The aim of this study was to characterize tinctures and microcapsules loaded with an ethanol extract of red propolis through chemical, physicochemical and microbiological assays in order to establish quality control tools for nutraceutical preparations of red propolis. The markers (isoflavonoids, chalcones, pterocarpans, flavones, phenolic acids, terpenes and guttiferones) present in the tinctures A and B were identified and confirmed using LC/ESI/FTMS/Orbitrap. Four compositions (A, B, C and D) were prepared to contain B tincture of the red propolis with some pharmaceutical excipients and submitted to two drying processes, i. e. spray-drying and freeze-drying to obtain microcapsules loaded with the red propolis extract. The tinctures and microcapsules of the red propolis were submitted to the total flavonoid content and antioxidant activity tests. The antibacterial activity and minimum inhibitory concentration (MIC) were tested using Staphylococcus aureus ATCC 25293 and Pseudomonas aeruginosa ATCC 27853 strains. The tinctures and microcapsules presented high flavonoid quantities from 20.50 to 40.79 mg/100 mg of the microcapsules. The antioxidant activity and IC50 were determined for the tinctures A and B (IC50: 6.95 µg/mL and 7.48 µg/mL), the spray-dried microcapsules (IC50: 8.89–15.63 µg/mL) and the freeze-dried microcapsules (IC50: 11.83–23.36 µg/mL). The tinctures and microcapsules were proved to be bioactive against gram-positive and gram-negative bacteria with inhibition halos superior to 10 mm at concentration of 200 µg/mL and MIC values of 135.87–271.74 µg/mL using gram-positive strain and 271.74–543.48 µg/mL using gram-negative strain. The tinctures and microcapsules of the red propolis have a potential application for nutraceutical products.

BackgroundPropolis is a natural substance produced by bees and is known to have antimicrobial activity. Our aim was to evaluate the antimicrobial effect of micellar nanocomposites loaded with an ethyl acetate extract of Brazilian red propolis as a cavity cleaning agent and its influence on the color and microtensile bond strength (μTBS) of the dentin/resin interface.MethodsAn ultra-performance liquid chromatography coupled with a diode array detector (UPLC-DAD) assay was used to determine the flavonoids and isoflavones present in an ethyl acetate extract of Brazilian red propolis (EARP) and micellar nanocomposites loaded with EARP (MNRP). The antimicrobial activity of EARP and MNRP was tested against Streptococcus mutans, Lactobacillus acidophilus, and Candida albicans. One of the following experimental treatments was applied to etched dentin (phosphoric acid, 15 s): 5 μL of MNRP (RP3, 0.3%; RP6, 0.6%; or RP1, 1.0% w/v), placebo, and 2% chlorhexidine digluconate. Single Bond adhesive (3 M/ESPE) was applied and a 4-mm-thick resin crown (Z350XT, 3 M/ESPE) was built up. After 24 h, the teeth were sectioned into sticks for the μTBS test and scanning electron microscopy. Spectrophotometry according to the CIE L*a*b* chromatic space was used to evaluate the color. Data were analyzed using one-way ANOVA and the Tukey test or Kruskal-Wallis test and the same test for pairwise comparisons between the means (P < 0.05).ResultsThe UPLC-DAD assay identified the flavonoids liquiritigenin, pinobanksin, pinocembrin, and isoliquiritigenin and the isoflavonoids daidzein, formononetin, and biochanin A in the EARP and micellar nanocomposites. EARP and MNRP presented antimicrobial activity against the cariogenic bacteria Streptococcus mutans and Lactobacillus acidophilus, and for Candida albicans. ΔE values varied from 2.31 to 3.67 (P = 0.457). The mean μTBS for RP1 was significantly lower than for the other groups (P < 0.001). Dentin treated with RP1 showed the shortest resin tags followed by RP6 and RP3.ConclusionsThe EARP and (MNRP) showed antimicrobial activity for the main agents causing dental caries (Streptococcus mutans and Lactobacillus acidophilus) and for Candida albicans. MNRP at concentrations of 0.3 and 0.6% used as a cavity cleaner do not compromise the aesthetics or μTBS of the dentin/resin interface.

