Application of central composite designs in the supercritical fluid extraction of tropane alkaloids in plant extracts

Abstract

Abstract. Central composite designs were used in the study of supercritical fluid extraction (SFE) of three tropane alkaloids : hyoscyamine and scopolamine from Datura candida x D. aurea hairy roots and cocaine from coca leaves (Erythroxylum coca). Several SFE parameters, such as pressure, temperature, the nature and percentage of the polar modifier were examined. The tropane alkaloid extraction yield was established as response. A comparison between the predicted and the experimental extraction yields was established in order to assess the prediction quality of the model. Response surfaces were drawn from the mathematical models and enabled to determine the optimal conditions and to visualise the method robustness. The behaviour of the compounds investigated was discussed in relation with the selected SFE parameters.

Response Surface Optimized Ultrasonic-Assisted Extraction of Flavonoids from Sparganii Rhizoma and Evaluation of Their in Vitro Antioxidant Activities

“…In order to optimise the extraction conditions, including concentration of solvent, extraction time and ultrasonic power, response surface methodology (RSM) has been widely used. By establishing a mathematical model, RSM evaluates multiple parameters and their interactions using quantitative data, effectively optimising complex extraction procedures in a statistical way, thus reducing the number of experimental trials required [ 16 ]. Although RSM has been applied to optimise ultrasonic-assisted extraction of flavonoids in many studies [ 13 , 17 ], to the best of our knowledge, there are no reports yet about the application of RSM on ultrasonic-assisted extraction optimisation for the extraction of flavonoids from SR.…”

Optimisation of Ultrasound‐assisted Extraction of Puerarin and Total Isoflavones from Puerariae Lobatae Radix (Pueraria lobata (Wild.) Ohwi) with Response Surface Methodology

“…In order to optimise the extraction conditions, including temperature, extraction time, solvent-to-material ratio and concentration of solvents, response surface methodology (RSM) has been widely used Zhao et al, 2011). By establishing a mathematical model, RSM evaluates multiple parameters and their interactions using quantitative data, effectively optimising complex extraction procedures in a statistical way, thus reducing the number of experimental trials required (Brachet et al, 1999). Although RSM has been applied to optimise MAE and PSE extraction of flavonoids (Xiao et al, 2008;Hossain et al, 2011), to the best of our knowledge, there are no reports yet about the application of RSM on UAE optimisation for the extraction of flavonoids from PLR.…”

Application of response surface methodology to optimise ultrasonic‐assisted extraction of four chromones in Radix Saposhnikoviae

“…Response surface methodology (RSM) is an effective statistical technique for optimising complex extraction procedures (Nasri Nasrabadi and Razavi, 2010; Ndrade‐Eiroa et al ., 2010). This experimental methodology can use quantitative data to evaluate multiple parameters and their interactions by establishing a mathematical model (Brachet et al ., 1999). Therefore, it is less laborious and time‐consuming than other approaches required to optimise a process.…”