Haemocyte variations in 35 species of grasshoppers and locusts

Abstract

Introduction: Grasshoppers and locusts are widely distributed worldwide, causing significant losses in agriculture. The origin and functions of their haemocytes are not entirely understood. Objectives: Insect haemocytes arbitrate cellular defence and participate in humoral defences. Due to their importance, the haemocytes of 35 species of grasshoppers and locusts from China were morphologically examined in this study. We aim to highlight a simple method for the morphological examination of insect haemocytes. Methods: The haemocytes were observed, counted and compared under a light microscope after Wright-Giemsa staining. Results: High complexity in form and shape were observed in the haemocytes. These include prohaemocytes, plasmatocytes, granulocytes, vermicytes, podocytes and megakaryocytes. No clear relationship was seen between the haemocyte type and their phylogenetic relationship among the three families examined. The high abundance of plasmatocytes and granulocytes suggests their importance in the immunity of grasshoppers and locusts. The minor haemocyte populations including prohaemocytes, vermicytes and podocytes may not be always present in individuals. Conclusion: All examined species shared similarities in their haemocyte types. Wright-Giemsa staining is a simple and efficient method for evaluating haemocytes.

Application of Morphogo based on convolutional neural network for morphological identification of bone marrow nucleated cells

“…The diseases included in each group are as follows: G1 -acute myeloid leukemia (AML), acute lymphoblastic leukemia (ALL), myelodysplastic syndrome transformation into acute leukemia (MDS-AL), chronic myeloid leukemia diagnosed in accelerated phase (CML-AP), and lymphoma; G2 -megaloblastic anemia (MA), plasma cell myeloma (PCM), myelodysplastic syndrome (MDS), chronic myeloid leukemia (CML), and myeloproliferative neoplasms (MPN); G3 -immune thrombocytopenia (ITP), chronic lymphoblastic leukemia (CLL) and other diseases with slight changes in cell morphology; G4 -iron de ciency anemia (IDA), secondary anemia (SA), secondary leukaemia (SL), and aplastic anaemia (AA); and G5 -BM dilution cases. All BM smears were well stained using the Wright-Giemsa method, and the quality of both the smear and staining met the recommendations outlined in the nation guide to clinical laboratory procedures (NGCLP, fourth edition) or by the international council for standardization in hematology (ICSH) [8,21]. The study received approval from the institutional review committee of all participating agencies.…”

Single-Cell Atlas Reveals the Hemocyte Subpopulations and Stress Responses in Asian Giant Softshell Turtle during Hibernation

“…Hemocytes have significant roles in homeostasis maintenance and immunity and coagulation [1][2][3], and research on hemocytes can provide a better understanding of the relationship between their normal and pathological functions and animal growth and development, mature reproduction, living environment and other factors from the perspective of comparative hematology and comparative immunology [4][5][6][7]. Moreover, such research has also explored the trends and characteristics of hemocytes in phylogenetic evolution [8]. The earliest research on reptile hematology, including on the microscopic structure, number, and size of hemocytes, can be traced back to the 1940s [9][10][11].…”