Foi estudada a neurólise química dos nervos Palmares em 10 eqüinos. Usando-se uma ferradura especial para induzir claudicação, a locomoção avaliada ao passo e trote e os graus foram registrados antes e após a injeção perineural do álcool benzílico 0,75% (Grupo A) e o álcool etílico absoluto (Grupo B). Os testes mostraram que ambos os neurolíticos dessensibilizaram completamente a sola dos animais a partir de 30 dias, assim permanecendo até o 150º dia nos animais do Grupo A, quando ocorreu retorno progressivo da sensibilidade. Já no Grupo B, aos 180 dias, a sensibilidade permanecia ausente. A análise histopatológica revelou que nos nervos infiltrados com álcool benzílico 0,75% prevaleceram lesões próprias de axonotemese, sugerindo tendência de regeneração e restabelecimento da condução nervosa, enquanto que nos nervos infiltrados com álcool etílico absoluto, prevaleceram lesões simuladoras de neurotemese, com degeneração nervosa permanente. Concluiu-se que os álcoois utilizados foram eficientes bloqueadores temporários ou permanentes dos nervos Palmares em eqüinos.

The present study identified the main endoparasites present in donkeys (Equus asinus) used in skin exploitation located in Cando municipality, Bahia State, Northeast of Brazil. The samples were collected from September 2019. Feces were collected from the rectal ampulla of 34 animals, macroscopically visualized for parasitic forms, and microscopically evaluated to identify endoparasites forms using the McMaster method. Parasitological results were associated with sex, age, and bodyweight Stata Corp LLC 14. Endoparasites were found in 82.3% of the animals, with the egg count ranging from 50 to 1050 eggs per gram (EPG). The sole presence of superfamily Trichostronglylidae was observed in 67.6% of the donkeys, in 8.8% co-infected by Trichostronglylidae and Eimeria spp., while Trichostronglylidae and Strongyloides westeri was detected in 2.9%, and simultaneous infection by Trichostronglylidae, Strongyloides westeri, and Oxyuris equi was observed in 2.9%. The occurrence of parasitic infections varies according to nutritional status, age, sex, and environmental exposure (p>0.05). A high occurrence of infection was observed in young animals and those with lower body weight.

RESUMO:Milhares de pessoas utilizam a carroça tracionada por equídeos como meio de trabalho ou suplementação de renda no âmbito mundial. Poucas são as pesquisas envolvendo a caracterização biométrica dos animais e as medidas dos equipamentos de atrelagem, buscando classificar os equídeos pela capacidade de tração, propondo discussões cientificamente embasadas acerca da capacidade de carga por animal, consequentemente diminuindo os maus tratos e dimensionando o trabalho de forma proporcional e coerente. Objetivou-se determinar parâmetros zoométricos ligados a ezoognósia, caracterizando tipologia e medidas relacionadas à veículos de atrelagem de 234 equídeos de tração no município de Arapiraca-Alagoas, Brasil. Foram obtidas medidas morfoestruturais lineares e perimetrais, calculando-se os índices zoométricos: índice corporal, peso estimado, índice dáctilo-torácico, índice de carga a passo e índice de carga a galope. Os dados foram submetidos a uma análise de variância e teste de médias (Skott-Knott a 5% de probabilidade). Os muares representaram 76% (179) dos animais estudados, os equinos 21% (49) e os asininos 3% (6). Em todas as medidas morfoestruturais e índices zoométricos, com exceção do índice corporal onde os asininos apresentaram a maior média, verifica-se diferença (P<0,05) entre as espécies com os equinos sempre apresentando maiores valores, seguidos pelos muares e asininos. Não há adequação em relação às medidas das atrelagens, verificando-se diferença estatística (P<0,05) apenas para os veículos tracionados por asininos. Os equinos e muares utilizados por condutores de carroças são classificados como equídeos de pequeno porte, com propensão à tração leve. Palavras-chave: carroça; equídeos urbanos; índices; medidas BIOMETRIC STANDARD, WAGON MEASURES AND LOAD INDEX OF URBAN CART EQUIDS OF ARAPIRACA CITY, ALAGOASABSTRACT: Thousands of people use the cart pulled by horses as a means of work or supplemental income in the world. But there are few studies involving the animal biometric characterization and wagon measures, seeking classify equids for traction capacity and propose scientifically sound discussions about the load capacity per animal, thus reducing maltreatment. The aim of this paper was to determine the zoometric standard, typology and wagon measures of the 234 cart equids from Arapiraca city, Alagoas state, Brazil. Morphostructural linear and perimeter measurements were obtained, calculating the zoometric indexes: body index, estimated weight, dactyl-thoracic index, work walking index and work galloping index. The variables were subjected to analysis of variance and means test (Skott-Knott 5% probability). The mules represented 76% (179) of the animals, horses 21% (49) and donkeys 3% (6). In all measurements and indexes studied, except where the donkeys body index had the highest average, there is a statistical difference (P <0.05) between species with the highest values always with horses, mules and followed by donkeys. There are no adequate in relation to the wagon measures, verifying differen...