Haematologic effects of leukoreduction on canine whole blood post-filtration and post-storage

Notomi, Marcia Kikuyo; Gopegui, Rafael Ruiz de; Escodro, Pierre Barnabé

doi:10.1007/s00580-015-2155-3

Cited by 5 publications

(5 citation statements)

References 18 publications

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“…Based on the European human guideline for quality of FFP and pRBC recommend a reduction to <1 × 10 6 WBC per Unit (16), which could not be achieved here and in previous studies (38). Leukodepletion by buffy coat separation prior filtering through a leukodepletion filter might be more efficient, but is not possible in this system or study design (22, 39).…”

Section: Discussionmentioning

confidence: 55%

“…A previous human study (13) showed, that hemolysis starts to increase after day 22 of storage reaching >0.8% hemolysis on day 29 demanding further research for canine pRBC. Hemolysis has been evaluated for leukodepleted and non-leukodepleted pRBC in veterinary and human literature showing some evidence of increased hemolysis depending on filter type and material (38, 40, 42–44) emphasizing the need for further studies.…”

Section: Discussionmentioning

confidence: 99%

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Prospective Comparative Quality Control Study of a Novel Gravity-Driven Hollow-Fiber Whole Blood Separation System for the Production of Canine Blood Products

et al. 2019

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The aim of this prospective study was to compare quality of blood products produced either by a novel gravity-driven hollow-fiber separation system (HF) or by centrifugation (C). Whole blood was obtained from 31 healthy non-greyhound canine blood donors and separated into fresh frozen plasma and packed red blood cells using either HF or C in a university teaching hospital. Red blood cell (RBC) count, albumin and fibrinogen concentration, prothrombin time (PT), activated partial thromboplastin time (aPTT) and coagulation factor activity (FV, FVIII), von Willebrand Factor (vWF), and antithrombin activity were assessed. Plasma obtained with the HF showed a significantly higher median PT (9.4 vs. 7.9 s, P = 0.0006) and aPTT (14.9 vs. 13.1 s, P = 0.0128) than plasma prepared with C. Lower albumin (21.7 vs. 23.5 g/l, P = 0.0162) and fibrinogen (1.0 vs. 1.5 g/l, P = 0.0005) concentrations and activities of FV (105 vs. 114%, P = 0.0021) and antithrombin (104 vs. 117%, P = 0.0024) were seen in blood products obtained with the HF. In contrast, vWF was not affected by the method of plasma separation. Compared to HF, RBC count as well as hematocrit were not significantly higher (8.0 vs. 8.9 10 12 /l, P = 0.1308; 0.57 vs. 0.62 l/l, P = 0.0736) when blood products were prepared with C. In conclusion, higher quality of blood products especially regarding coagulation parameters and RBCs was achieved by using C compared to HF. Despite the statistical significances, however, the clinical relevance has to be further elucidated. Nevertheless, HF provides an alternative to produce blood products if a centrifuge is not available.

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Section: Discussionmentioning

confidence: 55%

Section: Discussionmentioning

confidence: 99%

Prospective Comparative Quality Control Study of a Novel Gravity-Driven Hollow-Fiber Whole Blood Separation System for the Production of Canine Blood Products

et al. 2019

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“…Removal of these cells mitigates some of the effects associated with stored blood transfusion, including febrile non-haemolytic transfusion reactions, and infection. 23,30,31 A pilot study showed that prestorage leukoreduction of FWB units is possible using an in-line neonatal filter. 32 However, the main limitation of this technique is the reduction in blood volume, as the residual filter volume is 8 ml.…”

Section: Discussionmentioning

confidence: 99%

Haematological and morphological evaluation of feline whole blood units collected for transfusion purposes

