Hair dermatophytosis diagnosed by reflectance confocal microscopy: six cases

Cinotti, Élisa; Perrot, Jean; Labeille, B.; Rabérin, H.; Flori, Pierre; Cambazard, F.

doi:10.1111/jdv.12557

Cited by 28 publications

(36 citation statements)

References 8 publications

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“…Recently, the provision of a hand held compact camera (VivaScope ® 3000) permitting flexible movements in all directions has allowed a fast and easy diagnosis. Moreover, it enables the examination of all body areas due to its smaller tip, thus increasing the detection capability of infectious agents …”

Section: In Vivo Reflectance Confocal Microscopy Devicesmentioning

confidence: 99%

“…Therefore, RCM can allow to confirm the diagnosis of dermatophytosis during the dermatological consultation and to initiate an appropriate treatment without waiting for the conventional mycological examination . Dermatophytes are easily identified under RCM in the skin, the nail plate and hairs due to their particular appearance as thin, high‐reflective and longitudinal structures with a serpentine shape (Fig. ).…”

Section: Confocal Microscopy and Superficial Mycosismentioning

confidence: 99%

“…Our group recently demonstrated the possibility of RCM use not only to identify filaments but also conidia, which corresponds to roundish hyper‐reflective bright structures . The RCM aspect of yeasts in onychomycosis has been reported only by Arrese et al., while few cases of nail infestation by moulds have been included in the series of Pharaon et al., but no specific features are described.…”

Section: Confocal Microscopy and Superficial Mycosismentioning

confidence: 99%

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Reflectance confocal microscopy for cutaneous infections and infestations

Cinotti

Perrot

Labeille

et al. 2015

Acad Dermatol Venereol

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Reflectance confocal microscopy (RCM) is a high-resolution emerging imaging technique that allows non-invasive diagnosis of several cutaneous disorders. A systematic review of the literature on the use of RCM for the study of infections and infestations has been performed to evaluate the current use of this technique and its possible future applications in this field. RCM is particularly suitable for the identification of Sarcoptes scabies, Demodex folliculorum, Ixodes, Dermatophytes and Candida species in the clinical practice and for the follow-up after treatment. The cytopathic effect of herpes simplex virus, varicella zoster virus and molluscipoxvirus is also detectable by this imaging technique even in a pre-vesicular stage. In addition, thanks to its non-invasiveness, RCM allows pathophysiological studies.

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Section: In Vivo Reflectance Confocal Microscopy Devicesmentioning

confidence: 99%

Section: Confocal Microscopy and Superficial Mycosismentioning

confidence: 99%

Section: Confocal Microscopy and Superficial Mycosismentioning

confidence: 99%

See 1 more Smart Citation

Reflectance confocal microscopy for cutaneous infections and infestations

Cinotti

Perrot

Labeille

et al. 2015

Acad Dermatol Venereol

Self Cite

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show abstract

“…The use of RCM in tinea capitis was already described in a study where RCM was performed in six cases of hair dermatophytosis . The study outlined that RCM can confirm the diagnosis in a few minutes, identifying hair dermatophytes and that conidia are “visible” under RCM due to their high reflectance.…”

Section: Case Reportmentioning

confidence: 99%

Reflectance confocal microscopy of tinea capitis: comparing images with results of dermoscopy and mycological exams

Veasey¹,

Meneses

Silva

2018

Int J Dermatology

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“…In addition, it has been used in the examination of infectious and inflammatory skin diseases . A few reports also showed the applicability of ex vivo CLSM for internal organ disorders and skin appendages, such as hair and nails . A recent study published by our group combined fluorescent‐tagged antibodies in the ex vivo CLSM examination of skin tumors and showed a possible application of ex vivo CLSM as an alternative diagnostic method for immunohistochemical/immunofluorescence examination of the skin.…”

Section: Introductionmentioning

confidence: 99%

Ex vivo confocal laser scanning microscopy: An innovative method for direct immunofluorescence of cutaneous vasculitis

Bağcı

Aoki

Krammer

et al. 2019

Journal of Biophotonics

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Ex vivo confocal laser scanning microscopy (ex vivo CLSM) offers an innovative diagnostic approach through vertical scanning of skin samples with a resolution close to conventional histology. In addition, it enables fluorescence detection in tissues. We aimed to assess the applicability of ex vivo CLSM in the detection of vascular immune complexes in cutaneous vasculitis and to compare its diagnostic accuracy with direct immunofluorescence (DIF) microscopy. Eighty‐two sections of 49 vasculitis patients with relevant DIF microscopy findings were examined using ex vivo CLSM following staining with fluorescent‐labeled IgG, IgM, IgA, C3 and fibrinogen antibodies. DIF microscopy showed immunoreactivity of vessels with IgG, IgM, IgA, C3 and Fibrinogen in 2.0%, 49.9%, 12.2%, 59.2% and 44.9% of the patients, respectively. Ex vivo CLSM detected positive vessels with the same antibodies in 2.0%, 38.8%, 8.2%, 42.9% and 36.7% of the patients, respectively. The detection rate of positive superficial dermal vessels was significantly higher in DIF microscopy as compared to ex vivo CLSM (P < .05). Whereas, ex vivo CLSM identified positive deep dermal vessels more frequently compared to DIF microscopy. In conclusion, ex vivo CLSM could identify specific binding of the antibodies in vessels and showed a comparable performance to conventional DIF microscopy in diagnosing vasculitis.

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Hair dermatophytosis diagnosed by reflectance confocal microscopy: six cases

Cited by 28 publications

References 8 publications

Reflectance confocal microscopy for cutaneous infections and infestations

Reflectance confocal microscopy for cutaneous infections and infestations

Reflectance confocal microscopy of tinea capitis: comparing images with results of dermoscopy and mycological exams

Ex vivo confocal laser scanning microscopy: An innovative method for direct immunofluorescence of cutaneous vasculitis

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