Haematological and immunological reference intervals for adult population in the state of Amhara, Ethiopia

Enawgaw, Bamlaku; Birhan, Wubet; Abebe, Molla; Terefe, Betelihem; Baynes, Habtamu Wondifraw; Deressa, Tekalign; Melku, Mulugeta

doi:10.1111/tmi.13071

Cited by 16 publications

(28 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The RIs established in the proposed investigation for non-pregnant women deviated from those established in other countries, as shown in annex-II. The current study's upper and lower RI limits for WBC were higher than those found in earlier studies from Amhara region [24], Northwest Morocco [59], and Nigeria (RI) [21] but comparable to those found in Dire Dawa [60]. In this study, the lower RI limit of Hgb was higher than the lower RI limit of studies done in Dire Dawa [60], Amhara [24] and Northwest Morocco [59], whereas the upper RI limit was lower than other studies [24,61].…”

Section: Plos Onecontrasting

confidence: 92%

“…The current study's upper and lower RI limits for WBC were higher than those found in earlier studies from Amhara region [24], Northwest Morocco [59], and Nigeria (RI) [21] but comparable to those found in Dire Dawa [60]. In this study, the lower RI limit of Hgb was higher than the lower RI limit of studies done in Dire Dawa [60], Amhara [24] and Northwest Morocco [59], whereas the upper RI limit was lower than other studies [24,61]. The lower RI limit of RBC in the current study was higher than in previous studies in Dire Dawa [60], Amhara [24], Asmara [61], Northwest Morocco [59], Barcelona [62], and textbook [63], but it was comparable with Nigeria [21].…”

Section: Plos Onecontrasting

confidence: 92%

“…This is because pregnancy places intense stress on the hematological system and several quantitative changes happen [17,18]. These complications and physiological changes observed in pregnant women need accurate and reliable hematological parameter RIs [19][20][21] Even if there is a common pregnancy-induced laboratory abnormality (mainly anemia and thrombocytopenia) and significant variations between women and men are well documented, very few laboratories routinely provide reference values for pregnant and non-pregnant women [8,[22][23][24][25][26]. Indeed, many laboratories in Ethiopia have been using RIs established from non-Ethiopian participants, mostly from western countries which are available in textbooks, inserted leaflet or manufacturer manual [26].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Common hematological parameters reference intervals for apparently healthy pregnant and non-pregnant women of South Wollo Zone, Amhara Regional State, Northeast Ethiopia

et al. 2022

View full text Add to dashboard Cite

Background Hematological reference intervals (RIs) are affected by inherent variables like age, sex, genetic background, environment, diet and certain circumstances such as pregnancy signifying the need for population specific values. This study was designed to establish RIs for common hematological parameters of apparently healthy pregnant and non-pregnant women from Northeast Ethiopia. Method This community based cross-sectional study recruited 600 pregnant and non-pregnant women in South Wollo Zone, Northeast Ethiopia from June to August 2019. Complete blood count was performed for eligible participants using Mindary BC-3000 plus hematology analyzer. The mean, median, and 2.5th and 97.5th percentile reference limits with 90% CI were determined using SPSS version 23. Result The established selected 2.5th–97.5th percentiles RIs for pregnant women were: WBC: 4.0–13.2x109/L; RBC: 3.45–4.67x1012/L; Hgb: 10.1–13.7g/dL; HCT: 33.5–46.5%; MCV: 85-104fL; MCH: 27.5–33.0pg; MCHC: 30.3–33.7g/dL and Platelet count: 132-373x109/L. The respective values for non-pregnant women were 3.6–10.3; 4.44–5.01; 12.4–14.3; 38.4–50.1; 86–102; 27.1–32.4, 30.4–34.1, 173–456. A statistically significant difference between pregnant and non-pregnant women was noted in all hematological parameters except MCHC. The mean and median value of WBC count, MCV, MPV and PDW increased, whereas mean values of HCT and Platelet count decreased as gestational period advances. Conclusion The observed difference from other studies signify the necessity for using trimester specific RIs and separate RI for pregnant and non-pregnant women. Thus, we recommend the health facilities in the study area to utilize the currently established RIs for pregnant and non-pregnant women for better care.

show abstract

Section: Plos Onecontrasting

confidence: 92%

Section: Plos Onecontrasting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Common hematological parameters reference intervals for apparently healthy pregnant and non-pregnant women of South Wollo Zone, Amhara Regional State, Northeast Ethiopia

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Immunological status of the study participants, as favorable (> 400 cells/mm 3 for male and > 466 cells/mm 3 for female) and unfavorable (< 400 cells/mm 3 for male and < 466 cells/mm 3 for female), was defined based on the reference interval of CD4 + cell count established for the population residing northwest Ethiopia [28]. Virological suppression was considered when the HIV-RNA level is < 1000 copies/μl at any point after 6 months of treatment with HAART [29].…”

Section: Operational Definitionmentioning

confidence: 99%

Immunological status and virological suppression among HIV-infected adults on highly active antiretroviral therapy