Spada

Perego

Baggiani

et al. 2018

Journal of Feline Medicine and Surgery

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Objectives Despite the increasing availability of feline blood collected and stored for transfusion purposes, few studies have been performed on feline blood units. The aim of this prospective in vitro study was to evaluate haematological and morphological changes in feline blood cells in whole blood units between collection and end of storage. Methods Haematological examination (red blood cells [RBCs], haemoglobin, haematocrit, red cell distribution width, mean cell volume, mean cell haemoglobin concentration, mean cell haemoglobin, white blood cells [WBCs] and platelet [PLT] count) was performed on 40 non-leukoreduced feline whole blood units at time of collection (day[D]0) and after storage (D35). The blood was collected into citrate-phosphate-dextrose-adenine anticoagulant-preservative solution using an open system in a veterinary blood bank and stored for 35 days at 4 ± 2°C. Twenty of these feline whole blood units were also analysed for blood cell morphology (normal RBCs, macrocytes, echinocytes, spherocytes, schistocytes, lysed RBCs, RBCs with Heinz bodies and recognisable WBC and PLT count). Differences between the two examination times were statistically analysed. Results There was a statistically significant decrease in WBC and PLT counts after storage at D35 ( P <0.0001 for both). The most significant cellular morphological change after storage was an increase in echinocyte count ( P = 0.0001), and lysed RBCs ( P <0.0001), and a decrease in normal RBCs ( P <0.0001). Recognisable WBCs - mainly lymphocytes - were present at the end of storage. Conclusions and relevance This study showed that significant morphological changes occur in RBCs in feline blood units during storage for 35 days. In vivo studies are required to establish if these changes could affect the ability of stored RBCs to circulate and provide adequate oxygen delivery after transfusion.

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“…Although research focusing on animal blood therapy has received some attention in recent years, with research available about blood preservation and transfusion in a wide range of animal species [ 15 , 16 , 17 , 18 , 19 ], the basic understanding of physiological alteration occurring after acute blood loss are still limited, especially in ruminants. Thus, this study aims to evaluate the clinical, hematological, biochemical, and oxidative stress changes in sheep subjected to acute blood loss.…”

Section: Introductionmentioning

confidence: 99%

Impact of Acute Blood Loss on Clinical, Hematological, Biochemical, and Oxidative Stress Variables in Sheep

Sousa

Oliveira

et al. 2022

Veterinary Sciences

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Blood loss in sheep can have different causes and may result in anemia. We aimed to evaluate the clinical, hematological, and biochemical alterations and the oxidative stress generated by acute blood loss. Eighteen healthy sheep underwent phlebotomy to remove 40% of the blood volume and were evaluated clinically and by laboratory tests for clinical, biochemical, and blood gas variables and to assess oxidative stress before induction (T0), 30 min (T30 min), and 6 (T6 h), 12 (T12 h), and 24 h (T24 h) after blood loss. The sheep showed tachycardia from T30 min until T24 h, reduction in the hematocrit, number of erythrocytes, and hemoglobin concentration, with lower values at T24 h and increase in the number of leukocytes from T12 h on. There was a reduction in blood pH and oxygen pressure at T30 min, increased lactate concentration and reduced blood bicarbonate at this time. There was an increase in urea concentration from T6 h until the end of the study, with no change in creatinine levels. The animals did not show changes in the concentration of malonaldehyde, and in the activity of the enzymes superoxide dismutase, glutathione peroxidase, and catalase, but there was a reduction in the concentration of reduced glutathione at T24 h. The acute loss of 40% of blood volume is capable of promoting relevant clinical, hematological, blood gas, and biochemical alterations, and contributed to the appearance of oxidative stress with reduced glutathione concentration, suggesting that this process generated free radicals in sufficient quantity to diminish the action of antioxidants.

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Haematologic effects of leukoreduction on canine whole blood post-filtration and post-storage

Cited by 5 publications

References 18 publications

Prospective Comparative Quality Control Study of a Novel Gravity-Driven Hollow-Fiber Whole Blood Separation System for the Production of Canine Blood Products

Prospective Comparative Quality Control Study of a Novel Gravity-Driven Hollow-Fiber Whole Blood Separation System for the Production of Canine Blood Products

Haematological and morphological evaluation of feline whole blood units collected for transfusion purposes

Impact of Acute Blood Loss on Clinical, Hematological, Biochemical, and Oxidative Stress Variables in Sheep

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