Haemolytic Activity of Asbestos and other Mineral Dusts

Macnab, G M; Harington, J. S.

doi:10.1038/214522a0

Cited by 92 publications

(22 citation statements)

References 8 publications

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“…(59) Asbestos is known to cause hemolysis. (65) We confirmed this property using UICC asbestos and silica (Fig. 3a,b).…”

Section: Resultssupporting

confidence: 68%

“…Hemoglobin is a major oxygen-transporting protein and is released from red blood cells during hemolysis. Adsorption of hemoglobin on the asbestos surface has been mentioned previously, (65) but the present study is the first to demonstrate that this event augments asbestos-induced free radical generation (Figs 3c,4c). We also investigated the direct interaction of red blood cells ⁄ hemoglobin and asbestos fibers by immunohistochemistry (Fig.…”

Section: Discussionmentioning

confidence: 61%

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Asbestos surface provides a niche for oxidative modification

et al. 2011

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Asbestos is a potent carcinogen associated with increased risks of malignant mesothelioma and lung cancer in humans. Although the mechanism of carcinogenesis remains elusive, the physicochemical characteristics of asbestos play a role in the progression of asbestos-induced diseases. Among these characteristics, a high capacity to adsorb and accommodate biomolecules on its abundant surface area has been linked to cellular and genetic toxicity. Several previous studies identified asbestos-interacting proteins. Here, with the use of matrix-assisted laser desorption ionization-time of flight mass spectrometry, we systematically identified proteins from various lysates that adsorbed to the surface of commercially used asbestos and classified them into the following groups: chromatin ⁄ nucleotide ⁄ RNA-binding proteins, ribosomal proteins, cytoprotective proteins, cytoskeleton-associated proteins, histones and hemoglobin. The surfaces of crocidolite and amosite, two iron-rich types of asbestos, caused more protein scissions and oxidative modifications than that of chrysotile by in situ-generated 4-hydroxy-2-nonenal. In contrast, we confirmed the intense hemolytic activity of chrysotile and found that hemoglobin attached to chrysotile, but not silica, can work as a catalyst to induce oxidative DNA damage. This process generates 8-hydroxy-2¢-deoxyguanosine and thus corroborates the involvement of iron in the carcinogenicity of chrysotile. This evidence demonstrates that all three types of asbestos adsorb DNA and specific proteins, providing a niche for oxidative modification via catalytic iron. Therefore, considering the affinity of asbestos for histones ⁄ DNA and the internalization of asbestos into mesothelial cells, our results suggest a novel hypothetical mechanism causing genetic alterations during asbestos-induced carcinogenesis. (Cancer Sci 2011; 102: 2118-2125 A sbestos is a natural fibrous mineral that was heavily used in industry during the past century because of its durability, heat resistance and low cost. However, it has become clear that respiratory exposure to asbestos fibers, especially crocidolite and amosite, which have high biopersistence and contain abundant iron, is associated with high risks of developing malignant mesothelioma and lung cancer.(1-3) Many countries anticipate increased numbers of mesothelioma patients in the coming decades because there is an extremely long incubation period (30-40 years) for this fatal disease following asbestos exposure. (4) The molecular mechanism of asbestos-induced carcinogenesis remains elusive, (5) but both mesothelial cell injury and persistent macrophage activation are thought to be essential, if not sufficient, for mesotheliomagenesis.(6) These two events interact in vivo, leading to genetic mutations, chromosomal aberrations and aneuploidy in mesothelial cells. At least four major hypotheses related to the underlying mechanisms have been proposed. (6,7) First, the free radical theory postulates that DNA is injured by reactive oxygen species generated th...

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“…(59) Asbestos is known to cause hemolysis. (65) We confirmed this property using UICC asbestos and silica (Fig. 3a,b).…”

Section: Resultssupporting

confidence: 68%

Section: Discussionmentioning

confidence: 61%

Asbestos surface provides a niche for oxidative modification

et al. 2011

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“…Since chrysotile is coated with magnesium on which one of the positive charges is free, it is not surprising that the fiber will bind phospholipids and membranes; indeed, this had been postulated by McNab and Harington (15) and reported by Jaurand et al (10). The fusogenic properties of the fiber are not surprising, since it can be envisioned that conformational changes will occur related to the binding of the membrane to the magnesium.…”

Section: Discussionmentioning

confidence: 92%

Interaction of erythrocyte membranes with particulates.

Ottolenghi¹,

Joseph²,

Newman³

et al. 1983

Environ Health Perspect

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“…In vitro hemolysis has been observed with silica (Harley and Margolis, 1961;Charache et al, 1962;Nash et aL, 1966;Macnab and Harington, 1967), chrysotile (Secchi and Rezzonico, 1968;Schnitzer and Pundsack, 1970), sepiolite and palygorskite (Hayashi et al, 1969), kaolinite (Manyai et al, 1969(Manyai et al, , 1970, and montmorillonite and illite (Manyai et al, 1969).…”

Section: Introductionmentioning

confidence: 99%

Lysis of Erythrocytes by Silicate Minerals

Oscarson

1986

Clays and clay miner.

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Abstract--In vitro studies of the destruction (lysis) of bovine red blood cells (erythrocytes) by some silicate minerals showed the reaction to be complete in less than 1 hr and very destructive to the cell membrane. The activity as lysing agents was found to be in the order smectites > silica > palygorskite -~ sepiolite > chrysotile > kaolinite. Different compositions (Fe, A1, Mg, Li, vacancy) of the octahedral sheet of the smectite and fibrous clay minerals did not appreciably alter their hemolytic activity. The most active particle size range for kaolinite and montmoriUonite was 0.2-2 ~m. Structural folding of palygorskite reduced lysis suggesting that edge surfaces and silanol groups are important in this process. Aluminum oxides and hydroxides caused no lysis, and coatings of positively charged aluminum-hydroxy polymers on montmorillonite, silica, palygorskite, and kaolinite significantly reduced lysis.

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Haemolytic Activity of Asbestos and other Mineral Dusts

Cited by 92 publications

References 8 publications

Asbestos surface provides a niche for oxidative modification

Asbestos surface provides a niche for oxidative modification

Interaction of erythrocyte membranes with particulates.

Lysis of Erythrocytes by Silicate Minerals

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