Haemophilus parasuis serovar 5 Nagasaki strain adheres and invades PK-15 cells

Frandoloso, Rafael; Martínez-Martínez, Sonia; Gutiérrez-Martín, César B.; Ferri, Elías Fernando Rodríguez

doi:10.1016/j.vetmic.2011.07.022

Cited by 40 publications

(29 citation statements)

References 16 publications

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“…PK-15 cells have been shown especially useful for the study of infectious disease processes in swine [25], [30]. In order to investigate the expression patterns of porcine Coro1A under general conditions that imitate bacterial and viral infection, the immunostimulation assay was carried out in PK-15 cells using LPS, poly (I:C) and H.parasuis as the stimulators.…”

Section: Resultsmentioning

confidence: 99%

Porcine Coronin 1A Contributes to Nuclear Factor-Kappa B (NF-κB) Inactivation during Haemophilus parasuis Infection

et al. 2014

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Haemophilus parasuis (H.parasuis) is the etiological agent of porcine polyserositis and arthritis (Glässer's disease) characterized by fibrinous polyserositis, meningitis and polyarthritis, causing severe economic losses to the swine industry. Currently, the molecular basis of this infection is largely unkonwn. Coronin 1A (Coro1A) plays important roles in host against bacterial infection, yet little is known about porcine Coro1A. In this study, we investigated the molecular characterization of porcine Coro1A, revealing that porcine Coro1A was widely expressed in different tissues. Coro1A could be induced by lipopolysaccharide (LPS), polyinosinic acid-polycytidylic acid [poly (I:C)] and H.parasuis in porcine kidney-15 (PK-15) cells. Functional analyses revealed that porcine Coro1A suppressed the NF-κB activation during H.parasuis infection by inhibiting the degradation of IκBα and nuclear translocation of p65. Overexpression of porcine Coro1A inhibited the transcription of NF-κB-mediated downstream genes [Interleukin-6 (IL-6), Interleukin-8 (IL-8) and COX-2] through down-regulation of NF-κB. The results indicated that porcine Coro1A is an important immunity related gene that helps to inhibit NF-kB activation during H. parasuis infection.

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Section: Resultsmentioning

confidence: 99%

Porcine Coronin 1A Contributes to Nuclear Factor-Kappa B (NF-κB) Inactivation during Haemophilus parasuis Infection

et al. 2014

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“…Additionally, things will get worse in piglets co-infected with some other respiratory pathogens or immunosuppressive pathogens, for example, swine influenza virus could aggravate the lesions to the lung and thus facilitates microbial replication in the lung [28]. Despite of these external causes, H. parasuis also utilizes its internal factors for initial colonization, probably via adhesion to and invasion of host epithelial cells [29–31], disrupting mucosal immunity by degradation of IgA [32], resisting against phagocytosis by macrophage as well as bactericidal effect by the complement system [33–35]. Therefore, identification and characterization of the critical virulence factors that participating in these important phenotypes should provide better understanding of H. parasuis pathogenicity.…”

Section: Discussionmentioning

confidence: 99%

“…Both PK15 and PIEC cells were used to test the adhesion and invasion abilities of the wild-type, ΔlsgB mutant, and C- lsgB strains as previously described [29,31,54]. For the adhesion assay, bacteria were grown overnight in 5 mL TSB/V/S at 37°C with agitation.…”

Section: Methodsmentioning

confidence: 99%

Haemophilus parasuis α-2,3-sialyltransferase-mediated lipooligosaccharide sialylation contributes to bacterial pathogenicity

et al. 2018

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Bacterial lipooligosaccharide (LOS) is an important virulence-associated factor, and its sialylation largely confers its ability to mediate cell adhesion, invasion, inflammation, and immune evasion. Here, we investigated the function of the Haemophilus parasuis α-2,3-sialyltransferase gene, lsgB, which determines the terminal sialylation of LOS, by generating a lsgB deletion mutant as well as a complementation strain. Our data indicate a direct effect of lsgB on LOS sialylation and reveal important roles of lsgB in promoting the pathogenicity of H. parasuis, including adhesion to and invasion of porcine cells in vitro, bacterial load and survival in vivo, as well as a contribution to serum resistance. These observations highlight the function of lsgB in mediating LOS sialylation and more importantly its role in H. parasuis infection. These findings provide a more profound understanding of the pathogenic mechanism of this disease-causing bacterium.

