilakukan untuk mencegah dan mengobati infeksi bakteri E. coli sehingga mengurangi angka terjadinya MDR. Salah satunya dengan pengembangan vaksin berbasis protein. Pili merupakan salah satu faktor virulensi pada protein permukaan E. coli yang dapat memperantarai perlekatan bakteri dengan sel inang (adhesin atau hemaglutinin) dan dapat dijadikan kandidat vaksin berbasis protein. Tujuan: Penelitian ini bertujuan untuk mengetahui peran pili 85 kDa E. coli sebagai protein adhesin dan hemaglutinin.

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“…Prevalensi E. coli hemolitik isolat asal infeksi saluran kencing pada manusia dilaporkan 25% (Vijyalakshmi et al, 2015), 27% (Divakara et al, 2017;Kaira dan Pai, 2017), 33% (Nachammai et al, 2016), 34% (Tabasi et al, 2015); 41% (Raksha et al, 2003), 45,5% (Mandal et al, 2001), dan 47% (Mittal et al, 2014). Prevalensi E. coli hemolitik isolat kasus ekstraintestinal pada manusia sebesar 9% (Vaish et al, 2016) dan 21% (Kausar et al, 2009).…”

Section: Uji Fagositosis Netrofilunclassified

“…Semua faktor virulensi baik faktor fenotip maupun genotip pada E. coli strain patogen secara bersama-sama maupun sendiri-sendiri dapat menyebabkan terjadinya proses infeksi yang dimulai dari kolonisasi, adhesi, infeksi dan timbulnya gejala penyakit dengan tingkat patogenisitas yang berbeda (Nachammai et al, 2016) Susu mentah dan produk susu olahan merupakan sumber utama penyebaran E. coli ke manusia (Momtaz et al, 2013;Dehkordi et al, 2014). Susu sapi mentah umumnya mengandung E. coli patogen dan non patogen (Hassanshahian dan Shahi, 2014;Ahmed dan Shimamoto, 2014;Younis et al, 2018) perlu pengawasan dalam produksi susu, penanganan setelah pemerahan maupun pengolahannya (Virpari et al, 2013).…”

Section: Uji Fagositosis Netrofilunclassified

Deteksi Faktor Virulensi Secara Fenotip pada Escherichia coli Isolat Susu Mentah Sapi Perah

Khusnan¹,

Purnomo²

2022

jveteriner

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Susu sapi merupakan media pertumbuhan bakteri patogen maupun non patogen. Escherichia coli mrupakan salah satu bakteri yang sering ditemukan pada susu sapi mentah. Escherichia coli patogen pada sapi dapat menyebabkan mastitis maupun radang usus serta dapat menyebabkan masalah kesehatan pada manusia seperti diare, kolitis hemoragik dan sindrom uraemik hemolitik. Hemaglutinin dan hemolisin merupakan faktor virulen yang penting pada E. coli patogen, karena berperan sebagai faktor adhesin, dan faktor pertahanan terhadap fagositosis. Tujuan penelitian ini mendeteksi hemaglutinin dan hemolisin E. coli isolat asal susu sapi mentah serta kemampuan adhesi pada sel epitel serta pertahanan terhadap fagosit netrofil. Hasil penelitian ini menunjukkan 100% isolat E. coli tidak memiliki hemaglutinin dan 17,2% merupakan E. coli hemolitik. Pada uji adhesi mampu melekat pada sel epitel bukalis 46,87 bakteri/sel. Pada uji fagositosis isolat-isolat hemolitik lebih sedikit difagosit oleh neutrofil dibandingkan isolat non hemolitik (2,64 dibanding 3,1 bakteri) tiap neutrofil (P<0,05). Ditemukannya E. coli patogen pada susu mentah menegaskan pentingnya pasteurisasi pada susu sebelum dikonsumsi.

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Haemagglutination and Resistance to the Bactericidal Activity of Serum as the Urovirulence Markers of Uropathogenic Escherichia coli

Cited by 5 publications

References 10 publications

Role of Biofilm Formation in Mediation of Drug Resistance in E. Coli

Role of Biofilm Formation in Mediation of Drug Resistance in E. Coli

Characterization of pili protein with molecular mass of 85 kDa Escherichia coli as an adhesin and a hemagglutinin

Deteksi Faktor Virulensi Secara Fenotip pada Escherichia coli Isolat Susu Mentah Sapi Perah

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