Haematology of gynogenetic diploids of Siberian sturgeon Acipenser baerii Brandt

Abstract

Haematological analyses were conducted in gynogenetic diploids and control diploids of Siberian sturgeon (Acipenser baerii). Gynogenetic diploid fish were obtained from Polish aquaculture after induction of meiotic gynogenesis based on application of UV-irradiated hybrid Siberian sturgeon × Russian sturgeon (A. gueldenstaedtii) sperm (for eggs activation) and heat shock for inhibiting the second polar body retention. A total of fifteen 30-month-old specimens of Siberian sturgeon from the gynogenetic diploid group and 12 specimens from the control diploid group were randomly sampled. There were no differences in the number of erythrocytes and haematocrit values between gynogenetic diploids and normal diploids of Siberian sturgeon. However, the percentage of normal erythrocytes was lower in the gynogenetic group of fish and the higher percentage of the atypical red blood cell forms (microcytes, sphaerocytes, erythrocytes with displaced nuclei and with divided nuclei) were observed in this group of fish. Additionally all the haemoglobin indices (haemoglobin concentration, the mean corpuscular haemoglobin and the mean corpuscular haemoglobin concentration) were significantly reduced in gynogenetic diploids of Siberian sturgeon. These results could suggest depletion of oxygenation capacity of the blood of gynogenetic sturgeons. This is the first report of a haematological study in gynogenetic diploids of sturgeon fish.

Examination of white blood cell indicators for three different ploidy level sturgeon species reared in an indoor recirculation aquaculture system for one year

“…The eosinophils were present in lower percentages and constituted 5.6-6.0% from all the leukocytes. Our findings on the granulocytes were in accordance with other studies (Palikova et al 1999;Pourgholam and Saeidi 2000;Flajshans and Vajcova 2002;Ruchin 2007;Zexia et al 2007;Zarejabad et al 2009;Docan et al 2012;Matsche and Gibbons 2012;Akrami et al 2013;Fopp-Bayat et al 2013;Mazandarani et al 2015;Rozynski et al 2015;Adel et al 2016). The dependence between the increasing number of nuclear segments both in neutrophils and eosinophils and increasing ploidy level was statistically significant.…”

“…The reports about the monocytes in the differential counts were often conflicting; some authors did not mention any monocytes in the differential counts at all (Wlasow and Fopp-Bayat 2011;Fopp-Bayat et al 2013), other authors pointed out the sporadic occurrence of monocytes (Palikova et al 1999).…”

Effects of herbs and spice on health status of tilapia (Oreochromis mossambicus) challenged with Streptococcus iniae

“…Red blood cells (RBC, 10 6 mm 3 ), white blood cells (WBC, 10 3 mm 3 ), haematocrit (PCV, %) and haemoglobin (Hb, g/ dl) were determined using standard methods according to Foop-Bayat et al (2013) and Zusková et al (2013). Differential leukocytes were examined in May-Grunwald-Giemsa-stained peripheral blood smears; each slide was examined under oil-immersion at × 100 and for each slide, 100 leukocytes were identified as lymphocytes, neutrophils or monocytes.…”

Effect of concrete pond and net-cage culture systems on growth performance and haematological parameters of Siberian sturgeon (Acipenser baerii)

“…Ghiasi et al (35), in their study on the Acipenser ruthenus (698.6 ± 8.9 g), determined these values as RBC (× 10 5 mm -3 ) 1.32 ± 0.05, Hct (%) 27.0 ± 1.3, Hb (g dL -1 ) 6.3 ± 0.3, MCH (pg cell -1 ) 49.1 ± 3.6, MCHC (g dL -1 ) 24.2 ± 1.9, MCV (fl) 20.7 ± 1.3, WBC (× 10 3 mm -3 ) 62.3 ± 7.5, lymphocyte (%) 91.7 ± 1.4, neutrophil (%) 6.8 ± 1.2, eosinophil (%) 0.8 ± 0.3, and monocyte (%) 0.3 ± 0.1. In one study, RBC (T•L -1 ), Hct (L•L -1 ), Hb (g•L -1 ), MCV (fl), MCH (pg), and MCHC (L•L -1 ) values of the Siberian sturgeon (Acipenser baerii) control group, with an average body weight of 401 g, were found as 0.87 ± 0.02, 0.32 ± 0.02, 72.75 ± 1.69, 361.34 ± 9.57, 83.46 ± 1.51, and 0.23 ± 0.01, respectively [36]. Jahanbakhshi et al [37] stated that the haematological values of the juvenile Great sturgeon (Huso huso) in fiberglass tanks with a capacity of 400 L at 22 ± 1 °C were 6.8 ± 0.23 for Hb (g/dL), 22.1 ± 00 for Hct (%), 12.5 ± 1.52 for WBC (× 10 3 /mm 3 ), 65.4 ± 27.0 for RBC (× 10 4 /mm 3 ), 29.6 ± 3.00 for MCHC (g dL −1 ), 99.8 ± 3.45 for MCH (Pg), 329.21 ± 10.91 for MCV (fl), 6.00 ± 00 for neutrophil (%), 93.3 ± 0.57 for lymphocyte (%), and 0.667 ± 0.57 for eosinophil (%).…”