Melku

Abebe

Teketel

et al. 2020

Environ Health Prev Med

Self Cite

View full text Add to dashboard Cite

Background: World Health Organization (WHO) recommends that viral load ([VL) is a primary tool that clinicians and researchers have used to monitor patients on antiretroviral therapy (ART), an antiviral drug against retroviruses. Whereas, CD4 cell counts can only be used to monitor clinical response to ART in the absence of VL testing service. Therefore, this study is aimed to assess the level of immunological status and virological suppression, and identify associated factors among human immunodeficiency virus ([HIV)-infected adults who were taking antiretroviral drugs of combination regimen know as highly active antiretroviral therapy (HAART). Methods: A hospital-based cross-sectional study was conducted at the University of Gondar comprehensive specialized referral hospital from February to April 2018. A total of 323 adult participants on HAART were selected using a systematic random sampling technique and enrolled into the study. Blood samples for viral load determination and CD4 cell count were collected. Binary logistic regression analysis was used to determine factors associated with immunologic status and virological suppression in HIV patients on HAART. Odds ratio with 95% CI was used to measure the strength of association. Results: Virological suppression (VL level < 1000 copies/ml) was found in 82% (95% CI 77.7, 86.1) of study participants, and it has been associated with CD4 cell count between 350 and 499 cells/mm 3 (adjusted odds ratio (AOR) = 2.56; 95% CI 1.14, 5.75) and > 499 cells/mm 3 (AOR = 7.71; 95% CI 3.48, 17.09) at VL testing and current age > 45 years old (AOR = 5.99; 95% CI 2.12, 16.91). Similarly, favorable immunological status (≥ 400 cells/mm 3 for male and ≥ 466 cells/mm 3 for female) was observed in 52.9% (95% CI 47.4, 58.8) of the study participants. Baseline CD4 cell count of > 200 cells/mm 3 , age at enrollment of 26 through 40 years old, and urban residence were significantly associated with favorable immunological status. Conclusion: Though the majority of HIV-infected adults who were on HAART had shown viral suppression, the rate of suppression was sub-optimal according to the UNAIDS 90-90-90 target to help end the AIDS pandemic by 2020. Nonetheless, the rate of immunological recovery in the study cohort was low. Hence, early initiation of HAART should be strengthened to achieve good virological suppression and immunological recovery.

show abstract

“…Hematological and immunological reference intervals among different groups of the Ethiopian population are poorly defined. Few studies conducted in Ethiopia among apparently healthy individuals attending health institutions indicated that the presence of significant differences with those of the western, Asian, and most African countries [25][26][27][28].…”

Section: Introductionmentioning

confidence: 99%

Hematological and CD4+ T- cell count reference interval for pregnant women attending antenatal care at Hawassa University Comprehensive Specialized Hospital, Hawassa Southern Ethiopia

et al. 2021

View full text Add to dashboard Cite

Background Pregnancy is a state characterized by physiological, hematological, and immunological changes. However, the reference intervals (RI) being used in clinical practice in Ethiopia are derived from non-local general populations. Therefore; this study was aimed to determine the reference interval of hematological and immunological profiles among healthy pregnant mothers attending Hawassa University Hospital. Methods A cross-sectional study in a total of 360 healthy pregnant women was enrolled from January to April 2019, at Hawassa University hospital. Sociodemographic and obstetric data were collected using a structured questionnaire. Blood samples collected from each participant were used to define the hematological parameters. The median and 95% intervals were calculated for the immunological and hematological profiles. P-value 0.05 was considered statistically significant. Result A total of 360 healthy pregnant women were enrolled in this study. The age range of the participants was 18–45 years. 342(95%) were married and 270 (75%) of the participants were multigravida. The overall median CD4+ T-cell and total WBC counts (cells/mm3) were 602 and 7.58 respectively. The overall median value for lymphocytes, neutrophils, monocytes, eosinophils, and basophil count was (cells/mm3) was 2.21, 6.74, .63, .53, and 0.09 respectively. Whereas the median RBC and platelet count was 4.48×106/μLand 212×106/μL. The median value of hematological profiles in the first, second, and third trimesters was TWBC (103/μL) (7.90, 8.30, 8.65), RBC (106/μL) (4.5, 4.6, 4.62), and PLT (103/μL) (210, 209,161) respectively. The CD4 T cell count median value was (600, 598, and 591) in the first, second, and third trimesters. Significant changes were observed in hematological and immunological parameters between trimesters (P < 0.05). Conclusion Significant changes were observed in hematological and immunological parameters between trimesters (P < 0.05). Considerable differences were also seen between the values in this study and other studies from Ethiopia and other countries, indicated the need for the development of local reference intervals for pregnant women.

show abstract

Haematological and immunological reference intervals for adult population in the state of Amhara, Ethiopia

Abstract: The reference intervals established in this study differ from others and thus should be used for the interpretation of laboratory results in diagnosis and safety monitoring in clinical trials in Amhara.

Cited by 16 publications

References 38 publications

Common hematological parameters reference intervals for apparently healthy pregnant and non-pregnant women of South Wollo Zone, Amhara Regional State, Northeast Ethiopia

Common hematological parameters reference intervals for apparently healthy pregnant and non-pregnant women of South Wollo Zone, Amhara Regional State, Northeast Ethiopia

Immunological status and virological suppression among HIV-infected adults on highly active antiretroviral therapy

Hematological and CD4+ T- cell count reference interval for pregnant women attending antenatal care at Hawassa University Comprehensive Specialized Hospital, Hawassa Southern Ethiopia

Contact Info

Product

Resources

About