Optimisation of Ultrasound‐assisted Extraction of Puerarin and Total Isoflavones from Puerariae Lobatae Radix (<i>Pueraria lobata</i> (Wild.) Ohwi) with Response Surface Methodology

Wu, Yilin; Wang, Xinsheng; Fan, Enguo

doi:10.1002/pca.2349

Cited by 31 publications

(17 citation statements)

References 28 publications

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“…Ethanol (60%) was chosen as the lowest solvent concentration in this study. This solvent was chosen because previous studies showed that antioxidant compounds from plant sources were best extracted by 70%–80% ethanol concentrations [25–27]. In this study, the kenaf seeds extracted by 80% ethanol had better 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity assay results than the 100% ethanol kenaf seed extract (Table 1).…”

Section: Resultsmentioning

confidence: 82%

Binary Solvent Extraction System and Extraction Time Effects on Phenolic Antioxidants from Kenaf Seeds (Hibiscus cannabinusL.) Extracted by a Pulsed Ultrasonic-Assisted Extraction

Wong

Lau

Tan

et al. 2014

The Scientific World Journal

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The aim of this study was to determine the best parameter for extracting phenolic-enriched kenaf (Hibiscus cannabinus L.) seeds by a pulsed ultrasonic-assisted extraction. The antioxidant activities of ultrasonic-assisted kenaf seed extracts (KSE) were determined by a 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity assay, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging assay, β-carotene bleaching inhibition assay, and ferric reducing antioxidant power (FRAP) assay. Total phenolic content (TPC) and total flavonoid content (TFC) evaluations were carried out to determine the phenolic and flavonoid contents in KSE. The KSE from the best extraction parameter was then subjected to high performance liquid chromatography (HPLC) to quantify the phenolic compounds. The optimised extraction condition employed 80% ethanol for 15 min, with the highest values determined for the DPPH, ABTS, and FRAP assay. KSE contained mainly tannic acid (2302.20 mg/100 g extract) and sinapic acid (1198.22 mg/100 g extract), which can be used as alternative antioxidants in the food industry.

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Section: Resultsmentioning

confidence: 82%

Binary Solvent Extraction System and Extraction Time Effects on Phenolic Antioxidants from Kenaf Seeds (Hibiscus cannabinusL.) Extracted by a Pulsed Ultrasonic-Assisted Extraction

Wong

Lau

Tan

et al. 2014

The Scientific World Journal

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“…[10] It is less laborious and time-consuming by reducing the number of experimental trials, [11] which makes it widely applied in the extraction process of natural plant and microbiology cultivation research. [12][13][14] Previous investigation has shown Box-Behnken was a useful experimental design to fit a second-order polynomial equation to optimize the extraction process, [15][16][17] and the processing error was almost negligible. Only by reducing system error, the optimal polynomial equation was close to the actual situation.…”

Section: Introductionmentioning

confidence: 99%

OPTIMIZATION OF EXTRACTION TECHNOLOGY OF GENTIOPICROSIDE FROMGENTIANA STRAMINEAMAXIM USING RESPONSE SURFACE METHODOLOGY ON ACCOUNT OF HPLC

Dang

Chen

Shao

et al. 2014

Journal of Liquid Chromatography & Related Technologies

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& Extraction time, ratio of methanol aqueous solution to material weight, and methanol concentration for three factor and three level response surface methodology (RSM) was used to study the optimum extraction technology of gentiopicroside from Gentiana Straminea Maxim. The analysis was performed on a high performance liquid chromatography (HPLC) system with gentiopicroside peak areas as response values. A ternary quadratic polynomial model with good correlation (R 2 ¼ 0.9786) was constructed and employed to guide the gentiopicroside extraction process. The optimum extraction conditions were an optimized extract time of 79.4 min, a ratio of methanol aqueous solution to material weight of 23.7 mL=g, methanol concentration of 92.6%, and three times extraction. Under the optimization extraction technology conditions, the predictive value of gentiopicroside peak area of the sample solution was 2120.6, and only 2.03% differ from the mean measured value 2164.6, and the optimized mean extraction yield of gentiopicroside was 78.3 mg Á g À1 . Through solvent distribution method crystallization of the extractive from response surface experiments, the white crystalline compound gentiopicroside was prepared and its purity was greater than 95%.

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“…For solvent to feed ratios below 300, only reduced yields were achieved. Several authors also reported a dependency of extraction yield on solvent to feed ratio . Similarly, they found that an increase of solvent to feed ratio generally leads to an increase of the yield of the target compound.…”

Section: Resultsmentioning

confidence: 88%

Solvent accessibility limitation by plant matrix compounds in extraction of rutin from Solanum lycopersicum

Lück

Bergs

Jupke

2020

Separation Science Plus

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Extraction procedures for plant‐based secondary metabolites are mainly influenced by choice of solvent, particle size and solvent to feed ratio. For developing an extraction protocol design‐of‐experiments or model‐based approaches have widely been used. Botanical aspects such as the influence of side compounds are less investigated. Either for analytical or preparative purposes, knowledge of the underlying extraction mechanisms is required to improve the process development. In this work, we demonstrate the mechanism of accessibility limitation of rutin by side compounds depending on the solvent to feed ratio. Extraction yields of target and side compounds are analyzed to derive correlations. The role of side compounds as limiting factors for target compound accessibility is demonstrated by performing two extraction steps using three different plant species. The maximum extraction yield of rutin is reached using a solvent to feed ratio of at least 300 for all three investigated plant species, while side compounds in liquid state generally affect the extractability of rutin. Phosphatidylcholines are identified as possible accessibility‐limiting compounds. In conclusion, the yields of target and side compounds strongly depend on the solvent to feed ratio, while side compounds cover the target compound in dry matter, reducing its accessibility.

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Optimisation of Ultrasound‐assisted Extraction of Puerarin and Total Isoflavones from Puerariae Lobatae Radix (Pueraria lobata (Wild.) Ohwi) with Response Surface Methodology

Abstract: The RSM allows for optimising the extraction parameters such that maximum extraction of puerarin and total isoflavones was achieved experimentally.

Cited by 31 publications

References 28 publications

Binary Solvent Extraction System and Extraction Time Effects on Phenolic Antioxidants from Kenaf Seeds (Hibiscus cannabinusL.) Extracted by a Pulsed Ultrasonic-Assisted Extraction

Binary Solvent Extraction System and Extraction Time Effects on Phenolic Antioxidants from Kenaf Seeds (Hibiscus cannabinusL.) Extracted by a Pulsed Ultrasonic-Assisted Extraction

OPTIMIZATION OF EXTRACTION TECHNOLOGY OF GENTIOPICROSIDE FROMGENTIANA STRAMINEAMAXIM USING RESPONSE SURFACE METHODOLOGY ON ACCOUNT OF HPLC

Solvent accessibility limitation by plant matrix compounds in extraction of rutin from Solanum lycopersicum

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