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“…The pathogenesis factors include to invade the host, bacterial multiplication and damage to host tissue (Bouchet et al 2009). Comparisons in functional assays between virulent and non-virulent strains of H. parasuis allow the identification of several virulence mechanisms that help the bacterium colonise and initiate the infection by adhesion and invasion of epithelial cells (Frandoloso et al 2012). As other members of Pasteurellaceae family, H. parasuis can avoid phagocytosis, but the bacterial factors involved in the virulence are still unknown (Costa-hurtado et al 2013).…”

Section: Update About the Pathogenesismentioning

confidence: 99%

The challenges with Glässer’s disease in technified pig production

Pereira

Dalla-Costa

Ferroni

et al. 2017

Austral j. vet. sci.

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ABSTRACT. The swine upper respiratory tract is early colonised by Haemophilus parasuis, a bacteria which causes Glässer´s disease under favorable conditions. Glässer's disease is a septicemic infectious disease characterised by causing polyserositis. The prevention of Glässer disease still represents a big challenge for the production chain, since the mechanism of systemic infection in pigs and virulence factors that prevent phagocytosis are not yet well understood. Even in swine herds with high sanitary standard, it is the main cause of mortality that has led to productive and economic losses in the pig industry worldwide. Although the H. parasuis genome sequence has been completed already, diagnosis is still difficult due to the existence of non-virulent strains and the early colonisation of the upper respiratory tract of healthy swines. This review aims to provide up-to-date information about the etiology, epidemiology, pathogenesis, clinical signs, gross and microscopic lesions, diagnosis, treatment and control of Glässer's disease.Key words: epidemiology, swine, virulence factors, vaccines. RESUMEN.El tracto respiratorio superior del cerdo es colonizado inicialmente por Haemophilus parasuis, bacteria que en condiciones favorables causa la enfermedad de Glässer. La enfermedad de Glässer es una afección infecciosa que se caracteriza por el desarrollo de poliserositis septicémica. La prevención de esta enfermedad sigue siendo un problema en la producción porcina tecnificada, ya que los mecanismos de virulencia de este microorganismo y los factores sistémicos que impiden la fagocitosis no son bien conocidos. Inclusive en rebaños con un alto estándar de salud, H. parasuis es la principal causa de mortalidad, generando así pérdidas productivas y económicas en la industria porcina a nivel mundial. A pesar de que la secuencia del genoma de H. parasuis se ha completado recientemente, el diagnóstico aún se complica por la existencia de cepas no virulentas y la temprana colonización del tracto respiratorio superior de cerdos sanos. El objetivo de esta revisión es entregar información actualizada respecto de la etiología, epidemiología, patogénesis, signos clínicos, lesiones macroscópicas y microscópicas, diagnóstico, tratamiento y control de la enfermedad de Glässer.

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Haemophilus parasuis serovar 5 Nagasaki strain adheres and invades PK-15 cells

Cited by 40 publications

References 16 publications

Porcine Coronin 1A Contributes to Nuclear Factor-Kappa B (NF-κB) Inactivation during Haemophilus parasuis Infection

Porcine Coronin 1A Contributes to Nuclear Factor-Kappa B (NF-κB) Inactivation during Haemophilus parasuis Infection

Haemophilus parasuis α-2,3-sialyltransferase-mediated lipooligosaccharide sialylation contributes to bacterial pathogenicity

The challenges with Glässer’s disease in technified pig production

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Haemophilus parasuis serovar 5 Nagasaki strain adheres and invades PK-15 cells

Cited by 40 publications

References 16 publications

Porcine Coronin 1A Contributes to Nuclear Factor-Kappa B (NF-κB) Inactivation during Haemophilus parasuis Infection

Porcine Coronin 1A Contributes to Nuclear Factor-Kappa B (NF-κB) Inactivation during Haemophilus parasuis Infection

Haemophilus parasuis α-2,3-sialyltransferase-mediated lipooligosaccharide sialylation contributes to bacterial pathogenicity

The challenges with Glässer&#8217;s disease in technified pig production

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The challenges with Glässer’s disease in technified pig production