Haematopoietic effects of Angelica sinensis root cap polysaccharides against lisinopril-induced anaemia in albino rats

Abstract

Context: Angelica sinensis L. (Umbelliferae) has medicinal properties. Objectives: The present study evaluates the haematopoietic effects of A. sinensis polysaccharides (ASP) against lisinopril-induced anaemia. Materials and methods: Thirty healthy adult male albino rats were randomly divided into five groups (n ¼ 6). Group I was control group. Group II was treated with angiotensin-converting enzyme inhibitor (ACEI, 20 mg/kg/day) to induce anaemia. In group III, erythropoietin (EPO, 100 IU/kg/each) was administered in combination with ACEI. Group IV was treated with ASP (1 g/kg/day), extracted from A. sinensis root caps. In Group V, ASP (1 g/kg/day) was treated with ACEI. After 28 days, blood and tissue samples were collected for haematological and histopathological analysis, respectively. Results: The results showed that ACEI significantly reduced the haemoglobin (Hb, 10.0 g/dL), packed cell volume (PCV, 39.5%), red blood cells (RBCs, 6.2 million/mm 3 ), mean corpuscular volume (MCV, 53.5 fL) and mean corpuscular haemoglobin (MCH, 16.2 pg/cell) values. In the group treated with ASP, the Hb (13.7 g/dL) and RBCs (7.8 million/mm 3 ) increased significantly (p < 0.05). The combination of ASP and ACEI led to the significant (p < 0.05) reduction in Hb (10.7 g/dL), PCV (33.3%), RBCs (6.0 million/mm 3 ), MCV (54.42 fL) and MCH (16.44 pg/cell) values. While histopathological examination of the liver and kidney cells showed a mild degree of toxicity in the ASP-treated group. Conclusion: ASP has a potentiating effect on haematological parameters when given alone. However, when administered simultaneously with lisinopril, it showed an unfavourable effect with more complicated anaemia so it should not be used with ACEIs. ARTICLE HISTORY

Finding inhibitors for PCSK9 using computational methods

“…Erythropoiesis can be inhibited by decreasing the level of angiotensin II. ACEi also inhibit the renin-angiotensin system [ 47 ].…”

Chinese Herbal Medicine Significantly Impacts the Haematological Variables of the Athlete Biological Passport

“…The haematopoietic effect of ASR could be mainly ascribed to the water-soluble fraction because the polysaccharides isolated from ASR were found to restore the HGB levels and promote bone marrow haematopoiesis in different blood-deficient rodent models [ 14 , 21 ] and a clinical case of haemodialysis patient [ 22 ]. The underlying mechanisms were demonstrated as the ASR polysaccharides stimulate erythropoietin production and improve iron availability via activating janus kinase 2 (JAK2)/signal transducer and activator of transcription 5 (STAT5) and phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt) signalling pathway [ 13 , 23 , 24 ].…”

“…On the other hand, CHMs frequently applied to patients for improving the symptoms associated with anaemia, Angelicae Sinensis Radix and Astragali Radix for instance, have been demonstrated to regulate haematopoiesis by a number of previous works [ 13 , 14 , 15 ]. These CHMs are not included in the prohibited list nor should contain any banned substances.…”

The Bioactivities and Potential Clinical Values of Angelica Sinensis Polysaccharides

“…33 Liu et al suggested that the hematopoietic and thrombopoietic effects of ASP may result from the phosphatidylinositol 3-kinase/protein kinase B (PI3K/Akt) pathways. 34 In addition, ASP can increase the number of red blood cells and the content of hemoglobin [35][36][37] by up-regulating theexpression of cyclin D1 and Bcl-2 protein. 38 At the same time, ASP can stimulate EPO secretion and regulate the pathway of erythropoiesis, and so the level ofhepcidin mRNA decreased with the increase in erythropoiesis rate.…”