Hairy roots of Datura candida × D. aurea: effect of culture medium composition on growth and alkaloid biosynthesis

Nussbaumer, P; Kapétanidis, I.; Christen, Philippe

doi:10.1007/s002990050415

Cited by 41 publications

(26 citation statements)

References 10 publications

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“…Experimental designs afford maximum information about supercritical fluid extraction in less experiments than a univariate development would have required. Experimental standard deviation in % (n = 6 for hyoscyamine and scopolamine and n = 9 for cocaine) c) Values reported in the literature for liquid-solid extractions of cocaine [24,[26][27][28] and of hyoscyamine and scopolamine [22] Fig 6. Response surface plots for scopolamine with 20 % MeOH.…”

Section: Resultsmentioning

confidence: 99%

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Application of central composite designs in the supercritical fluid extraction of tropane alkaloids in plant extracts

et al. 1999

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Abstract. Central composite designs were used in the study of supercritical fluid extraction (SFE) of three tropane alkaloids : hyoscyamine and scopolamine from Datura candida x D. aurea hairy roots and cocaine from coca leaves (Erythroxylum coca). Several SFE parameters, such as pressure, temperature, the nature and percentage of the polar modifier were examined. The tropane alkaloid extraction yield was established as response. A comparison between the predicted and the experimental extraction yields was established in order to assess the prediction quality of the model. Response surfaces were drawn from the mathematical models and enabled to determine the optimal conditions and to visualise the method robustness. The behaviour of the compounds investigated was discussed in relation with the selected SFE parameters.

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Section: Resultsmentioning

confidence: 99%

“…The culture of Datura candida x Datura aurea hairy roots was established after infection with Agrobacterium rhizogenes A 4 as already reported [22]. Leaves of E. coca var.…”

Section: Reagents and Materialsmentioning

confidence: 99%

Application of central composite designs in the supercritical fluid extraction of tropane alkaloids in plant extracts

et al. 1999

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“…The phenotypic characteristics of transformed roots include: rapid growth, hormone autotrophy, reduced apical dominance, high branching and root plagiotropism (Tepfer and Tempe 1981) and one of the unique characteristics of hairy roots is their enhanced, stable production of secondary metabolites (Bourgaud et al 2001). The sucrose level, exogenous growth hormone, the nature of the nitrogen source and their relative amounts, light, temperature and the presence of chemicals can all affect growth, total biomass yield, and secondary metabolite production (Rhodes et al 1994;Nussbbaumer et al 1998).…”

Section: Introductionmentioning

confidence: 99%

The influence of Agrobacterium rhizogenes on induction of hairy roots and ß-carboline alkaloids production in Tribulus terrestris L.

Sharifi

Sattari

Zebarjadi

et al. 2013

Physiol Mol Biol Plants

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We have developed an efficient transformation system for Tribulus terrestris L., an important medicinal plant, using Agrobacterium rhizogenes strains AR15834 and GMI9534 to generate hairy roots. Hairy roots were formed directly from the cut edges of leaf explants 10-14 days after inoculation with the Agrobacterium with highest frequency transformation being 49 %, which was achieved using Agrobacterium rhizogenes AR15834 on hormone-free MS medium after 28 days inoculation. PCR analysis showed that rolB genes of Ri plasmid of A. rhizogenes were integrated and expressed into the genome of transformed hairy roots. Isolated transgenic hairy roots grew rapidly on MS medium supplemented with indole-3-butyric acid. They showed characteristics of transformed roots such as fast growth and high lateral branching in comparison with untransformed roots. Isolated control and transgenic hairy roots grown in liquid medium containing IBA were analyzed to detect ß-carboline alkaloids by High Performance Thin Layer Chromatograghy (HPTLC).Harmine content was estimated to be 1.7 μg g −1 of the dried weight of transgenic hairy root cultures at the end of 50 days of culturing. The transformed roots induced by AR15834 strain, spontaneously, dedifferentiated as callus on MS medium without hormone. Optimum callus induction and shoot regeneration of transformed roots in vitro was achieved on MS medium containing 0.4 mg L −1 naphthaleneacetic acid and 2 mg L −1 6-benzylaminopurine (BAP) after 50 days. The main objective of this investigation was to establish hairy roots in this plant by using A. rhizogenes to synthesize secondary products at levels comparable to the wild-type roots.

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“…The ammonium/nitrate ratio controls the pH of growth media, stimulates morphogenesis and embryogenesis, and thus it is important in inducing callus formation in many woody plant cultures. However, all the effects of the culture medium differ from one species to another and from one compound to another [42][43][44]. Therefore, it is necessary to establish a reproducible externally applied NO 3 -/NH 4 + ratio for the stable production of large quantities of special metabolites.…”

Section: Introductionmentioning

confidence: 99%

Increasing the Anti-Addictive Piperidine Alkaloid Production of In Vitro Micropropagated Indian Tobacco by Nitrate Treatments

Vojnich¹,

Bányai²,

Máthé³

et al. 2017

J Plant Biochem Physiol

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Hairy roots of Datura candida × D. aurea: effect of culture medium composition on growth and alkaloid biosynthesis

Cited by 41 publications

References 10 publications

Application of central composite designs in the supercritical fluid extraction of tropane alkaloids in plant extracts

Application of central composite designs in the supercritical fluid extraction of tropane alkaloids in plant extracts

The influence of Agrobacterium rhizogenes on induction of hairy roots and ß-carboline alkaloids production in Tribulus terrestris L.

Increasing the Anti-Addictive Piperidine Alkaloid Production of In Vitro Micropropagated Indian Tobacco by Nitrate Treatments

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